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Basic immunological analyses of vaccine using dendritic cells transfected tumor RNA against digestive cancer

Research Project

Project/Area Number 16390367
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General surgery
Research InstitutionWakayama Medical University

Principal Investigator

YAMAUE Hiroki  Wakayama Medical University, Second Department of Surgery, Professor (20191190)

Co-Investigator(Kenkyū-buntansha) IWAHASHI Makoto  WAKAYAMA MEDICAL UNIVERSITY, Second Department of Surgery, Instructor (70244738)
TANI Masaji  WAKAYAMA MEDICAL UNIVERSITY, Second Department of Surgery, Instructor (60236677)
NAKAMURA Masaki  WAKAYAMA MEDICAL UNIVERSITY, Second Department of Surgery, Assistant (80364090)
Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥6,900,000 (Direct Cost: ¥6,900,000)
Fiscal Year 2006: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2005: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2004: ¥4,000,000 (Direct Cost: ¥4,000,000)
KeywordsDC / RNA / GM-CSF / Cancer Vaccine / 樹状細胞 / CT26
Research Abstract

Recently, dendritic cells (DCs) transfected with tumor RNA has been used as a cancer vaccine. The efficacy of a cancer vaccine using DCs transfected tumor RNA was examined. Of particular interest was to determine whether a vaccine using DCs transfected with recrudescent tumor RNA is effective for the treatment of a regrowing tumor after prior immunotherapy. In addition, the usefulness of co-transfection of granulocyte macrophage colony-stimulating factor (GM-CSF) mRNA to augment the DC vaccine was examined. CT26 tumor bearing mice were immunized by the subcutaneous injection with DCs transfected with CT26 mRNA (DC-CT26). The cytotoxic activity against CT26 in mice immunized with DC-CT26 was significantly higher than that in control group (p < 0.001) and was augmented by GM-CSF mRNA co-transfection (p < 0.05), resulting in remarkable therapeutic efficacy in CT26 subcutaneous tumor models. CTL induced by the vaccination using DCs transfected with mRNA from the recrudescent tumor showed a potent cytotoxicity against the recrudescent CT26 tumor cells, which was significantly higher than the cytotoxity induced by the vaccination using DC-CT26 (p < 0.05). In addition, in a recrudescent tumor model, this vaccination suppressed the regrowing subcutaneous tumors, and was augmented by GM-CSF mRNA co-transfection (p < 0.05). These results suggested that vaccination therapy using DCs simultaneously transfected with, whole tumor RNA and GM-CSF mRNA could generate a therapeutic immune responses even against recrudescent tumor after prior vaccination.

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (21 results)

All 2008 2007 2006 2005 2004

All Journal Article (21 results)

  • [Journal Article] Tumor vaccine therapy against recrudescent tumor using den dritic cells simultaneously transfected with tumor RNA and granulocyte macrophage colony-stimulating factor RNA2008

    • Author(s)
      Naka T
    • Journal Title

      Cancer Science 99

      Pages: 407-413

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Tumor vaccine therapy against recrudescent tumor using dendritic cells simultaneously transfected with tumor RNA and granulocyte macrophage colony-stimulating factor RNA.2008

    • Author(s)
      Naka T, Iwahashi M, Nakamura M, Ojima T, Nakamori M, Ueda. K, Katsuda M, MiyazawaM, Ishida K, Yamaue H.
    • Journal Title

      Cancer Sci 99

      Pages: 407-413

    • NAID

      10024002848

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Benefits of gene transduction of granulocyte macrophage colony-stimulating factor in cancer vaccine using genetically modified dendritic cells2007

    • Author(s)
      Ojima T
    • Journal Title

      International Journal of Oncology 31

      Pages: 931-939

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Successful cancer vaccine therapy for carcinoembryonic antigen(CEA)-expressing colon cancer using genetically modified dendritic cells that express CEA and T helper-type 1 cytokines in CEA transgenic mice2007

    • Author(s)
      Ojima T
    • Journal Title

      International Journal of Cancer 120(3)

      Pages: 585-593

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Successful cancer vaccine therapy for carcinoembryonic antigen (CEA)-expressing colon cancer using genetically modified dendritic cells that express CEA and T helper-type 1 cytokines in CEA transgenic mice.2007

