The establishment of methods for cardiomyocyte differentiation with human umbilical cord blood-derived mesenchymal stem cells aimed the application into the treatment of pediatric heart failures

• Project/Area Number: 16390399
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Thoracic surgery
• Research Institution: Keio University
• Principal Investigator: AEBA Ryo Keio University, School of Medicine, Assistant Professor (70184025)
• Co-Investigator(Kenkyū-buntansha): UMEZAWA Akihiro National Research Institute for Child Health and Development, Department of Reproductive Biology and Pathology, Director (70213486)
• Project Period (FY): 2004 – 2007
• Project Status: Completed (Fiscal Year 2007)
• Budget Amount: ¥17,400,000 (Direct Cost: ¥16,200,000、Indirect Cost: ¥1,200,000); Fiscal Year 2007: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2006: ¥4,000,000 (Direct Cost: ¥4,000,000); Fiscal Year 2005: ¥4,000,000 (Direct Cost: ¥4,000,000); Fiscal Year 2004: ¥4,200,000 (Direct Cost: ¥4,200,000)
• Keywords: cell transplantation / pediatric severe heart failure / human umbilical cord blood-derived mesenchymal stem cells / 再生医学 / 心筋再生 / 幹細胞 / 臍帯血 / 網羅的遺伝子解析 / 間葉系細胞 / 不死化 / 寿命延長 / 間葉系幹細胞 / 細胞移植治療

Research Abstract

The cell transplantation is a promising strategy to improve impaired organ function with injection of ex vivo cultured cells or media containing growth factors. Major advances have been made in the treatment of heart failures. However, pediatric patients suffering from heart disease is still awaiting major treatment which substitute for heart transplantation, because of several reasons such as the shortage of donor, the requirement for retransplantation, and the economic cost. Cell transplantation would have the advantage on this point, if effectiveness of therapy could be proved. Severe heart failures in childhood with variety of causes, including postoperative myocardial insufficiency, cardiovascular failure, and idiopathic dilated cardiomyopathy, are intractable and remain clinical task. Especially in Japan, it is crucial problem that cardiac transplantation in childhood has not been accepted.
Thus, we focused on cardiomyocytes transplantation for pediatric patients with severe heart failure. Especially, cardiomyocytes cultured ex vivo were studied for the actual clinical application of cell transplantation. The study aims the clinical application of cell transplantation. Collected mesenchymal stem cells were analyzed for gene expression by Gene chip analysis and Western blot analysis.
This study also aimed to exploit the method of cultivation or analysis of human umbilical cord blood-derived mesenchymal stem cells for safe and effective regenerative medicine. In addition, it is. necessary for cell transplantation to generate a care system for recipients in future.

Research Products

• [Journal Article] A case report of surgical correction for congenital mitral regurgitation with subvalvular apparatus abnormality (2008)
  • Author(s): Kudo M, Yozu R, AEBA R, Kokaji K, Kimura N, Iwanaga S.
  • Journal Title: Gen Thorac Cardiovasc Surg 56 Pages: 36-38
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] A case report of surgical correction for congenital mitral regurgitation with subvalvular apparatus abnormality (2008)
  • Author(s): Kudo M, Yozu R, AEBA R, Kokaji K, Kimura N, Iwanaga S
  • Journal Title: Gen Thorac Cardiovasc Surg 56 Pages: 36-38
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A case report of surgical correction for congenital mitral regurgitation with subvalvular apparatus abnormality. (2008)
  • Author(s): Kudo M, Yozu R, AEBA R, Kokaji K, Kimura N, Iwanaga S.
  • Journal Title: Gen Thorac Cardiovasc Surg 56 Pages: 36-38
  • Peer Reviewed
• [Journal Article] Ross手術後の中期遠隔期の心機能-機械弁置換術との比較 (2007)
  • Author(s): 饗庭 了, editorial comment, 成田 純任, 石川 司朗, 石川 友一, 中村 真, 牛ノ濱 大也, 佐川 浩一, 総崎 直樹, 中野 俊秀, 角秀 秋
  • Journal Title: 心臓 39 Pages: 1063-1063
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Chromosomal instability in human mesenchymal stem cells immortalized with human papilloma virus E6, E7, and hTERT genes (2007)
  • Author(s): Takeuchi M, Takeuchi K, Kohara A, Satoh M, Shioda S, Ozawa Y, Ohtani A, Morita K, Hirano T, Terai M, Umezawa A, Mizusawa H.
