DEDIFFERENTIATION OF NON-HEMATOPOIETIC TISSUE BY GENETIC MANIPULATION AND ITS ACQUISITION OF PLASTICITY AND HEMATOPOIETIC TRANSDIFFERENTIATION

• Project/Area Number: 16390281
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Hematology
• Research Institution: JICHI MEDICAL UNIVERSITY
• Principal Investigator: OZAWA Keiya Jichi Medical University, Faculty of Medicine, Professor, 医学部, 教授 (30137707)
• Co-Investigator(Kenkyū-buntansha): KUME Akihiro Jichi Med. Univ., Faculty of Medicine, Associate Professor, 医学部, 助教授 (10264293) ; MIZUKAMI Hiroaki Jichi Med. Univ., Faculty of Medicine, Assistant Professor, 医学部, 講師 (20311938) ; MATSUSITA Takashi Jichi Med. Univ., Faculty of Medicine, Research Associate, 医学部, 助手 (20343444)
• Project Period (FY): 2004 – 2005
• Project Status: Completed (Fiscal Year 2005)
• Budget Amount: ¥12,000,000 (Direct Cost: ¥12,000,000); Fiscal Year 2005: ¥5,600,000 (Direct Cost: ¥5,600,000); Fiscal Year 2004: ¥6,400,000 (Direct Cost: ¥6,400,000)
• Keywords: Dedifferentiation / Transdifferentiation / Plasticity / Msx1 / AAV vector / Skeletal muscle / Hematopoietic system / Stem cell / AVVベクター

Research Abstract

We explored the possibility that genetic manipulation may enhance the efficiency of transdifferentiation of non-hematopoietic tissue. Transient overexpression of Msx1 in muscles was reported to generate abundant mononuclear cells (MNCs) capable of differentiation into myotubes, chondrocytes, adipocytes and osteocytes. Since virtually all of AAV vector-mediated transgenes exist as a non-integrated form, they gradually disappear as the host cells divide. We took advantage of this feature of AAV vectors ; i.e. muscle-derived MNCs lose Msx1 transgenes through multiple cell divisions after dedifferentiation. We postulated that a proper differentiation cue might redirect muscle-derived undifferentiated MNCs into a hematopoietic lineage. AAV5 vector expressing Msx1 (AAV-msx1) was injected into tibialis anterior muscles of C57BL/6 mice. Flow cytometric analysis revealed that MNCs from AAV-msx1-treated muscles contained a considerable number of cells expressing hematopoietic stem cell markers. To evaluate hematopoietic activity, MNCs were cultured in methylcellulose medium. After AAV-msx1 injection, colony-forming cells in the muscles were gradually increased, reaching a peak at 3 wk. In vivo hematopoietic reconstitution activity was also evaluated by transplanting MNCs from AAV-msx1-treated muscles of Ly5.2 mice to irradiated congenic mice. Efficient engraftment of Ly5.2 cells was observed, and these transplants showed a very high chimerism of Ly 5.2. Furthermore, in the secondary bone marrow transplantation from the former mouse to a Ly5.1/5.2 heterozygous recipient, the donor cell chimerism was even higher. These results suggest that enforced Msx1 expression can reprogram muscle cells into multipotential cells capable of differentiation into a hematopoietic lineage as well. This novel technology would be applied to the treatment of acquired bone marrow failure using genetically-normal hematopoietic stem cells derived from patient muscles.

Research Products

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  • Journal Title: Mol. Ther. 13・1 Pages: 160-166
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  • Author(s): Urabe, M., Xin, K.Q., Obara, Y., Nakakura, T., Mizukami, H., Kume, A., Okuda, K., Ozawa, K.
  • Journal Title: Mol.Ther. 13 Pages: 823-828
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] rAAV-mediated shRNA ameliorated neuropathology in Huntington disease model mouse. (2006)
  • Author(s): Machida, Y., Okada, T., Kurosawa, M., Oyama, F., Ozawa, K., Nukina, N.
