Development of a novel therapy for brain infarction using a cell-penetrating protein exhibiting enhanced anti-cell death activity

• Project/Area Number: 16390257
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurology
• Research Institution: Nippon Medical School
• Principal Investigator: OHTA Shigeo Nippon Medical School, Graduate School of Medicine, Professor (00125832)
• Co-Investigator(Kenkyū-buntansha): KATSURA Ken-ichiro Nippon Medical School, Dept. Internal Medicine, Senior Assistant Professor (50297892) ; ASOH Sadamitsu Nippon Medical School, Institute of Gerontology, Assistant Professor (70167914) ; KAMINURA Naoomi Nippon Medical School, Institute of Gerontology, Assistant Professor (60283800)
• Project Period (FY): 2004 – 2006
• Project Status: Completed (Fiscal Year 2006)
• Budget Amount: ¥14,400,000 (Direct Cost: ¥14,400,000); Fiscal Year 2006: ¥3,500,000 (Direct Cost: ¥3,500,000); Fiscal Year 2005: ¥3,500,000 (Direct Cost: ¥3,500,000); Fiscal Year 2004: ¥7,400,000 (Direct Cost: ¥7,400,000)
• Keywords: mitochondria / PTD-FNK / brain infarction / ischemia / Protein therapy / アポトーシス / 蛋白質導入治療 / Bcl-2ファミリー / 活性酸素 / 虚血・再灌流 / 細胞死 / Bck-xL / 蛋白質導入

Research Abstract

Many practical therapies have been explored for clinical applications to ischemic cerebral infarction; however, the most are still insufficient for treatments for acute stroke. We show here a potential combination therapy in a rat focal ischemic model to improve neurological symptoms as well as reduce infarct volumes at the maximum level. We applied a protein transduction technology using the artificial anti-death FNK protein fused with a protein-transduction-domain peptide (PTD-FNK). When PTD-FNK was administrated at1hr after initiating ischemia followed by the administration of an immunosuppressant FK506 with a 30-min time lag, infarct volumes of the total brain and cortex were markedly reduced to a level of 27% and 14%, respectively. This procedure not only reduced the infarct volume and edema, but also markedly improved neurological symptoms. The therapeutic effect continued at least for 1 week after ischemia. FK506 inhibited the transduction of PTD-FNK in vitro, which explains the requirement of a time lag for the administration of FK506. An additional in vitro experiment showed that PTD-FNK, when administered 30 min before FK506, gave the maximal protective effect through reducing an intracellular calcium concentration. We propose that this combination therapy would give a synergistic protective effect of both drugs, reducing adverse effect of FK506.

Research Products

• [Journal Article] PTD-mediated delivery of anti-cell death proteins/peptides and therapeutic enzymes. (2008)
  • Author(s): Asoh S., et al.
  • Journal Title: Adv. Drug Deliv. Rev. 60 Pages: 499-516
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Prevention of Chemotherapy-Induced Alopecia by anti-death FNK protein. (2008)
  • Author(s): Nakashima-Kamimura, N., et al.
  • Journal Title: Life Sci. 82 Pages: 218-225
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Combination therapy with transductive anti-death FNK protein and FK506 ameliorates brain damage with focal transient ischemia in rat. (2008)
  • Author(s): Katsura KI, et al.
  • Journal Title: J. Neurochem. (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Therapeutic benefits of intrathecal protein therapy in a mouse model of amyotrophic lateral sclerosis (2008)
  • Author(s): Ohta Y, et al.
  • Journal Title: J. Neurosci. Res. (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] PTD-mediated delivery of anti-cell death proteins/ peptides and therapeutic enzymes. (2008)
  • Author(s): Asoh S, et al.
  • Journal Title: Adv. Drug Deliv. Rev. 60 Pages: 499-516
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Prevention of Chemotherapy-Induced Alopecia by anti-death FNK protein. (2008)
  • Author(s): Nakashima-Kamimura N, et al.
  • Journal Title: Life Sci. 82 Pages: 218-225
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radicals (2007)
  • Author(s): Ohsawa I., et al.
  • Journal Title: Nat Med. 13 Pages: 688-694
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Anti-apoptotic PTD-FNK protein suppresses lipopolysaccharide-induced acute lung iniury in rats Experimental and Molecular Pathology (2007)
  • Author(s): Chen, H., et al.