  • Journal Title: In Vitro Cell Dev Biol Anim 43(3-4) Pages: 129-138
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] A role of Wnt/beta-catenin signals in hepatic fate specification of human umbilical cord blood-derived mesenchymal stem cells (2007)
  • Author(s): Yoshida Y, Shimomura T, Sakabe T, Ishii K, Gonda K, Matsuoka S, Watanabe Y, Takubo K, Tsuchiya H, Hoshikawa Y, Kurimasa A, Hisatome I, Uyama T, Terai M Umezawa A, Shiota G.
  • Journal Title: Am J Physiol Gastrointest Liver Physiol 293(5)
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Preferential localization of SSEA-4 in interfaces between blastomeres of mouse preimplantaion embryos (2007)
  • Author(s): Sato B, Katagiri Y, Miyado K, Akutsu H, Miyashita Y, Horiuchi Y, Nakajima H, Okita H, Umezawa A, Hata J, Fujimoto J, Toshimori K, Kiyokawa N.
  • Journal Title: Biochem Biophys Res Commun 364 Pages: 838-843
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Eye-open at birth phenotype with reduced keratinocyte motility in LGR4 null mice (2007)
  • Author(s): Kato S, Mohri Y, Matsuo T, Ogawa E, Umezawa A, Okuyama R, Nishimori K.
  • Journal Title: FEBS Lett 581(24) Pages: 4685-4690
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Study on the quality control of cell therapy products.Determination of N-glycolylyleuraminic acid incorporated into human cells by nano-flow liquid chromatography/Fourier transformation ion cyclotron mass spectrometry (2007)
  • Author(s): HashiiN, KawasakiN, NakajimaY, Toyoda M, Katagiri Y, ItohS, Harazono A, Umezawa A, Yamaguchi T.
  • Journal Title: J Chromatogr A 1160(1-2) Pages: 263-269
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Ways for a mesenchymal stem cell to live on its own:maintaining an undifferentiated state ex vivo (2007)
  • Author(s): Toyoda M, Takahashi H, Umezawa A.
  • Journal Title: Int J Hematol 86(1) Pages: 1-4
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Hepatic differentiation of human bone marrow-derived UE7T-13cells:Effects of cytokines and CCN family gene expression (2007)
  • Author(s): Shimomura T, Yoshida Y, Sakabe T, Ishii K, Gonda K, Murai R, Takubo K, Tsuchiya H, Hoshikawa Y, Kurimasa A, Hisatome I, Uyama T, Umezawa A, Shiota G.
  • Journal Title: Hepatol Res 37(12) Pages: 1068-1079
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] The Significant Cardiomyogenic Potential of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells in Vitro (2007)
  • Author(s): Nishiyama N, Miyoshi S, Hida N Miss, Uyama T, Okamoto K, Ikegami Y, Miyado K, Segawa K, Terai M, Sakamoto M, Ogawa S, Umezawa A.
  • Journal Title: Stem cells 25(8) Pages: 2017-2024
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Human dystrophin expression in the mdx mouse, a model of Duchenne muscular dystrophy, can be conferred predominantly by "cell fusion" with human menstrual blood-derived cells (2007)
  • Author(s): Cui CH, Uyama T, Miyado K, Terai M, Kyo S, Kiyono T, and Umezawa A.
  • Journal Title: Mol Biol Cell 18(5) Pages: 1586-1594
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Two MSCs : Marrow stromal cells and mesencymal stemcells (2007)
  • Author(s): Umezawa A, Toyoda M.
  • Journal Title: Inflammation and Regeneration 27(1) Pages: 28-36
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Single-cell-derived mesenchymal stem cells overexpressing Csx/Nkx2.5 and GATA4 undergo the stochastic cardiomyogenic fate and behave like transient amplifying cells (2007)
  • Author(s): Yamada Y, Sakurada K, Takeda Y, Gojo S, Umezawa A.
  • Journal Title: Exp Cell Res 313 Pages: 698-706
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Hyaline cartilage formation and enchondral ossification modeled with KUMS and OP9 chondroblasts (2007)
  • Author(s): Sugiki T, Uyama T, Toyoda M, Morioka H, Kume S, Miyado K, Matsumoto K, Saito H, Tsumaki N, Takahashi Y, Toyama Y, Umezawa A.
