| Project/Area Number |
16390316
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | Osaka University |
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Principal Investigator |
TAKEDA Junji Osaka University, Center for Advanced Science and Innovation, Professor, 先端科学イノベーションセンター, 教授 (50163407)
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| Co-Investigator(Kenkyū-buntansha) |
TOJO Hiromasa Osaka University, Graduate School of Frontier Biosciences, Associate Professor, 生命機能研究科, 助教授 (90135707)
HASHIMOTO Koji Ehime University, School of Medicine, Professor, 医学部, 教授 (00110784)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥14,300,000 (Direct Cost: ¥14,300,000)
Fiscal Year 2005: ¥6,900,000 (Direct Cost: ¥6,900,000)
Fiscal Year 2004: ¥7,400,000 (Direct Cost: ¥7,400,000)
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| Keywords | sphingolipid / epidermis / SPT / conditional gene targeting / barrier function / mouse / 遺伝子破壊 / Arnt / コンディショナルジーンターゲティング / desaturase |
|---|
| Research Abstract |
Although many scientists predict sphingolipids play important roles in epidermis, its direct evidence is still lacking. To investigate physiological roles of epidermal sphingolipids, we generated a mouse line lacking the first step gene of sphigolipid biosynthesis, SPT, in epidermis. The SPT deficient mice died within 3 weeks after birth. Barrier functions were impaired in the SPT deficient mice. With mass spectrometry analysis sphingolipids bearing three fatty acids were abnormally elevated. These results indicate that de novo synthesis of sphingolipids is important for maintenance of epidermal barrier functions.
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