    • Author(s)
      Ojima T et al.
    • Journal Title

      Int J Cancer 120.3

      Pages: 585-593

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Effect of normothermic perfusion using fructose-1, 6-bisphosphate for maintenance of liver function during in situ extended hepatectomy by the total hepatic vascular exclusion technique.2007

    • Author(s)
      Uemura R et al.
    • Journal Title

      J Surg Res 137.1

      Pages: 89-95

    • Related Report
      2006 Annual Research Report
  • [Journal Article] The boosting effect of co-transduction with cytokine genes on cancer vaccine therapy using genetically modified dendrite cells expressing tumor-associated antigen2006

    • Author(s)
      Ojima T
    • Journal Title

      International Journal of Oncology 28(4)

      Pages: 947-953

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Surgical management of small gastrointestinal stromal tumors of the stomach2006

    • Author(s)
      Iwahashi M
    • Journal Title

      World Journal of Surgery 30(1)

      Pages: 28-35

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Early removal of prophylactic drains reduces the risk of intra-abdominal infections in patients with pancreatic head resection : prospective study for 104 consecutive patients.2006

    • Author(s)
      Kawai M et al.
    • Journal Title

      Ann Surg 244.1

      Pages: 1-7

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Does postoperative chemotherapy have a survival benefit for patients with pancreatic cancer?2006

    • Author(s)
      Tani M et al.
    • Journal Title

      J Surg Oncol 93.6

      Pages: 485-490

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Improvement of delayed gastric emptying in pylorus-preserving pancreaticoduodenectomy : results of a prospective, randomized, controlled trial.2006

    • Author(s)
      Tani M et al.
    • Journal Title

      Ann Surg 243.3

      Pages: 316-320

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Combination gene therapy of HGF and truncated type II TGF-beta receptor for rat liver cirrhosis after partial hepatectomy.2006

    • Author(s)
      Ozawa S et al.
    • Journal Title

      Surgery 139.4

      Pages: 563-573

    • Related Report
      2006 Annual Research Report
  • [Journal Article] The boosting effect of co-transduction with cytokine genes on cancer vaccine therapy using genetically modified dendrite cells expressing tumor-associated atigen2006

    • Author(s)
      Ojima T et al.
    • Journal Title

      Int J Oncol 28(4)

      Pages: 947-953

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Combination gene therapy of HGF and truncated type II TGF-β receptor for rat liver cirrhosis after partial hepatectomy2006

    • Author(s)
      Ozawa S et al.
    • Journal Title

      Surgery (In press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Dendritic cells transduced with tumor-associated antigen gene elicit potent therapeutic antitumor immunity:comparison with immunodominant peptide-pulsed DCs2005

    • Author(s)
      Nakamura M
    • Journal Title

      Oncology 68(2-3)

      Pages: 163-170

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Dendritic cells transduced with tumor-associated antigen gene elicit potent therapeutic antitumor immunity : comparison with immunodominant peptide-pulsed DCs2005

    • Author(s)
      Nakamura M et al.
    • Journal Title

      Oncology 68(2-3)

      Pages: 163-170

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Individualized adjuvant chemotherapy guided by chemosensitivity test sequential to extended surgery for advanced gastric cancer2005

    • Author(s)
      Iwahashi M et al.
    • Journal Title

      Anticancer Res 25(5)

      Pages: 3453-3459

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Evaluation of chemosensitivity testing with highly purified tumor cells i 435 patients with gasytric cancer using an MTT assay2005

    • Author(s)
      Noguchi K et al.
    • Journal Title

      Anticancer Res 25(2A)

      Pages: 931-937

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Dendritic cells transduced with tumor-associated antigen gene elicit potent therapeutic antitumor immunity : comparison with immunodominant peptide-pulsed DCs2005

    • Author(s)
      Nakamura M
    • Journal Title

      Oncology in press

    • Related Report
      2004 Annual Research Report
  • [Journal Article] T-lymphocyte responses in patients with gastrointestinal malignancies following vaccination with CEA peptide-pulseddenciritic cells.2004

    • Author(s)
      Matsuda K
    • Journal Title

      Cancer Immunol Immunother 53(7)

      Pages: 609-616

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Adenoviral-mediated Gene transduction of the hepatocyte growth factor (HGF) antagonist, NK4, suppresses peritoneal metastases of gastric cancer in nude mice.2004

    • Author(s)
      Ueda K
    • Journal Title

      Eur J Cancer 40(14)

      Pages: 2135-2142

    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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