  • Journal Title: J Cell Biochem 100(5) Pages: 1240-1254
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] 'Working'cardiomyocytes exhibiting plateau action potentials from human placenta-derived extraembryonic mesodermal cells (2007)
  • Author(s): Okamoto K, Miyoshi S, Toyoda M, Hida N, Ikegami Y, Makino H, Nishiyama N, Tsuji H, Cui CH, Segawa K, Uyama T, Kami D, Miyado K, Asada H, M atsumoto K, Saito H, Yoshimura Y, Ogawa S, AEBA R, Yozu R, Umezawa A.
  • Journal Title: Exp Cell Res 313 Pages: 2550-2562
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Aortic translocation with autologous tissue (2007)
  • Author(s): AEBA R, Yozu R
  • Journal Title: Tex Heart Inst J 34 Pages: 420-422
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Midterm cardiopulmonary profile of Ross procedure-Comparison with mechanical valve replacement (2007)
  • Author(s): AEBA, R
  • Journal Title: HEART'S Origina 39(12) Pages: 1063-1063
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Chromosomal instability in human mesenchymal stem cells immortalized with human papilloma virus E6, E7, and hTERT genes (2007)
  • Author(s): Takeuchi, M, Takeuchi, K, Kohara, A, Satoh, M, Shioda, S, Ozawa, Y, Ohtani, A, Morita, K, Hirano, T, Terai, M, Umezawa, A, Mizusawa, H
  • Journal Title: In Vitro Cell Dev Biol Anim 43(3-4) Pages: 129-138
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A role of Wnt/beta-catenin signals in hepatic fate specification of human umbilical cord blood-derived mesenchymal stem cells (2007)
  • Author(s): Yoshida, Y, Shimomura, T, Sakabe, T, Ishii, K, Gonda, K, Matsuoka, S, Watanabe, Y, Takubo, K, Tsuchiya, H, Hoshikawa, Y, Kurimasa, A, Hisatome, I, Uyama, T, Terai, M, Umezawa, A, Shiota, G
  • Journal Title: Am J Physiol Gastrointest Liver Physiol 293(5)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Preferential localization of SSEA-4 in interfaces between blastomeres of mouse preimplantaion embryos (2007)
  • Author(s): Sato, B, Katagiri, Y, Miyado, K, Akutsu, H, Miyashita, Y, Horiuchi, Y, Nakajima, H, Okita, H, Umezawa, A, Hata, J, Fujimoto, J, Toshimori, K, Kiyokawa, N
  • Journal Title: Biochem Biophys Res Commun 364 Pages: 838-843
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Eye-open at birth phenotype with reduced keratinocyte motility in LGR4 null mice (2007)
  • Author(s): Kato, S, Mohri, Y, Matsuo, T, Ogawa, E, Umezawa, A, Okuyama, R, Nishimori, K
  • Journal Title: FEBS Lett 581(24) Pages: 4685-4690
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Study on the quality control of cell therapy products. Determination of N-glycolylneuraminic acid incorporated into human cells by nano-flow liquid chromatography/Fourier transformation ion cyclotron mass spectrometry (2007)
  • Author(s): Hashii, N, Kawasaki, N, Nakajima, Y, Toyoda, M, Katagiri, Y, Itoh, S, Harazono, A, Umezawa, A, Yamaguchi, T
  • Journal Title: J Chromatogr A 1160(1-2) Pages: 263-269
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Ways for a mesenchymal stem cell to live on its own : maintaining an undifferentiated state ex vivo (2007)
  • Author(s): Toyoda, M, Takahashi, H, Umezawa, A
  • Journal Title: Int J Hematol 86(1) Pages: 1-4
  • NAID: 10018515320
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Hepatic differentiation of human bone marrow-derived UE7T-13 cells : Effects of cytokines and CCN family gene expression (2007)
  • Author(s): Shimomura, T, Yoshida, Y, Sakabe, T, Ishii, K, Gonda, K, Murai, R, Takubo, K, Tsuchiya, H, Hoshikawa, Y, Kurimasa, A, Hisatome, I, Uyama, T, Umezawa, A, Shiota, G
  • Journal Title: Hepatol Res 37(12) Pages: 1068-1079
  • NAID: 10019796484