  • Journal Title: Biochem.Biophys.Res.Commun. 343 Pages: 190-197
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Scalable generation of high-titer recombinant adeno-associated virus type 5 in insect cells. (2006)
  • Author(s): Urabe M, Nakakura T, Xin KQ, Obara Y, Mizukami H, Kume A, Kotin RM, Ozawa K.
  • Journal Title: J.Virol. 80 Pages: 1874-1885
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A histone deacetylase inhibitor enhances recombinant adeno-associated virus-mediated gene expression in tumor cells. (2006)
  • Author(s): Okada, T., Uchibori, R., Iwata-Okada, M., Takahashi, M., Nomoto, T., Nonaka-Sarukawa, M., Ito, T., Liu, Y., Mizukami, H., Kume, A., Kobayashi, E., Ozawa, K.
  • Journal Title: Mol.Ther. 13 Pages: 738-746
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Viral-mediated temporally controlled dopamine production in a rat model of Parkinson disease (2006)
  • Author(s): Li, X.G., Okada, T., Kodera, M., Nara, Y., Takino, N., Muramatsu, C., Ikeguchi, K., Urano, F., Ichinose, H., Metzger, D., Chambon, P., Nakano, I., Ozawa, K., Muramatsu, S.
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• [Journal Article] Repair of articular cartilage defect by intraarticular administration of basic fibroblast growth factor gene, using adeno-associated virus vector. (2005)
  • Author(s): Hiraide, A.
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• [Journal Article] Transforming activity of the lymphotoxin-beta receptor revealed by expression screening. (2005)
  • Author(s): Fujiwara, S.
  • Journal Title: Biochem. Biophys. Res. Commun. 338・2 Pages: 1256-1262
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  • Author(s): Liu, Y.
  • Journal Title: Mol. Ther. 12・4 Pages: 725-733
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  • Journal Title: Proc. Natl. Acad. Sci. U.S.A. 102・27 Pages: 9655-9660
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• [Journal Article] Repair of infarcted myocardium mediated by transplanted bone marrow-derived CD34' stem cells in a nonhuman primate model. (2005)
  • Author(s): Yoshioka, T.
  • Journal Title: Stem Cells 23・3 Pages: 355-364
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Development of new inbred transgenic strains of rats with LacZ or GFP. (2005)
  • Author(s): Inoue, H.
  • Journal Title: Biochem. Biophys. Res. Commun. 329・1 Pages: 288-295
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Hematopoietic microchimerism in sheep after in utero transplantation of cultured cynomolgus embryonic stem cells. (2005)
  • Author(s): Sasaki, K.
  • Journal Title: Transplantation 79・1 Pages: 32-37
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] TEF, an anti-apoptotic bZIP transcription factor related to the oncogenic E2A-HLF chimera, inhibits cell growth by down regulating expression of the common β chain of cytokine receptors. (2005)
  • Author(s): Inukai, T.
  • Journal Title: Blood 105・11 Pages: 4437-4444
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Repair of articular cartilage defect by autologous transplantation of basic fibroblast growth factor gene-transduced chondrocytes with adeno-associated virus vector. (2005)
  • Author(s): Yokoo, N.
  • Journal Title: Arthritis Rheum. 52・1 Pages: 164-170
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• [Journal Article] Efficient and stable Sendai virus-mediated gene transfer into primate embryonic stem cells with pluripotency preserved. (2005)
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  • Journal Title: Gene Ther. 12・3 Pages: 203-210
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  • Author(s): Hiraide, A., Yokoo, N., Xin, K.Q., Okuda, K., Mizukami, H., Ozawa, K., Saito, T.
  • Journal Title: Hum.Gene Ther. 16 Pages: 1413-1421
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Safe and efficient collection of cytokine-mobilized peripheral blood cells from cynomolgus monkeys (Macaca fascicularis) with human newborn-equivalent body weights. (2005)
  • Author(s): Ageyama, N., Hanazono, Y., Shibata, H., Ono, F., Ogawa, H., Nagashima, N., Ueda, Y., Yoshikawa, Y., Hasegawa, M., Ozawa, K., Terao, K.