  • Journal Title: Exp Mol. Pathol. 82 Pages: 218-225
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] A protein derived from the fusion of TAT peptide and FNK, a Bcl-X_L derivative, prevents cochlear hair cell death from aminoglycoside ototoxicity in vivo. (2007)
  • Author(s): Arakawa, M., et al.
  • Journal Title: Life Sci. 80 Pages: 2076-2084
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Prevention of Chemotherapy-Induced Alopecia by anti-death FNK protein. (2007)
  • Author(s): Kashio, A., et al.
  • Journal Title: Life Sci. 85 Pages: 1403-1412
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Transduction of the anti-apoptotic FTD-FNK protein improves the efficiency of transplantation of bone marrow mononuclear cells. (2007)
  • Author(s): Tara, S., et al.
  • Journal Title: J. Mol. Cell. Cardiol. 42 Pages: 489-497
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Protection of hepatic cells from apoptosis induced by ischemia/reperfusion injury by protein therapeutics. (2007)
  • Author(s): Nagai, S., et al.
  • Journal Title: Life Sci. 82 Pages: 218-225
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radicals. (2007)
  • Author(s): Ohsawa I., et al.
  • Journal Title: Nat Med. 13(6) Pages: 688-694
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Anti-apoptotic PTD-FNK protein suppresses lipopolysaccharid -induced acute lung injury in rats Experimental and Molecular Pathology. (2007)
  • Author(s): Chen, H., et al.
  • Journal Title: Exp Mol Pathol. 83 Pages: 377-384
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Transduction of anti-cell death protein FNK protects isolate' rat hearts from myocardial infarction induced by ischemia/ reperfusion. (2007)
  • Author(s): Arakawa M, et al.
  • Journal Title: Life Sci. 80 Pages: 2076-2084
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A protein derived from the fusion of TAT peptide and FNK, a Bel -x(L) derivative, prevents cochlear hair cell death from aminoglycoside ototoxicity in vivo. (2007)
  • Author(s): Kashio A, et al.
  • Journal Title: J Neurosci Res. 85 Pages: 1403-1412
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Transduction of the anti-apoptotic PTD-FNK protein improves th efficiency of transplantation of bone marrow mononuclear cells. (2007)
  • Author(s): Tara S, et al.
  • Journal Title: J Mol Cell Cardiol. 42 Pages: 489-497
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Protection of hepatic cells from apoptosis induced by ischemia/ reperfusion injury by protein therapeutics. (2007)
  • Author(s): Nagai S, et al.
  • Journal Title: Hepatol. Res. 37 Pages: 133-142
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Transduction of the anti-apoptotic PTD-FNK protein improves the efficiency of transplantation of bone marrow mononuclear cells. (2007)
  • Author(s): Tara, S., Miyamoto, M., Asoh, S, Ishii, N., Yasutake, M., Takagi, G., TAkano, T., Ohta,S.
  • Journal Title: J.Mol Cell Cardiol 42・3 Pages: 489-497
• [Journal Article] Protection of hepatic cells from apoptosis induced by ischemia/reperfusion injury by protein therapeutics. (2007)
  • Author(s): Nagai, S., Asoh, S., Kobayashi, Y., Shidara, Y., Mori, T., Suzuki, M., Moriyama, Y., Ohta,S.
  • Journal Title: Hepatol.Res. 37・2 Pages: 133-142
  • NAID: 10018567444
• [Journal Article] Association of alcohol dehydrogenase 2*1 allele with liver damage and insulin concentration in the Japanese. (2006)
  • Author(s): Suzuki Y., et al.
  • Journal Title: J. Hum. Genet. 51 Pages: 31-37
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Contribution of somatic mutations in the mitochondrial genome to the development of cancer and tolerance against anticancer drugs. (2006)
  • Author(s): Ohta S.
  • Journal Title: Oncogene 25 Pages: 4768-4776
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Dysfunction of mitochondria and oxidative stress in the pathogenesis of Alzheimer's disease : On defects in the cytochrome c oxidase complex and aldehyde detoxification. (2006)
  • Author(s): Ohta, S., et al.