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The Significant Cardiomyogenic Potential of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells in Vitro (2007)
  • Author(s): Nishiyama, N, Miyoshi, S, Hida, N Miss, Uyama, T, Okamoto, K, Ikegami, Y, Miyado, K, Segawa, K, Terai, M, Sakamoto, M, Ogawa, S, Umezawa, A
  • Journal Title: Stem cells 25(8) Pages: 2017-2024
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Human dystrophin expression in the mdx mouse, a model of Duchenne muscular dystrophy, can be conferred predominantly by "cell fusion" with human menstrual blood-derived cells (2007)
  • Author(s): Cui, CH, Uyama, T, Miyado, K, Terai, M, Kyo, S, Kiyono, T, Umezawa, A
  • Journal Title: Mol Biol Cell 18(5) Pages: 1586-1594
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Two MSCs : Marrow stromal cells and mesencymal stemcells (2007)
  • Author(s): Umezawa, A, Toyoda, M
  • Journal Title: Inflammation and Regeneration 27(1) Pages: 28-36
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Single-cell-derived mesenchymal stem cells overexpressing Csx/Nk2.5 and GATA4 undergo the stochastic cardiomyogenic fate and behave like transient amplifying cells (2007)
  • Author(s): Yamada, Y, Sakurada, K, Takeda, Y, Gojo, S, Umezawa, A
  • Journal Title: Exp Cell Res 313 Pages: 698-706
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Hyaline cartilage formation and enchondral ossification modeled with KUM5 and OP9 chondroblasts (2007)
  • Author(s): Sugiki, T, Uyama, T, Toyoda, M, Morioka, H, Kume, S, Miyado, K, Matsumoto, K, Saito, H, Tsumaki, N, Takahashi, Y, Toyama, Y, Umezawa, A
  • Journal Title: J Cell Biochem 100(5) Pages: 1240-1254
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Working" cardiomyocytes exhibiting plateau action potentials from human placenta-derived extraembryonic mesodermal cells (2007)
  • Author(s): Okamoto, K, Miyoshi, S, Toyoda, M, Hida, N, Ikegami, Y, Makino, H, Nishiyama, N, Tsuji, H, Cui, CH, Segawa, K, Uyama, T, Kami, D, Miyado, K, Asada, H, Matsumoto, K, Saito, H, Yoshimura, Y, Ogawa, S, Aeba, R, Yozu, R, Umezawa, A
  • Journal Title: Exp Cell Res 313(12) Pages: 2550-2562
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Aortic translocation with autologous tissue (2007)
  • Author(s): AEBA, R, Yozu, R
  • Journal Title: Tex Heart Inst J 34 Pages: 420-422
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Ross手術後の中期遠隔期の心機能-機械弁置換術との比較 (2007)
  • Author(s): 饗庭 了.editorial comment.成田 純任, 石川 司朗, 石川 友一, 中村 真, 牛ノ濱 大也, 佐川 浩一, 總崎 直樹, 中野 俊秀, 角秀 秋.
  • Journal Title: 心臓 39 Pages: 1063-1063
• [Journal Article] 'Working'cardiomyocytes exhibiting plateau action potentials from human placenta-derived extraembryonic mesodermal cells. (2007)
  • Author(s): Okamoto K, Miyoshi S, Toyoda M, Hida N, Ikegami Y, Makino H, Nishiyama N, Tsuji H, Cui CH, Segawa K, Uyama T, Kami D, Miyado K, Asada H, Matsumoto K, Saito H, Ybshimura Y, Ogawa S, AEBA R, Yozu R, Umezawa A.
  • Journal Title: Exp Cell Res 313 Pages: 2550-2562
  • Peer Reviewed
• [Journal Article] Aortic translocation with autologous tissue. (2007)
  • Author(s): AEBA R, Yozu R.
  • Journal Title: Tex Heart Ihst J 34 Pages: 420-422
  • Peer Reviewed
• [Journal Article] Single-cell-derived mesenchymal stem cells overexpressing Csx/Nkx2.5 and GATA4 undergo the stochastic cardiomyogenic fate and behave like transient amplifying cells. (2007)
  • Author(s): Yamada Y, Sakurada K, Takeda Y, Gojo S, Umezawa A.
  • Journal Title: Exp Cell Res 313 Pages: 698-706
• [Journal Article] A comparison of neural differentiation and retinal transplantation with bone marrow-derived cells and retinal progenitor cells (2006)
  • Author(s): Tomita M, Mori T, Maruyama K, Zahir T, Ward M, Umezawa A, Young MJ.