  • Journal Title: Exp.Anim. 54 Pages: 421-428
  • NAID: 10016785510
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Transforming activity of the lymphotoxin-beta receptor revealed by expression screening. (2005)
  • Author(s): Fujiwara, S., Yamashita, Y., Choin Y.L., Wada, T., Kaneda, R., Takada, S., Maruyama, Y., Ozawa, K., Mano, H.
  • Journal Title: Biochem.Biophys.Res.Commun. 338 Pages: 1256-1262
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Large-scale production of recombinant viruses by use of a large culture vessel with active gassing. (2005)
  • Author(s): Okada, T., Nomoto, T., Yoshioka, T., Nonaka-Sarukawa, M., Ito, T., Ogura, T., Iwata-Okada, M., Uchibori, R., Shimazaki, K., Mizukami, H., Kume, A., Ozawa, K.
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  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Specific and efficient transduction of cochlear inner hair cells with recombinant adeno-associated virus type 3 vector. (2005)
  • Author(s): Liu, Y., Okada, T., Sheykholeslami, K., Shimazaki, K., Nomoto, T., Muramatsu, S., Kanazawa, T., Takeuchi, K., Ajalli, R., Mizukami, H., Kume, A., Ichimura, K., Ozawa, K.
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  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Frag1, a homolog of alternative replication factor C subunits, links replication stress surveillance with apoptosis. (2005)
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• [Journal Article] Repair of infarcted myocardium mediated by transplanted bone marrow-derived CD34^+ stem cells in a nonhuman primate model. (2005)
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  • Journal Title: Stem Cells 23 Pages: 355-364
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Development of new inbred transgenic strains of rats with LacZ or GFP. (2005)
  • Author(s): Inoue, H., Ohsawa, I., Murakami, T., Kimura, A., Hakamata, Y., Sato, Y., Kaneko, T., Takahashi, M., Okada, T., Ozawa, K., Francis, J., Leone, P., Kobayashi, E.
  • Journal Title: Biochem.Biophys.Res.Commun. 329 Pages: 288-295
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Hematopoietic microchimerism in sheep after in utero transplantation of cultured cynomolgus embryonic stem cells. (2005)
  • Author(s): Sasaki, K., Nagao, Y., Kitano, Y., Hasegawa, H., Shibata, H., Takatoku, M., Hayashi, H., Ozawa, K., Hanazono, Y.
  • Journal Title: Transplantation 79 Pages: 32-37
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] TEF, an anti-apoptotic bZIP transcription factor related to the oncogenic E2A-HLF chimera, inhibits cell growth by down regulating expression of the common b chain of cytokine receptors. (2005)
  • Author(s): Inukai, T., Inaba, T., Dang, J., Kuribara, R., Ozawa, K., Miyajima, A., Wu, W., Look, A.T., Arinobu, Y., Iwasaki, H., Akashi, K., Kagami, K., Goi, K., Sugita, K., Nakazawa, S.
  • Journal Title: Blood 105 Pages: 4437-4444
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Repair of articular cartilage defect by autologous transplantation of basic fibroblast growth factor gene-transduced chondrocytes with adeno-associated virus vector. (2005)
  • Author(s): Yokoo, N., Saito, T., Uesugi, M., Kobayashi, N., Xin, K.Q., Okuda, K., Mizukami, H., Ozawa, K., Koshino, T.
  • Journal Title: Arthritis Rheum. 52 Pages: 164-170
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Efficient and stable Sendai virus-mediated gene transfer into primate embryonic stem cells with pluripotency preserved. (2005)
  • Author(s): Sasaki, K., Inoue, M., Shibata, H., Ueda, Y., Muramatsu, S., Okada, T., Hasegawa, M., Ozawa, K., Hanazono, Y.
