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The comprehensive study on the modulation of airway mucosal immune system by hochuekkito

Research Project

Project/Area Number 16390201
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General internal medicine (including Psychosomatic medicine)
Research InstitutionKitasato University

Principal Investigator

YAMADA Haruki  Kitasato University, Kitasato Institute for Life Sciences, Professor, 北里生命科学研究所, 教授 (60096691)

Co-Investigator(Kenkyū-buntansha) KIYOHARA Hiroaki  Kitasato University, Kitasato Institute for Life Sciences, Associate Professor, 北里生命科学研究所, 准教授 (70161601)
NAGAI Takayuki  Kitasato University, Kitasato Institute for Life Sciences, Assistant Professor, 北里生命科学研究所, 講師 (00172487)
MATSUMOTO Tsukasa  Kitasato University, Kitasato Institute for Life Sciences, Assistant Professor, 北里生命科学研究所, 講師 (00173906)
MAEDA Tadakazu  Kitasato University, School of Science, Professor, 理学部, 教授 (90265728)
Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥12,900,000 (Direct Cost: ¥12,900,000)
Fiscal Year 2006: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2005: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥6,900,000 (Direct Cost: ¥6,900,000)
KeywordsHochuekkito / Mucosal Immune system / Peyer's patch / Lymphocytes / Homing / Comprehensive study / Microarray / 腸管免疫 / パイエル板 / 漢方薬 / 鼻腔粘膜免疫系 / 腸管免疫系 / 分泌型IgA
Research Abstract

Oral administration of Hochuekkito (HET) to early aged BALB/c mice, which were nasally sensitized with influenza hemagglutinin vaccine, significantly enhanced influenza virus-specific IgA and IgG antibody titers ill nasal cavity and sera, respectively. However, Juzentaihoto failed to show the enhancing activity. HET increased not only influenza virus-specific IgA antibody titer but also total IgA antibody titer in nasal cavity. These results suggest that HET can stimulate the mucosal immune system of upper respiratory tract, and results in enhancement of antigen-specific antibody response in upper respiratory mucosal and systemic immune systems.
When HET was administered to OVA immunized mice, OVA-specific IgA titers in intestinal and nasal washes were significantly enhanced by oral administration of HET. Microarray analysis of Peyer's patch cells revealed enhanced expression of L-selectin gene by HET. The increase in L-selectin positive lymphocytes was confirmed by flow cytometry analy … More sis. These results suggest that orally administered HET may strengthen defensive systems against many kinds of pathogens and food antigens in intestine, and the increase in the population of L-selectin positive lymphocytes by HET may partly contribute to enhanced IgA immune response against intestinal antigen.
Effect of HET on cytokine secretion of intestinal epithelial cells was investigated. When murine normal colonic epithelial cell line was stimulated with HET, the contents of G-CSF in the conditioned medium were significantly increased in a dose and time dependent manners. The enhanced G-CSF secretion by HET was also observed in primary cultured colonic epithelial cells. The polysaccharide fraction (F-5) of HET enhanced the G-CSF secretion of the cells, and the activity of F-5 disappeared after periodate oxidation which destroy carbohydrate moiety. These results suggest that HET enhances secretion of G-CSF from colonic epithelial cells and resulting G-CSF modulate mucosal immune system, and polysaccharide is one of active ingredients of HET. Less

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (23 results)

All 2007 2006 2005 2004

All Journal Article (23 results)

  • [Journal Article] An immunomodulating pectic polymer Glinus oppositifolius2007

    • Author(s)
      K T Inngierdingen, et al.
    • Journal Title

      Phytochemistry 68

      Pages: 1046-1058

    • Related Report
      2006 Annual Research Report
  • [Journal Article] The presence of natural human antibodies against pharmacologically active pectic polysaccharides from herbal medicines2006

    • Author(s)
      H.Kiyohara et al.
    • Journal Title

      Phytomedicine 13(7)

      Pages: 494-500

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Total synthesis, elucidation of absolute stereochemistry, and adjuvant activity of trihydroxy fatty acids2006

    • Author(s)
      T.Shirahata et al.
    • Journal Title

      Tetrahedron 62(40)

      Pages: 9483-9496

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Stimulating effect of Japanese herbal (Kampo) medicine, Hochuekkito on upper respiratory mucosal immune system2006

    • Author(s)
      H.Kiyohara et al.
    • Journal Title

      eCAM 3(4)

      Pages: 459-467

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] The presence of natural human antibodies against pharmacologically active pectic polysaccharides from herbal medicines2006

    • Author(s)
      H.Kiyohara, T.Matsumoto, T.Nagai, S-J.Kim, H.Yamada
    • Journal Title

      Phytomedicine 13(7)

      Pages: 494-500

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Total synthesis, elucidation of absolute stereochemistry, and adjuvant activity of trihydroxy fatty acids2006

    • Author(s)
      T.Shirahata, T.Sunazuka, K.Yoshida, D.Yamamoto, Y.Harigaya, I.Kuwajima, T.Nagai, H.Kiyohara, H.Yamada, S.Omura
    • Journal Title

      Tetrahedron 62(40)

      Pages: 9483-9496

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Stimulating effect of Japanese herbal (Kampo) medicine, Hochuekkito on upper respiratory mucosal immune system2006