  • Journal Title: J. Alzheimer's Dis. . 9 Pages: 155-166
  • Description: 「研究成果報告書概要(和文)」より
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• [Journal Article] Contribution of somatic mutations in the mitochondrial genome to the development of cancer and tolerance against anticancer drugs. (2006)
  • Author(s): Ohta, S.
  • Journal Title: Oncogene 25・34 Pages: 4768-4776
• [Journal Article] Dysfunction of mitochondria and oxidative stress in pathogenesis of Alzheimer's disease : On defects in the cytochrome c oxidase complex and aldehyde detoxification. (2006)
  • Author(s): Ohta, S., Ohsawa, I
  • Journal Title: J.Alzheimer's Disease 9・2 Pages: 155-166
• [Journal Article] Association of alcohol dehydrogenase 2^*1 allele with liver damage and insulin concentration in the Japanese. (2006)
  • Author(s): Suzuki, Y., Ando, F., Ohsawa, I., Shimokata, H., Ohta,S.
  • Journal Title: J.Hum.Genet. 51・1 Pages: 31-37
  • NAID: 10020558185
• [Journal Article] Asociation of alcohol dehydrogenase 2*1 allele with liver damage and insulin concentration in the Japanese. (2006)
  • Author(s): Suzuki Y
  • Journal Title: J Hum Genet. 51・1 Pages: 31-37
• [Journal Article] The anti-cell death FNK protein protects cells from death induced by freezing and thawing. (2005)
  • Author(s): Sudo, K., et al.
  • Journal Title: Biochem. Biophys. Res. Commun. 330 Pages: 850-856
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Zonal necrosis prevented by transduction of the artificial anti-death FNK protein (2005)
  • Author(s): Asoh S., et al.
  • Journal Title: Cell. Death Differ. 12 Pages: 384-394
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] The anti-cell death FNK protein protects cells from death induced by freezing and thawing. (2005)
  • Author(s): Sudo K., et al.
  • Journal Title: Biochem Biophys Res Commun. 13 Pages: 850-856
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Zonal necrosis prevented by transduction of the artificial antideath FNK protein (2005)
  • Author(s): Asoh S, et al.
  • Journal Title: Cell Death Differ. 12 Pages: 384-394
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] GPP34, a nuclear gene product, regulates total mitochondrial mass in response to mitochondrial dysfunction. (2005)
  • Author(s): Nakashima-Kamimura N
  • Journal Title: J Cell Sci. 118・22 Pages: 5357-5367
• [Journal Article] Prognostic significance of the immunohistochemical index of survivin in glioma : a comparative study with the MIB-1 index. (2005)
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  • Journal Title: J Neurooncol. 72・3 Pages: 231-238
• [Journal Article] Wobble modification deficiency in mutant tRNAs in patients with mitochondrial diseases. (2005)
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  • Journal Title: FEBS Lett. 579・13 Pages: 2948-2952
• [Journal Article] The anti-cell death FNK protein protects cells from death induced by freezing and thawing. (2005)
  • Author(s): Sudo K.
  • Journal Title: Biochem Biophys Res Commun. 330・3 Pages: 850-856
• [Journal Article] Zonal necrosis prevented by transduction of the artificial anti-death FNK protein. (2005)
  • Author(s): Asoh S
  • Journal Title: Cell Death Differ 12・4 Pages: 384-394
• [Journal Article] Zonal necrosis prevented by transduction of the artificial anti-death FNK protein. (2005)
  • Author(s): Asoh S, Mori T, Nagai S, Yamagata K, Nishimaki K, Miyato Y, Shidara Y, Ohta S.
  • Journal Title: Cell Death Differ. (in press)
• [Journal Article] The anti-cell death FNK protein protects cells from death induced by freezing and thawing. (2005)
  • Author(s): Sudo, K, Asoh, S, Ohsawa, I, Ozaki, D, Yamagata K, Ito H, Ohta S.
  • Journal Title: Biochem.Biophys.Res.Commun. (in press)
• [Journal Article] Mitochondrial tRNA(Leu(UUR)) mutation at position 3243 detected in patients with type 1 diabetes. (2005)
  • Author(s): Suzuki Y, Atsumi Y, Matsuoka K, Nishimaki K, Ohta S, Taniyama M, Muramatsu T.