  • Journal Title: Gene Ther. 12 Pages: 203-210
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Safe and efficient collection of cytokine-mobilized peripheral blood cells from cynomolgus monkeys (Macaca fascicularis) with human newborn-equivalent body weights. (2005)
  • Author(s): Ageyama, N.
  • Journal Title: Exp.Anim. 54・5 Pages: 421-428
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  • Author(s): Fujiwara, S.
  • Journal Title: Biochem.Biophys.Res.Commun. 338・2 Pages: 1256-1262
• [Journal Article] Large-scale production of recombinant viruses by use of a large culture vessel with active gassing. (2005)
  • Author(s): Okada, T.
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• [Journal Article] Repair of infarcted myocardium mediated by transplanted bone marrow-derived CD34^+ stem cells in a nonhuman primate model. (2005)
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  • Journal Title: Stem Cells 23・3 Pages: 355-364
• [Journal Article] Development of new inbred transgenic strains of rats with LacZ or GFP. (2005)
  • Author(s): Inoue, H.
  • Journal Title: Biochem.Biophys.Res.Commun 329・1 Pages: 288-295
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  • Author(s): Yoshioka, T.
  • Journal Title: Stem Cells. 23・3 Pages: 355-364
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  • Journal Title: Gene Ther. Reg. 2・3 Pages: 207-218
  • Description: 「研究成果報告書概要(和文)」より
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  • Author(s): Ueda, K., Hanazono, Y., Ageyama, N., Shibata, H., Ogata, S., Ueda, Y., Tabata, T., Ikehara, S., Taniwaki, M., Hasegawa, M., Terao, K., Ozawa, K.
  • Journal Title: Gene Ther.Reg. 2 Pages: 207-218
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Adeno-associated virus (AAV) vector-mediated liver- and muscle-directed transgene expression using various kinds of promoters and serotypes. (2004)
  • Author(s): Mochizuki, S., Mizukami, H., Kume, A., Muramatsu, S., Takeuchi, K., Matsushita, T., Okada, T., Kobayashi, E., Hoshika, A., Ozawa K.
  • Journal Title: Gene Ther.Mol.Biol. 8 Pages: 9-18
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Adeno-associated virus vector-mediated interleukin-10 gene transfer inhibits atherosclerosis in apolipoprotein E-deficient mice. (2004)
  • Author(s): Yoshioka, T., Okada, T., Maeda, Y., Ikeda, U., Shimpo, M., Nomoto, T., Takeuchi, K., Nonaka-Sarukawa, M., Ito, T., Takahashi, M., Matsushita, T., Mizukami, H., Hanazono, Y., Kume, A., Ookawara, S., Kawano, M., Ishibashi, S., Shimada, K., Ozawa, K.
  • Journal Title: Gene Ther. 11 Pages: 1772-1779
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Sustained transgene expression by human cord blood derived CD34(+) cells transduced with simian immunodeficiency virus agmTYO1-based vectors carrying the human coagulation factor VIII gene in NOD/SCID mice. (2004)
  • Author(s): Kikuchi, J., Mimuro, J., Ogata, K., Tabata, T., Ueda, Y., Ishiwata, A., Kimura, K., Takano, K., Madoiwa, S., Mizukami, H., Hanazono, Y., Kume, A., Hasegawa, M., Ozawa, K., Sakata, Y.
  • Journal Title: J Gene Med. 6 Pages: 1049-1060
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] High-level in vivo gene marking after gene-modified autologous hematopoietic stem cell transplantation without marrow conditioning in nonhuman primates. (2004)
  • Author(s): Ueda, K., Hanazono, Y., Shibata, H., Ageyama, N., Ueda, Y., Ogata, S., Tabata, T., Nagashima, T., Takatoku, M., Kume, A., Ikehara, S., Taniwaki, M., Terao, K., Hasegawa, M., Ozawa, K.
