| Project/Area Number |
16390347
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | National Institute of Radiological Sciences |
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Principal Investigator |
TANADA Shuji National Institute of Radiological Sciences, Dept.of Medical Imaging, Director, 画像医学部, 部長 (40116950)
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| Co-Investigator(Kenkyū-buntansha) |
IRIE Toshiaki National Institute of Radiological Sciences, Dept.of Medical Imaging, Head, 画像医学部分子トレーサー開発室, 室長 (40160072)
ENDO Keigo Gunma University, School of Medicine, Professor, 医学部, 教授 (10115800)
MATOZAKI Takashi Gunma University, Institute for Molecular and Cellular Regulation, Professor, 生体調節研究所, 教授 (80252782)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥7,300,000 (Direct Cost: ¥7,300,000)
Fiscal Year 2005: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2004: ¥4,500,000 (Direct Cost: ¥4,500,000)
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| Keywords | Somatostatin / Radioisotope / Imaging / Targeting |
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| Research Abstract |
As octreotide, an analogue of somatostatin, binds specifically to the somatostatin receptor in the living body, it has a potential for specific targeting therapy against cancer if it could be labeled with cytotoxic radioisotope (RI). The present research was undertaken to study the feasibility to develop internal radiotherapy combined with somatostatin receptor binding technique, using gene engineering technique with the expression of somatostatin receptor on cancer cell membrane in cancer-bearing mice. It was also undertaken to possibly develop a quantitative visualizing method of somatostatin receptors on cancer cell membrane using gamma-emitting RI with which octreotide could be labeled.
The most important part was to obtain optimal gene-recombinant adenovirus which could have the ability to express somatostatin receptor on cancer cell membrane after the infection of the virus to the experimental mice. We introduced Gene-recombinant Adenovirus (AxCA-SSTR2) and tried to produce adenov
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irus vector, namely Adex-HAhSStr2 which was transfected with HA-human somatostatin receptor gene type 2, and to obtain the somatostatin-receptor expressing cancer cells with very high efficiencies. Regrettably, we have not reached the satisfactory goal of this step yet and are thinking further research. We have been scrutinizing the technique to label octreotide with beta or gamma emitting RI with high efficiencies, and have encountered some difficulty to achieve the stable result for labeling octreotide with the specific activity sufficient to perform basic animal experiments. We expect to continue further study for the goal.
Imaging technology is essential for precise visualization of cancer lesions and is very important to evaluate the effect of RI internal radiotherapy. Positron emission tomography has been one the most useful imaging technology for the evaluation of therapy effects and predicting patient prognosis. We have studied the optimal imaging technique for this purpose. We also examined the behavior of beta emitting RI in the body for the preparation of future RI internal radiotherapy. Less
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