  • Journal Title: Stem Cells 24(10) Pages: 2270-2278
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Leucine-rich repeat-containing G protein-coupled receptor-4 (LGR4, Gpr48) is essential for renal development in mice (2006)
  • Author(s): Kato S, Matsubara M, Matsuo T, Mohri Y, Kazama I, Hatano R, Umezawa A, Nishimori K.
  • Journal Title: Nephron Exp Nephrol 104(2)
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Differentiation of adult stem cells derived from bone marrow stroma into Leydig or adrenocortical cells (2006)
  • Author(s): Yazawa T, Mizutani T, Yamada K, Kawata H, Sekiguchi T, Yoshino M, Kajitani T, Shou Z, Umezawa A, Miyamoto K.
  • Journal Title: Endocrinology 147(9) Pages: 4104-4111
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Increased mobilization of c-kit+ Sca-1+ Lin-(KSL)cells and colony-forming units in spleen(CFU-S)following de nove formation of a stem cell niche depends on dynamic, but not stable,membranous ossificaion (2006)
  • Author(s): Nagayoshi K, Ohkawa H, Yoro zu K, Higuchi M, Higashi S, Kubota N, Fukui H, Imai N, Gojo S, Hata J, Kobayashi Y, Umezawa A
  • Journal Title: J. Cell Physiol 208 Pages: 188-194
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] A comparison of neural differentiation and retinal transplantation with bone marrow-derived cells and retinal progenitor cells (2006)
  • Author(s): Tomita, M, Mori, T, Maruyama, K, Zahir, T, Ward, M, Umezawa, A, Young, MJ
  • Journal Title: Stem Cells 24(10) Pages: 2270-2278
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Leucine-rich repeat-containing G protein-coupled receptor-4 (LGR4, Gpr48) is essential for renal development in mice (2006)
  • Author(s): Kato, S, Matsubara, M, Matsuo, T, Mohri, Y, Kazama, I, Hatano, R, Umezawa, A, Nishimori, K
  • Journal Title: Nephron Exp Nephrol 104(2)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Differentiation of adult stem cells derived from bone marrow stoma into Leydig or adrenocortical cells (2006)
  • Author(s): Yazawa, T, Mizutani, T, Yamada, K, Kawata, H, Sekiguchi, T, Yoshino, M, Kajitani, T, Shou, Z, Umezawa, A, Miyamoto, K
  • Journal Title: Endocrinology 147(9) Pages: 4104-4111
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Increased mobilization of c-kit+ Sca-1+ Lin-(KSL)cells and colony-forming units in spleen(CFU-S)following de nove formation of a stem cell niche depends on dynamic, but not stable, membranous ossificaion (2006)
  • Author(s): Nagayoshi, K, Ohkawa, H, Yorozu, K, Higuchi, M, Higashi, S, Kubota, N, Fukui, H, Imai, N, Gojo, S, Hata, J, Kobayashi, Y, Umezawa, A
  • Journal Title: J. Cell Physiol(AU is a corresponding author) 208 Pages: 188-194
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Total cavopulmonary connection : Open anastomosis of an extracardiac conduit with vacuum-assisted venous drainage. (2006)
  • Author(s): AEBA R, Yozu R, Morita M, Matayoshi T.
  • Journal Title: Ann Thorac Surg 81 Pages: 1146-7
• [Journal Article] Use of Hemoglobin vesicle during cardiopulmonary bypass priming prevents neurocognitive decline in rat. (2006)
  • Author(s): Yamazaki M, AEBA R, Yozu R.
  • Journal Title: Circulation 114 Suppl 1
• [Journal Article] 心臓手術の実際 -外科医が語る術式、臨床工学技師が語る体外循環法- 心内膜床欠損症手術と対外循環法 -慶應義塾大学病院- (2006)
  • Author(s): 饗庭 了, 森田雅教.
  • Journal Title: クリニカルエンジニアリング 17 Pages: 1116-23
• [Journal Article] Total cavopulmonary connection : open anastomosis of an extracardiac conduit with vacuum-assisted venous drainage. (2006)
  • Author(s): Aeba R, Yozu R, Morita M, Matayoshi T.