  • Journal Title: Mol.Ther. 10 Pages: 469-477
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Topoisomerase inhibitors enhance the cytocidal effect of AAV-HSVtk/ganciclovir on head and neck cancer cells. (2004)
  • Author(s): Kanazawa, T., Mizukami, H., Nishino, H., Okada, T., Hanazono, Y., Kume, A., Kitamura, K., Ichimura, K., Ozawa, K.
  • Journal Title: Int.J.Oncol. 25 Pages: 729-735
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Expansion of genetically corrected neutrophils in chronic granulomatous disease mice by cotransferring a therapeutic gene and a selective amplifier gene. (2004)
  • Author(s): Hara, T., Kume, A., Hanazono, Y., Mizukami, H., Okada, T., Tsurumi, H., Moriwaki, H., Ueda, Y., Hasegawa, M., Ozawa, K.
  • Journal Title: Gene Ther. 11 Pages: 1370-1377
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Translocation and cleavage of myocardial dystrophin as a common pathway to advanced heart failure : a scheme for the progression of cardiac dysfunction. (2004)
  • Author(s): Toyo-Oka, T., Kawada, T., Nakata, J., Xie, H., Urabe, M., Masui, F., Ebisawa, T., Tezuka, A., Iwasawa, K., Nakajima, T., Uehara, Y., Kumagai, H., Kostin, S., Schaper, J., Nakazawa, M., Ozawa, K.
  • Journal Title: Proc.Natl.Acad.Sci.U.S.A. 101 Pages: 7381-7385
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Roles of Bim in apoptosis of normal and Bcr-Abl-expressing hematopoietic progenitors. (2004)
  • Author(s): Kuribara, R., Honda, H., Matsui, H., Shinjyo, T., Inukai, T., Sugita, K., Nakazawa, S., Hirai, H., Ozawa.K., Inaba, T.
  • Journal Title: Mol.Cell.Biol. 24 Pages: 6172-6183
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The adenovirus E1A and E1B19K genes provide a helper function for transfection-based adeno-associated virus vector production. (2004)
  • Author(s): Matsushita, T., Okada, T., Inaba, T., Mizukami, H., Ozawa, K., Colosi, P.
  • Journal Title: J.Gen.Virol. 85 Pages: 2209-2214
  • NAID: 110006452410
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Successful gene transfer using adeno-associated virus vectors into the kidney : comparison among adeno-associated virus serotype 1 to 5 vectors in vitro and in vivo. (2004)
  • Author(s): Takeda, S., Takahashi, M., Mizukami, H., Kobayashi, E., Takeuchi, K., Hakamata, Y., Kaneko, T., Yamamoto, H., Ito, C., Ozawa, K., Ishibashi, K., Matsuzaki, T., Takata, K., Asano, Y., Kusano, E.
  • Journal Title: Nephron Exp.Nephrol. 96 Pages: 119-126
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Long-term correction of hyperphenylalaninemia by AAV-mediated gene transfer leads to behavioral recovery in phenylketonuria mice. (2004)
  • Author(s): Mochizuki, S., Mizukami, H., Ogura, T., Kure, S., Ichinohe, A., Kojima, K., Matsubara, Y., Kobayashi, E., Okada, T., Hoshika, A., Ozawa, K., Kume, A.
  • Journal Title: Gene Ther. 11 Pages: 1081-1086
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Separate control of Rep and Cap expression utilizing mutant and wild-type loxP sequences and improved packaging system for adeno-associated virus vector production. (2004)
  • Author(s): Mizukami, H., Okada, T., Ogasawara, Y., Matsushita, T., Urabe, M., Kume, A., Ozawa, K.
  • Journal Title: Mol.Biotechnol. 27 Pages: 7-14
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] In situ generation of pseudotyped retroviral progeny by adenovirus-mediated transduction of tumor cells enhances the killing effect of HSV-tk suicide gene therapy in vitro and in vivo. (2004)
  • Author(s): Okada, T., Caplen, N.J., Ramsey, W.J., Onodera, M., Shimazaki, K., Nomoto, T., Ajalli, R., Wildner, O., Morris, J., Kume, A., Hamada, H., Blaese, R.M., Ozawa, K.