  • Journal Title: Diabetes Res Clin Pract. 67・1 Pages: 92-94
• [Journal Article] Acute metabolic cataract as a first manifestation of deabetes mellitus in a 12-year-old girl. (2005)
  • Author(s): Suzuki Y, Atsumi Y, Matsuoka K, Nishimaki K, Ohta S, Taniyama M, Mutamatsu T.
  • Journal Title: Diabetologia 47・3 Pages: 592-593
• [Journal Article] Direct contribution of mutations in the mitochondrial genome to the promotion of cancer. (2005)
  • Author(s): Ohta S, SHidara Y, Yamagata K, Oda H.
  • Journal Title: Ann.N.Y.Acad.Sci. (in press)
• [Journal Article] Prognostic significance of the immunohistchemical index of surviving in glioma : A Comparative study with the MIB-1 index. (2005)
  • Author(s): Uematsu M, Ohsawa I, Aokage T, Nishimaki K, Matsumoto K, Takahashi H, Asoh S, Teramoto A, Ohta S.
  • Journal Title: J.Neuro-Oncology (in press)
• [Journal Article] Positive Contribution of Pathogenic Mutations in the Mitochondrial Genome to the Promotion of Cancer by Prevention from Apoptosis. (2005)
  • Author(s): Shidara Y, Yamagata K, Kanamori T, Nakano K, Kwong JQ., Manfredi, G, Oda H, Ohta S.
  • Journal Title: Cancer Res. (in press)
• [Journal Article] Transduction of anti-apoptotic proteins into chondrocytes in cartilage slice culture. (2004)
  • Author(s): Ozaki, D., et al.
  • Journal Title: Biochem. Biophys. Res. Commun. 313 Pages: 522-527
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Transduction of anti-apoptotic proteins into chondrocytes in cartilage slice culture. (2004)
  • Author(s): Ozaki D, et al.
  • Journal Title: Biochem. Biophys. Res .Commun. 313 Pages: 522-527
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Codon-specific translational defect caused by a wobble modification deficiency in mutant tRNA from a human mitochondrial disease. (2004)
  • Author(s): Kirino Y, Yasukawa T, Ohta S, Akira S, Ishihara K, Watanabe K, Suzuki T.
  • Journal Title: Proc.Natl.Acad.Sci.USA. 101 Pages: 15070-15075
• [Journal Article] Mitochondrial ALDH2 deficiency as an oxidative stress. (2004)
  • Author(s): Ohta S, Ohsawa I, Kamino K, Ando F, SHimokata H.
  • Journal Title: Ann.N.Y.Acad.Sci. 1011 Pages: 36-44
• [Journal Article] ALDH2/ADH2 Polymorphism Associated with Vasculopathy and Neuropathy in Type 2 Diabetes. (2004)
  • Author(s): Suzuki Y, Taniyama M, Muramatsu T, Higuchi S, Ohta S, Atsumi Y, Matsuoka K.
  • Journal Title: Alcohol Clin Exp Res. 28・8
• [Journal Article] Mitochondrial Genome Variation in Eastern Asia and the Peopling of Japan. (2004)
  • Author(s): Tanaka M, Cabrera VM, Gonzalez AM, Larruga JM, Takeyasu T, Fuku N, Fuo LJ, Hirose R, Fujita Y, Kurata M, Shinoda K, Umetsu K, Yamada Y, Oshida Y, Sato Y, Hattori N, Mizuno Y, Arai Y, Hirose N, Ohta S, Ogawa O, Tanaka Y, Kawamori R, Shamoto-Nagai M, Maruyama W, Shimokata H, Suzuki R, Shimodaira H.
  • Journal Title: Genome Res. 14・10A Pages: 1832-1850
• [Journal Article] Accumulation of somatic mutation in mitochondrial DNA and atherosclerosis in diabetic patients. (2004)
  • Author(s): Nomiyama T, Tanaka Y, Piao L, Hattori N, Uchino H, Watada H, Kawamori R, Ohta S.
  • Journal Title: Ann N Y Acad Sci. 1011 Pages: 193-204
• [Journal Article] Endurance exercise training inhibits activity of plasma GOT and liver caspase-3 of mice [correction of rats] exposed to stress by induction of heat shock protein 70. (2004)
  • Author(s): Mikami T, Sumida S, Ishibashi Y, Ohta S.
