| Project/Area Number |
16390245
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Kidney internal medicine
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| Research Institution | The University of Tokushima |
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Principal Investigator |
TSUKAGUCHI Hiroyasu The University of Tokushima, Graduate School Institute of Health Biosciences, Research Associate, 大学院ヘルスバイオサイエンス研究部, 助手 (60335792)
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| Co-Investigator(Kenkyū-buntansha) |
DOI Toshio The University of Tokushima, Graduate School Institute of Health Biosciences, Professor, 大学院ヘルスバイオサイエンス研究部, 教授 (60183498)
KAGAMI Shoji The University of Tokushima, Graduate School Institute of Health Biosciences, Professor, 大学院ヘルスバイオサイエンス研究部, 教授 (00224337)
IIJIMA Kazumoto National Center for Child Health and Development, Director, 小児腎臓科, 医長 (00240854)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥13,700,000 (Direct Cost: ¥13,700,000)
Fiscal Year 2005: ¥6,600,000 (Direct Cost: ¥6,600,000)
Fiscal Year 2004: ¥7,100,000 (Direct Cost: ¥7,100,000)
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| Keywords | nephrotic syndrome / podocytes / mutation / linkage analysis |
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| Research Abstract |
Familial steroid-resistant nephrotic syndrome (SRN) is characterized by early onset of proteinuria with rapid progression of renal failure and renal histology of focal segmental glomerulosclerosis (FSGS). Defects in genes encoding components of glomerular silt diaphragm, e.g., NPHS1, 2, and NEPH1, have been implicated in the pathogenesis. Even though NPHS1 and 2 mutations have been significantly involved in Caucasian families, the relevance in Asian counterparts remains unclear. To explore the genetic basis of Asian patients, we tested linkage of the 3 known nephrotic syndrome loci (NPHS1, 2, and NEPH1) in 15 recessive SRN families originating from Japan and Korea. Linkage analysis excluded the contribution of 3 genes in 32 out of total 45 samples (3 loci in 15 families). The other 13 samples (6 families at NPHS1, 4 at NPHS2, and 3 at NEPH1) were inconclusive on linkage analysis but direct-sequencing revealed no alterations, thereby excluding the loci in all 15 families. Our data underscored genetic heterogeneity and ethnic differences in the pathogenesis of FSGS, and suggests the presence of as yet unidentified genes in Asian patients.
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