Establishment of therapeutic strategy for degenerative muscular disease by mouse or human somatic stem cells
| Project/Area Number |
16390300
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Kyoto University |
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Principal Investigator |
HEIKE Toshio Kyoto University, Graduate School of Medicine, Associate Professor, 医学研究科, 助教授 (90190173)
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| Co-Investigator(Kenkyū-buntansha) |
YORIFUJI Tohru Kyoto University, Graduate School of Medicine, Lecturer, 医学研究科, 講師 (60220779)
HIRAMATSU Hidefumi Kyoto University, Graduate School of Medicine, Assistant professor, 医学研究科, 助手 (40362503)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥14,300,000 (Direct Cost: ¥14,300,000)
Fiscal Year 2005: ¥6,900,000 (Direct Cost: ¥6,900,000)
Fiscal Year 2004: ¥7,400,000 (Direct Cost: ¥7,400,000)
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| Keywords | skeletal muscle / hematopoietic stem cell / GFP / c-kit / Sca-1 / Lin / satellite cell / muscular stem cell / 多能性幹細胞 / sphere / 筋ジストロフィー症 / 間葉系幹細胞 / 再生 / 機能 |
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| Research Abstract |
The contribution of hematopoietic stem cells into the regeneration of skeletal muscles was investigated. The regenerative pathway of mouse skeletal muscles after the transplantation of GFP-transgenic mouse-derived c-kit+ Sca-1+ Lin- cells along with irradiation was observed precisely. The GFP-positive skeletal muscles was detected 1 week after transplantation. This GFP-positive muscles were replaced by GFP-negative ones gradually and could not be detected thereafter. At 6 months after transplantation, GFP-positive muscles could not be confirmed. However, surprisingly, single muscle fiber culture at 6 months revealed that GFP-positive muscle fibers could be produced and GFP-positive satellite cells (skeletal muscle stem cells) was demonstrated along muscle fibers. These results imply that c-kit+ Sca-1+ Lin- cells contribute to the regeneration of skeletal muscle at not only short terms but also long terms.
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Report
(3 results)
Research Products
(21 results)
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[Journal Article] Generation of pluripotent stem cells from neonatal mouse testis.2004
Author(s)
Kanatsu-Shinohara M., Inoue K., Lee J., Yoshimoto M., Ogonuki N., Miki H., Baba S., Kato T., Kazuki Y., Toyokuni S., Toyoshima M., Niwa O., Oshimura M., Heike T., Nakahata T., Ishino F., Ogura A., Shinohara T.
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Journal Title
Cell 119
Pages: 1001-1012
Description
「研究成果報告書概要(欧文)」より
Related Report
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