  • Journal Title: Ann Thorac Surg. 81(3) Pages: 1146-1147
• [Journal Article] Histopathological and Behavioral Improvement of Murine Mucopolysaccharidosis Type VII by Intracerebral Transplantation of Neural Stem Cells. (2006)
  • Author(s): Fukuhara Y, Li XK, Kitazawa Y, Inagaki M, Matsuoka K, Kosuga M, Kosaki R, Shimazaki T, Endo H, Umezawa A, Okano H, Takahashi T, Okuyama T.
  • Journal Title: Mol Ther. 13(3) Pages: 548-555
• [Journal Article] Histopathological and Behavioral Improvement of Murine Mucopolysaccharidosis Type VII by Intracerebral Transplantation of Neural Stem Cells (2005)
  • Author(s): Fukuhara Y, Li XK, Kitazawa Y, Inagaki M, Matsuoka K, Kosuga M, Kosaki R, Shimazaki T, Endo H, Umezawa A, Okano H, Takahashi T, Okuyama T.
  • Journal Title: Mol Ther 13(3) Pages: 548-555
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Cell separation between mesenchymal progenitor cells through porous polymeric membranes (2005)
  • Author(s): Higuchi A, Shindo Y, Gomei Y Mori T, Uyama T, Umezawa A.
  • Journal Title: J Biomed Mater Res B Appl Biomater 74(1) Pages: 511-519
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Interleukin -6/soluble interleukin -6 receptor complex reduces infarct size via inhibiting myocardial apoptosis (2005)
  • Author(s): Matsushita K, Iwanaga S, Oda T, Kimura K, Shimada M, Sano M, Umezawa A, Hata J, Ogawa S.
  • Journal Title: Laboratory Investigation 85 Pages: 1210-1223
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Characterization and gene transfer in mesenchymal stem cells derived from human umbilical -cord blood (2005)
  • Author(s): F-Z Lu, Fujino M, Kitazawa Y Uyama T, Hara Y, Funeshima N, Jiang J-Y, Umezawa A, Li X-K
  • Journal Title: J.Lab.Clin Med 146(5) Pages: 271-278
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Membranous osteogenesis system modeled with KUSA-Al mature osteoblasts (2005)
  • Author(s): Matsumoto S, Shibuya I, Kusakari K, Segawa K, Uyama T, Shimada A, Umezawa A. AU is a corresponding author.
  • Journal Title: Biochim Biophys Acta 1725 Pages: 57-63
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Molecular and cell biological properties of mouse osteogenic mesenchymal progenitor cells, Kusa (2005)
  • Author(s): Kawashima N, Shindo K, Sakamoto K, Kondo H, Umezawa A, Kasugai S, Perbal B, Suda H, Takagi M, Katudbe K.
  • Journal Title: J.Bone Miner Metab 23 Pages: 123-133
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Laminin binding protein, 34/67 laminin receptor, carries stage-specific embryonic antigen-4 epitope defined by monoclonal antibody Raft.2 (2005)
  • Author(s): Katagiri YU, Kiyokawa N, Nakamura K, Takenouchi H, Taguchi T, Okita H, Umezawa A, Fujimoto J.
  • Journal Title: Biochem Biophys Res Commun 332 Pages: 1004-1011
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Effects of 3-methylcholanthrene on the transcriptional activity and mRNA accumulation of the oncogene hWAPL (2005)
  • Author(s): Kuroda M, Oikawa K, Yoshida K, Takeuchi A, Tekeuchi M, Usui M, Umezawa A, Mukai K.
  • Journal Title: Cancer letter 221(1) Pages: 21-28
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Combination of hTERT and Bmi-1, E6 or E7 induce prolongation of the life span of bone marrow stromal cells from an elderly donor without affecting their neurogenic potential (2005)
  • Author(s): Mori T, Kiyono T, Imabayashi 1 H, Takedal Y, Tsuchiya K, Miyoshi S, Makino H, Matsumoto H, Saito H, Ogawa S, Sakamoto M, Hata J, Umezawa A. Correspondence to AU.
