New SEREX Antigens, Molecular Target to Diagnosis and Treatment for Esophageal Cancer

• Project/Area Number: 16390372
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Chiba University
• Principal Investigator: OCHIAI Takenori Chiba University, GRADUATE SCHOOL OF MEDICINE, PROFESSOR, 大学院・医学研究院, 教授 (80114255)
• Co-Investigator(Kenkyū-buntansha): SHIMADA Hideaki Chiba University, GRADUATE SCHOOL OF MEDICINE, ASSISTANT PROFESSO, 大学院・医学研究院, 講師 (20292691) ; HIWASA Takaki Chiba University, GRADUATE SCHOOL OF MEDICINE, ASSOCIATE PROFESSOR, 大学院・医学研究院, 助教授 (30260251) ; TOMONAGA Takashi Chiba University, GRADUATE SCHOOL OF MEDICINE, ASSOCIATE PROFESSOR, 大学院・医学研究院, 助教授 (80227644)
• Project Period (FY): 2004 – 2006
• Project Status: Completed (Fiscal Year 2006)
• Budget Amount: ¥14,100,000 (Direct Cost: ¥14,100,000); Fiscal Year 2006: ¥4,100,000 (Direct Cost: ¥4,100,000); Fiscal Year 2005: ¥4,100,000 (Direct Cost: ¥4,100,000); Fiscal Year 2004: ¥5,900,000 (Direct Cost: ¥5,900,000)
• Keywords: Esophageal cancer / Squamous cell carcinoma / SEREX / tumor marker / TROP2 / GLUT-1 / SLC2A1 / Myomegalin / TRIM21 / GLUT1 / myomegalin / 食道扁平上皮癌 / SEREX抗体

Research Abstract

In order to identify new serum markers of esophageal squamous cell carcinoma (SCC), we performed serological identification of antigens by recombinant cDNA expression cloning (SEREX). E.coli was infected with λZAPII phage cDNA library prepared from mRNA of an esophageal cancer cell line (T.Tn), and IPTG-induced cDNA products were screened for interaction with antibodies in allogeneic sera of patients with esophageal SCC. We identified TROP-2, GLUT-1/SLC2A1, TRIM21, myomegalin, as new SEREX antigens for esophageal SCC. Western blotting analysis revealed that serum anti-antibodies for these SEREX antigens were present in 20 to 47% of patients but not in healthy controls. Although the presence of serum SEREX-Abs were not related to any of clinicopathological features of the patients concerned, multivariate analysis indicated that presence of s-myomegalin-Abs was significantly associated with favorable prognosis. Moreover, s-TROP-2-Abs was associated with tumor size. Because there serum antibodies against SEREX antigens were not associated with other conventional tumor markers, there serum markers were useful as a new tumor markers for esophageal SCC.