  • Journal Title: J.Gene Med. 6 Pages: 288-289
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Presynaptic localization of neprilysin contributes to efficient clearance of amyloid-b peptide in mouse brain. (2004)
  • Author(s): Iwata, N., Mizukami, H., Shirotani, K., Takaki, Y., Muramatsu, S., Lu, B., Gerard, N.P., Gerard, C., Ozawa, K.Saido, T.C.
  • Journal Title: J.Neurosci. 24 Pages: 991-998
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Expression of human coagulation factor VIII in adipocytes transduced with the simian immunodeficiency virus agmTYO1-based vector for hemophilia A gene therapy. (2004)
  • Author(s): Ogata, K., Mimuro, J., Kikuchi, J., Tabata, T., Ueda, Y., Naito, M., Madoiwa, S., Takano, K., Hasegawa, M., Ozawa, K., Sakata, Y.
  • Journal Title: Gene Ther. 11 Pages: 253-259
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] In vivo expansion of gene-modified hematopoietic cells by a novel selective amplifier gene utilizing the erythropoietin receptor as a molecular switch. (2004)
  • Author(s): Nagashima, T., Ueda, Y., Hanazono, Y., Kume, A., Shibata, H., Ageyama, N., Terao, K., Ozawa, K., Hasegawa, M.
  • Journal Title: J.Gene Med. 6 Pages: 22-31
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] High-level in vivo gene marking after gene-modified autologous hematopoietic stem cell transplantation without marrow conditioning in nonhuman primates. (2004)
  • Author(s): Ueda, K.
  • Journal Title: Mol.Ther. 10・3 Pages: 469-477
• [Journal Article] Translocation and cleavage of myocardial dystrophin as a common pathway to advanced heart failure : a scheme for the progression of cardiac dysfunction. (2004)
  • Author(s): Toyo-Oka, T.
  • Journal Title: Proc.Natl.Acad.Sci.U.S.A. 101・19 Pages: 7381-7385
• [Journal Article] The adenovirus E1A and E1B19K genes provide a helper function for transfection-based adeno-associated virus vector production. (2004)
  • Author(s): Matsushita, T.
  • Journal Title: J.Gen.Virol. 85・Pt8 Pages: 2209-2214
  • NAID: 110006452410
• [Journal Article] In vivo expansion of gene-modified hematopoietic cells by a novel selective amplifier gene utilizing the erythropoietin receptor as a molecular switch. (2004)
  • Author(s): Nagashima, T.
  • Journal Title: J.Gene Med. 6・1 Pages: 22-31
• [Journal Article] Separate control of Rep and Cap expression utilizing mutant and wild-type loxP sequences and improved packaging system for adeno-associated virus vector production. (2004)
  • Author(s): Mizukami, H.
  • Journal Title: Mol.Biotechnol. 27・1 Pages: 7-14
• [Journal Article] Adeno-Associated Virus (AAV) vector-mediated liver- and muscle-directed transgene expression using various kinds of promoters and serotypes. (2004)
  • Author(s): Mochizuki, S.
  • Journal Title: Gene Ther.Mol.Biol. 8・1 Pages: 9-18
• [Journal Article] Utility of intraperitoneal administration as a route of AAV serotype 5 vector-mediated neonatal gene transfer.
  • Author(s): Ogura, T.
  • Journal Title: J. Gene Med. (in press)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Utility of intraperitoneal administration as a route of AAV serotype 5 vector-mediated neonatal gene transfer.
  • Author(s): Ogura, T., Mizukami, H., Mimuro, J., Madoiwa, S., Okada, T., Matsushita, T., Urabe, M., Kume, A., Hamada, H., Yoshikawa, H., Sakata, Y., Ozawa, K.
  • Journal Title: J.Gene Med. (in press)
  • Description: 「研究成果報告書概要(欧文)」より