  • Journal Title: Mol Cell Biol 25(12) Pages: 5183-5195
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Histopathological and Behavioral Improvement of Murine Mucopolysaccharidosis Type VII by Intracerebral Transplantation of Neural Stem Cells (2005)
  • Author(s): Fukuhara, Y, Li, XK, Kitazawa, Y, Inagaki, M, Matsuoka, K, Kosuga, M, Kosaki, R, Shimazaki, T, Endo, H, Umezawa, A, Okano. H, Takahashi. T, Okuyama. T
  • Journal Title: Mol Ther 13(3) Pages: 548-555
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Cell separation between mesenchymal progenitor cells through porous polymeric membranes (2005)
  • Author(s): Higuchi, A, Shindo, Y, Gomei, Y, Mori, T, Uyama, T, Umezawa, A
  • Journal Title: J Biomed Mater Res B Appl Biomater 74(1) Pages: 511-519
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Interleukin -6/soluble interleukin -6 receptor complex reduces infarct size via inhibiting myocardial apoptosis (2005)
  • Author(s): Matsushita, K, Iwanaga, S, Oda, T, Kimura, K, Shimada, M, Sano, M, Umezawa, A, Hata, J, Ogawa, S
  • Journal Title: Laboratory Investigation 85 Pages: 1210-1223
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Characterization and gene transfer in mesenchymal stem cells derived from human umbilical -cord blood (2005)
  • Author(s): F-Z, Lu, Fujino, M, Kitazawa, Y, Uyama, T, Hara, Y, Funeshima, N, Jiang, J-Y, Umezawa, A, Li, X-K
  • Journal Title: J. Lab. Clin Med 146(5) Pages: 271-278
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Membranous osteogenesis system modeled with KUSA-Al mature osteoblasts (2005)
  • Author(s): Matsumoto, S, Shibuya, I, Kusakari, K, Segawa, K, Uyama, T, Shimada, A, Umezawa, A
  • Journal Title: Biochim Biophys Acta(AU is a corresponding author) 1725 Pages: 57-63
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Molecular and cell biological properties of mouse osteogenic mesenchymal progenitor cells, Kusa (2005)
  • Author(s): Kawashima, N, Shindo, K, Sakamoto, K, Kondo, H, Umezawa, A, Kasugai, S, Perbal, B, Suda, H, Takagi, M, Katudbe, K
  • Journal Title: J. Bone Miner Metab 23 Pages: 123-133
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Laminin binding protein, 34/67 laminin receptor, carries stage-specific embryonic antigen-4 epitope defined by monoclonal antibody Raft. 2 (2005)
  • Author(s): Katagiri, YU, Kiyokawa, N, Nakamura, K, Takenouchi, H, Taguchi, T, Okita, H, Umezawa, A, Fujimoto, J
  • Journal Title: Biochem Biophys Res Commun 332 Pages: 1004-1011
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] K. Effects of 3-methylcholanthrene on the transcriptional activity and mRNA accumulation of the oncogene hWAPL (2005)
  • Author(s): Kuroda, M, Oikawa, K, Yoshida, K, Takeuchi, A, Tekeuchi, M, Usui, M, Umezawa, A, Mukai
  • Journal Title: Cancer letter 221(1) Pages: 21-28
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Combination of hTERT and Bmi-1, E6 or E7 induce prolongation of the life span of bone marrow stromal cells from an elderly donor without affecting their neurogenic potential (2005)
  • Author(s): Mori, T, Kiyono, T, Imabayashil, H, Takedal, Y, Tsuchiya, K, Miyoshi, S, Makino, H, Matsumoto, H, Saito, H, Ogawa, S, Sakamoto, M, Hata, J, Umezawa, A
  • Journal Title: Mol Cell Biol(Correspondence to AU) 25(12) Pages: 5183-5195
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Immortalization of human fetal cells : The life span of umbilical-cord-blood-derived cells can be prolonged without manipulating p1 6INK4a/RB braking pathway (2005)
  • Author(s): Terai, M, Uyama, T, Sugiki, T, Li, XK, Umezawa, A, Kiyono, T
  • Journal Title: Mol. Biol. Cell 16(3) Pages: 1491-1499
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Interleukin-6/soluble interleukin-6 receptor complex reduces infarct size via inhibiting myocardial apoptosis. (2005)
  • Author(s): Matsushita K, Iwanaga S, Oda T, Kimura K, Shimada M, Sano M, Umezawa A, Hata J, Ogawa S.
  • Journal Title: Lab Invest. 85(10) Pages: 1210-1223
• [Journal Article] Membranous osteogenesis system modeled with KUSA-Al mature osteoblasts. (2005)
  • Author(s): Matsumoto S, Shibuya I, Kusakari S, Segawa K, Uyama T, Shimada A. Umezawa A.
