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Visualization of insulin granules in pancreatic beta cells derived from genetically altered mouse models.

Research Project

Project/Area Number 16390261
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Metabolomics
Research InstitutionGunma University

Principal Investigator

IZUMI Testuro  Gunma University, Department of Molecular medicine, Institute for Molecular and Cellular Regulation, Professor, 生体調節研究所, 教授 (00212952)

Co-Investigator(Kenkyū-buntansha) GOMI Hiroshi  Gunma University, Department of Molecular medicine, Institute for Molecular and Cellular Regulation, Associated professor, 生体調節研究所, 助教授 (90293240)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥13,800,000 (Direct Cost: ¥13,800,000)
Fiscal Year 2005: ¥5,800,000 (Direct Cost: ¥5,800,000)
Fiscal Year 2004: ¥8,000,000 (Direct Cost: ¥8,000,000)
KeywordsInsulin / Secretory granules / Regulated exocytosis / Fluorescent protein / Mutated mouse
Research Abstract

We investigated the exocytosis of insulin granules in Rab27a-mutated ashen mice and granuphilin-knockout mice. We found that both Rab27a and its effector granuphilin are involved in the docking of insulin granules onto the plasma membrane. We then visualized insulin granules by expressing green fluorescent protein (GFP)-fused phogrin in a beta cell-derived cultured cell line. When granuphilin was overexpressed in the cell, the visualized granules were redistributed to the peripheral area close to the plasma membrane. We also tried to monitor the fusion of insulin granules by expressing insulin fused with two different fluorescent proteins, Ins-C-Timer and Ins-GFP. We found that the latter protein is more suitable to monitor the fusion and currently perform the imaging analyses in ashen or granuphilin-null beta cells.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report

Research Products

(15 results)

All 2005 2004 Other

All Journal Article

  • [Journal Article] Rab27a mediates the tight docking of insulin granules onto the plasma membrane during glucose stimulation.2005

    • Author(s)
      Kasai K., et al.
    • Journal Title

      J. Clin. Invest. 115

      Pages: 388-396

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Functional analysis of Rab27a effector granuphilin in insulin exocytosis.2005

    • Author(s)
      Izumi T., et al.
    • Journal Title

      Methods Enzymol. 403

      Pages: 216-229

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Granuphilin molecularly docks insulin granules to the fusion machinery.2005

    • Author(s)
      Gomi H., et al.
    • Journal Title

      J. Cell. Biol. 171

      Pages: 99-109

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Cytoplasmic transport signal is involved in phogrin targeting and localization to secretory granules.2005

    • Author(s)
      Torii S., et al.
    • Journal Title

      Traffic 6

      Pages: 1213-1224

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Rab27a mediates the tight docking of insulin granules onto the plasma membrane during glucose stimulation.2005

    • Author(s)
      Kasai K., et al.
    • Journal Title

      J.Clin.Invest. 115

      Pages: 388-396

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Granuphilin molecularly docks insulin granules to the fusion machinery.2005

    • Author(s)
      Gomi H., et al.
    • Journal Title

      J.Cell.Biol. 171

      Pages: 99-109

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Functional analysis of Rab27a effector granuphilin in insulin exocytosis.2005

    • Author(s)
      Izumi T., et al.
    • Journal Title

      Methods Enzymol. 390 Part G(in press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Rab27b localizes to zymogen granules and regulates pancreatic acinar exocytosis.2004

    • Author(s)
      Chen X., et al.
    • Journal Title

      Biochem. Biophys. Res. Commun. 323

      Pages: 1157-1162

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Secretogranin III binds to cholesterol in the secretory granule membrane as an adapter for chromogranin A.2004

    • Author(s)
      Hosaka M., et al.
    • Journal Title

      J.Biol.Chem. 279

      Pages: 3627-3634

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Functional localization of proprotein-convertase furin and its substrate TGFβ in EGF receptor-expressing gastric chief cells.2004

    • Author(s)
      Fujisawa T., et al.
    • Journal Title

      Growth Factors 22

      Pages: 51-59

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Rab27 effector granuphilin promotes the plasma membrane targeting of insulin granules via interaction with syntaxin 1a.2004

    • Author(s)
      Torii S., et al.
    • Journal Title

      J.Biol.Chem. 279

      Pages: 22532-22538

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Rab27b localizes to zymogen granules and regulates pancreatic acinar exocytosis.2004

    • Author(s)
      Chen X., et al.
    • Journal Title

      Biochem.Biophys.Res.Commun. 323

      Pages: 1157-1162

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Chromosome 2 locus Nidd5 has a potent effect on adiposity in the TSOD mouse.

    • Author(s)
      Mizutani S., et al.
    • Journal Title

      Mammalian Genome (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Chromosome 2 locus Nidd5 has a potent effect on adiposity in the TSOD mouse.

    • Author(s)
      Mizutani S., et al.
    • Journal Title

      Mammalian Genome (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Chromosome 2 locus Nidd5 has a potent effect on adiposity in the TSOD mouse.

    • Author(s)
      Mizutani S., et al.
    • Journal Title

      Mammalian Genome (in press)

    • Related Report
      2005 Annual Research Report

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Published: 2004-03-31   Modified: 2016-04-21  

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