    • Author(s)
      H.Kiyohara, T.Nagai, K.Munakata, K.Nonaka, S.J.Kim, H.Yamada
    • Journal Title

      eCAM 3(4)

      Pages: 459-467

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] The presence of human natural antibodies against pharmacologically active pectic polysaccharides herbal medicines2006

    • Author(s)
      H Kiyohara, et al.
    • Journal Title

      Phytomedicine 13

      Pages: 494-500

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Structures of Structure-Activity Relationships of Three Mitogenic and Complement Fixing Pectic Arabinogalactans from the Malian Antiulcer Plants Cochlospermum tinctorium A. Rich and Vernonia kotschyana Sch. Bip. ex Walp.2006

    • Author(s)
      CS.Nergard, et al.
    • Journal Title

      Biomacromolecules 7

      Pages: 71-79

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Stimulating effect of Japanese herbal(kampo)medicine, hochuekkito on upper respiratory mucosal immune system.2006

    • Author(s)
      H Kiyohara, et al.
    • Journal Title

      Evid.Based Complement Alternat 3

      Pages: 459-467

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Studies on elucidation of action mechanism and active ingredients of Kampo medicines by basic research2005

    • Author(s)
      H.Yamada et al.
    • Journal Title

      J. Trad Med. 22(5)

      Pages: 263-277

    • NAID

      130004495459

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Studies on elucidation of action mechanism and active ingredients of Kampo medicines by basic research2005

    • Author(s)
      H.Yamada
    • Journal Title

      J.Trad.Med. 22

      Pages: 263-277

    • NAID

      130004495459

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Structural and immunological studies of a pectin and a pectic arabinogalactan from Vernonia kotschyana Sch. Bip. ex Walp. (Asteraceae).2005

    • Author(s)
      CS Nergard, et al.
    • Journal Title

      Carbohydr Res. 340

      Pages: 115-130

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Medicinal use of Cochlospermum tinctorium in Mali Anti-ulcer-, radical scavenging- and immunomodulating activities of polymers in the aqueous extract of the roots.2005

    • Author(s)
      CS Nergard, et al.
    • Journal Title

      J Ethnopharmacol. 96

      Pages: 255-269

    • Related Report
      2005 Annual Research Report
  • [Journal Article] A possible signal transduction pathway for cyclin D2 expression by a pectic polysaccharide from the roots of Bupleurum falcatum L. in murine B cell.2005

    • Author(s)
      T Matsumoto, et al.
    • Journal Title

      Int Immunopharmacol. 5

      Pages: 1373-1386

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Structure-immunomodulating activity relationships of a pectic arabinogalactan from Vernonia kotschyana Sch. Bip. ex Walp.2005

    • Author(s)
      CS Nergard, et al.
    • Journal Title

      Carbohydr Res. 340

      Pages: 1789-1801

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Combination effect of component herbs of a Japanese herbal (Kampo) medicine, Juzen-taiho-to on expression of intestinal immune system, modulating activity2004

    • Author(s)
      H.Kiyohara et al.
    • Journal Title

      eCAM 1 (1)

      Pages: 83-91

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Anti-allergic activity of a Kampo (Japanese herbal) medicine "Sho-seiryu-to (Xiao-Qing-Long-Tang)" on airway inflammation in a mouse model2004

    • Author(s)
      T.Nagai et al.
    • Journal Title

      Int. Immunopharmacol. 4(10-11)

      Pages: 1353-1365

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Combination effect of component herbs of a Japanese herbal (Kampo) medicine, Juzen-taiho-to on expression of intestinal immune system, modulating activity2004

    • Author(s)
      H.Kiyohara, T.Matsumoto, H.Yamada
    • Journal Title

      eCAM. 1(1)

      Pages: 83-91

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Anti-allergic activity of a Kampo (Japanese herbal) medicine "Sho-seiryu-to (Xiao-Qing-Long-Tang)" on airway inflammation in a mouse model2004

    • Author(s)
      T.Nagai, Y.Arai, M.Emori, S.Nunome, T.Yabe, T.Takeda, H.Yamada
    • Journal Title

      Int.Immunopharmacol. 4(10-11)

      Pages: 1353-1365

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Anti-allergic activity of a Kampo (Japanese herbal) medicine "Sho-seiryu-to (Xiao-Qing-Long-Tang)" on airway inflammation in a mouse model2004

    • Author(s)
      Takayuki Nagai, Yumiko Arai, Michiko Emori, Shin-yu Nunome, Takeshi Yabe, Tadahiro Takeda, Haruki Yamada
    • Journal Title

      International Immunopharmacology 4

      Pages: 1353-1365

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Combination effects of Herbs in a multi-herbal formula : expression of Juzen-taiho-to's immuno-modulatory activity on the intestinal immune system2004

    • Author(s)
      Hiroaki Kiyohara, Tsukasa Matsumoto, Haruki Yamada
    • Journal Title

      Evidence-Based Complementary Alternative Medicine 1

      Pages: 83-91

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Structure of oligosaccharide side chains of an intestinal immune system modulating arabinogalactan isolated from rhizomes of Atractylodes lancea DC.2004

    • Author(s)
      Ikue Taguchi, Hiroaki Kiyohara, Tsukasa Matsumoto, Haruki Yamada
    • Journal Title

      Carbohydrate Research 339

      Pages: 763-770

    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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