Research Products

• [Journal Article] Serum anti-myomegalin antibodies in patients with esophageal squamous cell carcinoma (2007)
  • Author(s): Shimada H., Kuboshima M., Shiratori T., Nabeya Y., Takeuchi A., Takagi H., Nomura, F., Takiguchi, M., Ochiai, T., Hiwasa, T
  • Journal Title: International Journal of Oncology 30 Pages: 97-103
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] TSerum anti-myomegalin antibodies in patients with esophageal squamous c ell carcinoma (2007)
  • Author(s): Shimada H., Kuboshima M., Shiratori T., Nabeya Y., Takeuchi A., Takagi H., Nomura, F., Takiguchi, M., Ochiai, T.Hiwasa
  • Journal Title: International Journal of Oncology 30 Pages: 97-103
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Serological identification of antigens by recombinant cDNA expression cloning (SEREX) using antibodies from patients with esophageal squamous cell carcinoma (2006)
  • Author(s): Hiwasa, T., Shimada, H., Ochiai, T., Takiguchi
  • Journal Title: Moleculomics and Thereafte Pages: 99-117
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Presence of serum tripartite motif-containing 21 antibodies in patients with esophageal squamous cell carcinoma (2006)
  • Author(s): Kuboshima, M., Shimada, H., Liu, T.L., Nomura, F., Takiguchi, M., Hiwasa, T., Ochiai, T
  • Journal Title: Cancer Science 97 Pages: 380-386
  • NAID: 10018176867
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Enhancement of chemosensitivity toward peplomycin by calpastatin-stabilized NF-kappaB p65 in esophageal carcinoma cells : possible involvement of Fas/Fas-L synergism. (2006)
  • Author(s): Liu, T.L., Shimada, H., Ochiai, T., Shiratori, T., Lin, S.E., Kitagawa, M., Harigaya, K., Maki, M., Oka, M., Abe, T., Takiguchi, M., Hiwasa, T.
  • Journal Title: Apoptosis 11 Pages: 1025-1037
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] An essential role of alternative splicing ofc-myc suppressor FUSE-binding protein-interacting repressor in carcinogenesis. (2006)
  • Author(s): Matsushita, K., Tomonaga, T., Shimada, H., Shioya, A., Higashi, M., Matsubara, H., Harigaya, K., Nomura, F., Libutti, D., Levens, D., Ochiai, T
  • Journal Title: Cancer Res 66 Pages: 1409-1417
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Proteomic analysis of primary esophageal squamous cell carcinoma reveals downregulation of a cell adhesion protein, periplakin. (2006)
  • Author(s): Nishimori, T., Tomonaga, T., Matsushita, K., Oh-Ishi, M., Kodera, Y., Maeda, T., Nomura, F., Matsubara, H., Shimada, H., Ochiai
  • Journal Title: Proteomics 6 Pages: 1011-1018
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Enhancement of chemosensitivity toward peplomycin by calpastatin-stabilized NF-kappaB p65 in esophageal carcinoma cells : possible involvement of Fas/Fas-L synergism. (2006)
  • Author(s): Liu, T.L., Shimada, H., Ochiai, T., Shiratori, T., Lin, S.E., Kitagawa, M., Harigaya, K., Maid, M., Oka, M., Abe, T., Takiguchi, M., Hiwasa, T.
  • Journal Title: Apoptosis 11 Pages: 1025-1037
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] An essential role of alternative splicing ofc-myc suppressor FUSE-binding protein-interacting repressor in carcinogenesis. (2006)
  • Author(s): Matsushita, K., Tomonaga, T., Shimada, H., Shioya, A., Higashi, M., Matsubara, H., Harigaya, K., Nomura, F., Libutti, D., Levens, D.Ochiai, T
  • Journal Title: Cancer Res 66 Pages: 1409-1417
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Proteomic analysis of primary esophageal squamous cell carcino ma revealsdownregulation of a cell adhesion protein, periplakin. (2006)
  • Author(s): Nishimori, T., Tomonaga, T., Matsushita, K., Oh-Ishi, M., Kodera, Y., Maeda, T., Nomura, F., Matsubara, H., Shimada, H., Ochiai
  • Journal Title: Proteomics 6 Pages: 1011-1048
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Enhancement of chemosensitivity toward peplomycin by calpastatin-stabillized NF-kappaB p65 in esophageal carcinoma cells : possible involvement of Fas/Fas-L synergism. (2006)
  • Author(s): Liu, T.L., Shimada, H., Ochiai, T., Shiratori, T., Lin, S.E., Kitagawa, M., Harigaya, K., Maki, M., Oka, M., Abe, T., Takiguchi, M., Hiwasa, T.
  • Journal Title: Apoptosis 11 Pages: 1025-1037
• [Journal Article] An essential role of alternative splicing of c-myc suppressor FUSE-blinding protein-interacting repressor in carcinogenesis. (2006)
  • Author(s): Matsushita, K., Tomonaga, T., Shimada, H., Shioya, A., Higashi, M., Matsubara, H., Harigaya, K., Nomura, F., Libutti, D., Levens, D., Ochiai, T
  • Journal Title: Cancer Res 66 Pages: 1409-1417
• [Journal Article] An essential role of alternative splicing of c-myc suppressor FUSE-binding protein-interacting repressor in carcinogenesis. (2006)
  • Author(s): Matsushita K, Tomonaga T, Shimada H, Shioya A, Higashi M, Matsubara H, Harigaya K, Nomura F, Libutti D, Levens D, Ochiai T.
  • Journal Title: Cancer Res. 66(3) Pages: 1409-1417
• [Journal Article] Proteomic analysis of primary esophageal squamous cell carcinoma reveals down regulation of a cell adhesion protein, periplakin. (2006)
  • Author(s): Nishimori T, Tomonaga T, Matsushita K, Oh-Ishi M, Kodera Y, Maeda T, Nomura F, Matsubara H, Shimada H, Ochiai T.
  • Journal Title: Proteomics 6(3) Pages: 1011-1018
• [Journal Article] Identification of a novel SEREX antigen, SLC2A1/GLUT1, in esophageal squamous cell carcinoma. (2006)
  • Author(s): Kuboshima M, Shimada H, Liu TL, Nakashima K, Nomura F, Takiguchi M, Hiwasa T, Ochiai T.
  • Journal Title: Int J Oncol. 28(2) Pages: 463-468
• [Journal Article] Serological identification of tumor antigens of esophageal squamous cell carcinoma. (2005)
  • Author(s): Shimada H, Nakashima K, Ochiai T, Nabeya Y, Takiguchi M, Nomura F, Hiwasa T.
  • Journal Title: International Journal of Oncology 26 Pages: 77-86
  • NAID: 130004436321
• [Journal Article] Serological identification of TROP2 by recombinant cDNA expression cloning using sera of patients with esophageal squamous cell carcinoma. (2004)
  • Author(s): Nakashima K, Shimada H, Ochiai T, Kuboshima M, Kuroiwa N, Okazumi S, et al.
  • Journal Title: International Journal of Cancer 112 Pages: 1029-1035