  • Journal Title: J Appl Physiol. 96・5 Pages: 1776-1781
• [Journal Article] Lipoma and opthalmoplegia in mitochondrial diabetes associated with small heteroplasmy level of 3243 tRNA(Leu(UUR)) mutation. (2004)
  • Author(s): Suzuki Y, Nishimaki K, Taniyama, M, T.Muramatsu T, Y.Atsumi Y, K.Matuoka K, Ohta S.
  • Journal Title: Diabetes Res Clin Pract. 63・3 Pages: 225-229
• [Journal Article] Combination therapy with transductive anti-death FNK protein and FK506 ameliorates brain damage with focal transient ischemia in rat.
  • Author(s): Katsura, KL., et al.
  • Journal Title: J. Neurochem. (In press)
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Therapeutic benefits of intrathecal protein therapy in a mouse model of amyotrophic lateral sclerosis
  • Author(s): Ohta, Y., et al.
  • Journal Title: J. Neurosci. Res. (In press)
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Hydrogen acts as a therapeutic anitioxidant by the selective reduction of cytotoxic oxygen radicals.
  • Author(s): Ohsawa, I., Ishikawa, M., Takahashi, K., Watanabe, M., Nishismaki, K., Yamagata, K., Katsura, K., Katayama, Y., Asoh, S., Ohta,S.
  • Journal Title: Nat.Med. (in press)
• [Journal Article] Transduction of anti-cell death protein FNK protects isolated rat hearts from myocardial infarction induced by ischemia/reperfusion
  • Author(s): Arakawa, M, Yasutake, M., Asoh, S., Miyamoto, M., Takano, T., Ohta,S
  • Journal Title: Life Sci. (in press)
• [Journal Article] A Protein Derived From the Fusion of TAT Peptide and FNK,a Bcl-Xl Derivative,prevents Cochlear Hair Cell Death From Aminoglyoside Ototoxicity In Vivo.
  • Author(s): Kashio, A., Sakamoto, T., Suzukawa, K., Asoh, S., Ohta, S., Yamasoba,T.
  • Journal Title: J.Neurosci,Res. (in press)
• [Presentation] 細胞死抑制活性強化タンパク質を用いたタンパク質導入治療法の開発 (2007)
  • Author(s): 太田 成男
  • Organizer: 日本薬学会・第8回創薬ビジョンシンポジウム
  • Place of Presentation: 日本・京都テルサ
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Development of Protein Transduction therapy using a protein with Anti-celldeath activity enhanced. (2007)
  • Author(s): OhtaN S.
  • Organizer: The 8th drug development vision symposium (Pharma ceutical Society of Japan)
  • Place of Presentation: Kyoto.
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] ミトコンドリアから発せられる活性酸素の消去 (2006)
  • Author(s): 太田 成男
  • Organizer: 第11回酸素ダイナミクス研究会
  • Place of Presentation: 日本・東京島津製作所イベントホール
  • Year and Date: 2006-09-30
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Alleviation of chemotherapeutic side effects by selective TAT-mediating delivery of an anti-cell death protein. International mini Symposium-Membrane-permeable peptides (2006)
  • Author(s): Ohta, Shigeo
  • Organizer: Chemistry, biology and therapeutic applications
  • Place of Presentation: 日本・京都大学薬学部
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Anti-apoptotic protein FNK debilitates side effects of anti-cancer drugs without inhibiting their anti-cancer activity (2006)
  • Author(s): Asoh, Sadamitsu
  • Organizer: Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress
  • Place of Presentation: 日本・京都国際会議場
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] ミトコンドリアはどこまで病気と健康に関与するか? (2006)
  • Author(s): 太田 成男
  • Organizer: 第6回日本ミトコンドリア学会年会
  • Place of Presentation: 日本・横浜情報化センター
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 細胞死抑制活性強化蛋白質FNKは抗癌剤の抗癌活性を抑制することなくその副作用を軽減・抑制する (2006)
  • Author(s): 麻生 定光
  • Organizer: 第15回アポトーシス研究会
  • Place of Presentation: 日本・ぱるるプラザ京都
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Alleviation of chemotherapeutic side effects by selective TATmediating delivery of an anti-cell death protein. International mini Symposium-Membrane-permeable peptides (2006)
  • Author(s): Ohta, S.
  • Organizer: Chemistry, biology and therapeutic applications.
