Exhaustive analysis about juvenile idiopathic arthritis by using an anti-IL-6 receptor antibody

• Project/Area Number: 16390305
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Yokohama City University
• Principal Investigator: YOKOTA Shumpei Yokohama City University, Medical Research, Professor, 医学研究科, 教授 (10158363)
• Co-Investigator(Kenkyū-buntansha): MORI Masaaki Yokohama City University, Medical Department, Associate professor, 医学部, 準教授 (30254204) ; MITSUDA Toshihiro Yokohama City University, Medical Hospital, Associate professor, 付属病院, 準教授 (20336548) ; NARUTO Takuya Yokohama City University, Medical Department, Researcher, 医学部, 研究員 (60438124) ; 片倉 茂樹 横浜市立大学, 医学部附属病院, 講師 (30347314)
• Project Period (FY): 2004 – 2006
• Project Status: Completed (Fiscal Year 2006)
• Budget Amount: ¥14,400,000 (Direct Cost: ¥14,400,000); Fiscal Year 2006: ¥3,000,000 (Direct Cost: ¥3,000,000); Fiscal Year 2005: ¥3,000,000 (Direct Cost: ¥3,000,000); Fiscal Year 2004: ¥8,400,000 (Direct Cost: ¥8,400,000)
• Keywords: juvenile idiopathic arthritis / IL-6 / anti IL-6 receptor antibody / investigation for pathogenesis / hypercytokine diseases / 若年性特発性関節炎 / プロテオーム解析法 / DNAチップ

Research Abstract

We analyzed the identification of the target molecule of the IL-6 obstruction treatment by using proteomics method such as 2D-DIGE (fluorescent labeling 2 dimension differential gel electrophoresis) and iTRAQ (Isotope Tag for Relative and Absolute Quantitation) method. Additionally, we examined the role of IL-6 on Kawasaki disease or CINCA syndrome as an axis of this research.
Furthermore, we would like to analyze the IL-6 diseases (cytokine-related disease) in pediatric field, and to examine the structure and/or the function of the obstruction target molecule of antiIL-6 receptor antibody treatment front and back in detail in future. Finally, in this study, we detected lots of proteins in sera and plasma of JIA patients using two methods, 2D-DIGE and iTRAQ and could identify some of them.

Research Products

• [Journal Article] Therapeutic Efficacy of Humanized Recombinant Anti-IL 6-receptor Antibody in Children with Systemic-onset Juvenile Idiopathic Arthritis. (2005)
  • Author(s): Shumpei Yokota, Takako Miyamae, Iwata N, Katakura S, Mori M, Woo P, Nishimoto N, Yoshizaki K, Kishimoto T.
  • Journal Title: Arthritis Rheum 52 Pages: 818-825
• [Journal Article] Clinical study of tocilizumab in children with systemic-onset juvenile idiopathic arthritis. (2005)
  • Author(s): Shumpei Yokota, Takako Miyamae, Tomoyuki Imagawa, Shigeki Katakura, Rumiko Kurosawa, Masaaki Mori.
  • Journal Title: Clin Rev Allergy Immunol. 28・3 Pages: 231-238
• [Journal Article] Tocilizumab, Anti-IL-6 Receptor Monoclonal Antibody : Randomized, Double-blind, Placebo-controlled, Withdrawal Phase III Trial of Efficacy and Safety against Systemic-onset Juvenile Idiopathic Arthritis
  • Author(s): Yokota S, Imagawa T.
  • Journal Title: NEJM (in print)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Proposal for Juvenile Idiopathic Arthritis Guidance on Diagnosis and Treatment in Primary Care (2007)
  • Author(s): Yokota S, Mori M.
  • Journal Title: Modern Rheumatology (in print)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Scientific Study on the Occurrence Status of Influenza-associated Symptoms
  • Author(s): Yokota S, Mori M
  • Journal Title: JAMA (under construction)
  • Description: 「研究成果報告書概要(欧文)」より