  • Place of Presentation: Kyoto
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Anti-apoptotic protein FNK debilitates side effects of anti-cancer drugs without inhibiting their anti-cancer activity. (2006)
  • Author(s): Asoh, S.
  • Organizer: 20th IUBMB International Congress of Biochem. Mol. Biol. and 11th FAOBMB Congress.
  • Place of Presentation: Kyoto.
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] How are mitochondria with disease and health? (2006)
  • Author(s): Ohta, S.
  • Organizer: The 6th The Japanese Society of Mitochondrial Research and Medicine
  • Place of Presentation: Yokohama.
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Scavenging reactive oxygen species generated at mitochondria (2006)
  • Author(s): Ohta, S.
  • Organizer: The 11th Oxygen Dynamics Society
  • Place of Presentation: Tokyo.
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] The anti-cell death protein FNK targeted to mitochondria reduces side effects of anti-cancer drugs without affecting their anti-cancer activity. (2006)
  • Author(s): Asoh S.
  • Organizer: The 15th Japanese Society for Apoptosis
  • Place of Presentation: Kyoto.
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] The anti-cell death protein FNK targeted to mitochondria reduces side effects of anti-cancer drugs without affecting their anti-cancer activity. (2005)
  • Author(s): Asoh, Sadamitsu
  • Organizer: International Conference on Mitochondria and Life2005
  • Place of Presentation: 日本・Toshi Center Hotel
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] The anti-cell death protein FNK targeted to mitochondria reduces side effects of anti-cancer drugs without affecting their anti-cancer activity. (2005)
  • Author(s): Asoh, S.
  • Organizer: nternational Conference on Mitochondria and Life 2005
  • Place of Presentation: tokyo
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Studies on multiple functional mitochondria from Basic aspects towards Medical applications. (2005)
  • Author(s): Ohta, S.
  • Organizer: FinMIT/J-Mit Joint Meeting
  • Place of Presentation: Finland.
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] ミトコンドリアのちから (2008)
  • Author(s): 太田成男, 瀬名秀明
  • Total Pages: 423
  • Publisher: 新潮社
  • Description: 「研究成果報告書概要(和文)」より
• [Remarks] 「研究成果報告書概要(和文)」より
  • URL: http://www.ig.nms.ac.jp/
• [Patent(Industrial Property Rights)] 細胞死抑制活性強化蛋白質FNKを用いた音響外傷治療剤 (2005)
  • Inventor(s): 太田成男, 麻生定光
  • Industrial Property Rights Holder: 日本医科大学
  • Filing Date: 2005-02-09
  • Description: 「研究成果報告書概要(和文)」より
• [Patent(Industrial Property Rights)] 細胞死抑制活性強化蛋白質FNKを用いた骨随細胞、臍帯血細胞結保存と移植の効率の増強 (2005)
  • Inventor(s): 太田成男, 麻生定光, 太良修平
  • Industrial Property Rights Holder: 日本医科大学
  • Filing Date: 2005-03-14
  • Description: 「研究成果報告書概要(和文)」より
• [Patent(Industrial Property Rights)] 細胞死抑制活性強化タンパク質FNKを用いた脱毛の防止 (2005)
  • Inventor(s): 太田成男, 麻生定光, 上村尚美
  • Industrial Property Rights Holder: 日本医科大学
  • Filing Date: 2005-03-14
  • Description: 「研究成果報告書概要(和文)」より
• [Patent(Industrial Property Rights)] 細胞死抑制活性強化タンパク質FNKまたはそれをコードする核酸を含む抗癌治療の細胞毒性に基づく副作用の予防またに治療薬 (2005)
  • Inventor(s): 太田成男, 麻生定光, 渡邊健一
  • Industrial Property Rights Holder: 日本医科大学
  • Filing Date: 2005-08-23
  • Description: 「研究成果報告書概要(和文)」より
• [Patent(Industrial Property Rights)] 細胞死抑制活性強化タンパク質FNKまたはそれをコードする核酸を含む抗癌治療の細胞毒性に基づく副作用の予防または治療剤 (2005)
  • Inventor(s): 太田成男, 麻生定光, 渡邊健一
  • Industrial Property Rights Holder: 日本医科大学
  • Industrial Property Number: 2005-241685
  • Filing Date: 2005-08-23