  • Journal Title: Biochim Biophys Acta. 30,1725(1) Pages: 57-63
• [Journal Article] Combination of hTERT and bmi-1, E6, or E7 induces prolongation of the life span of bone marrow stromal cells from an elderly donor without affecting their neurogenic potential. (2005)
  • Author(s): Mori T, Kiyono T, Imabayashi H, Takeda Y, Tsuchiya K, Miyoshi S, Makino H, Matsumoto K, Saito H, Ogawa S, Sakamoto M, Hata J, Umezawa A.
  • Journal Title: Mol Cell Biol. 25(12) Pages: 5183-5195
• [Journal Article] Immortalization of human fetal cells : the life span of umbilical cord blood-derived cells can be prolonged without manipulating p16INK4a/RB braking pathway. (2005)
  • Author(s): Terai M, Uyama T, Sugiki T, Li XK, Umezawa A, Kiyono T.
  • Journal Title: Mol Biol Cell. 16(3) Pages: 1491-1499
• [Journal Article] Immortalization of Human Fetal Cells : The Life Span of Umbilical Cord Blood-derived Cells Can Be Prolonged without Manipulating p16INK4a/RB Braking Pathway. (2005)
  • Author(s): Terai M, Uyama T, Sugiki T, Li XK. Umezawa A, Kiyono T.
  • Journal Title: Mol Biol Cell 16(3) Pages: 1491-1499
• [Journal Article] Immortalization of human fetal cells: The life span of umbilical-cord-blood-derived cells can be prolonged without manipulating p16INK4a/RB braking pathway (2004)
  • Author(s): Terai M, Uyama T, Sugiki T, Li XK, Umezawa A, Kiyono T
  • Journal Title: Mol.Biol.Cell 16(3) Pages: 1491-1499
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Photon-modulated changes of cell attachments on poly(spiropyran-co-methylmethacrylate) membranes (2004)
  • Author(s): Higuchi A, Hamamura A, Shindo Y, Kitamura H, Yoon B 0, Mori T, Uyama T, Umezawa A.
  • Journal Title: Biomacromolecules 5(5) Pages: 1770-1774
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Brain transplantation of genetically modified bone marrow stromal cells corrects CNS pathology and cognitive function in MPS VII mice (2004)
  • Author(s): Sakurai K, 'Iizuka S, Shen JS, Meng XL, Mori T, Umezawa A, Ohashi T, Eto Y.
  • Journal Title: Gene Therapy 11 Pages: 1475-1481
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Mesenchymal stem cells and epigenetics (2004)
  • Author(s): Umezawa A.
  • Journal Title: Brain & Development 26 Pages: 41-41
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] A custom-shaping system for bone regeneration by seeding marrow stromal cells onto a web-like biodegradable hybrid sheet (2004)
  • Author(s): Tsuchiya K, Mori T, Chen G, Ushida T, Tateishi T, Matsuno T, Sakamoto M, Umezawa A
  • Journal Title: Cell Tissue Res 316 Pages: 141-153
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Nuclear transfer of adult bone marrow mesenchymal stem cells.Developmental totipotency of tissue-specific stem cells from an adult mammal (2004)
  • Author(s): Kato Y, Imabayashi H, Mori M, Tani T, Taniguchi M, Umezawa A, Tsunoda Y.
  • Journal Title: Biol Reprod 70 Pages: 415-418
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Expression of a Novel Human Gene, Human Wings Apart-Like (hWAPL), Is Associated with Cervical Carcinogenesis and Tumor Progression (2004)
  • Author(s): Oikawa K, Ohbayashi T, Kiyono T, Nishi H, Isaka K, Umezawa A, Kuroda M, Mukai K.
  • Journal Title: Cancer Res 64 Pages: 3545-3549
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Can the life-span of human marrow stromal cells be prolonged by bmi-1, E6, E7, and/or telomerase without affecting cardiomyogenic differentiation (2004)
  • Author(s): Takeda Y, Mori T, Imabayashi H, Kiyono T, Gojo S, Miyoshi S, Ita M, Segawa K, Ogawa S, Sakamoto M, Nakamura S, Umezawa A.
  • Journal Title: J Gene Med 6(8) Pages: 833-845
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Photon-modulated changes of cell attachments on poly(spiropyran-co-methylmethacrylate) membranes (2004)
  • Author(s): Higuchi, A, Hamamura, A, Shindo, Y, Kitamura, H, Yoon, BO, Mori, T, Uyama, T, Umezawa, A
  • Journal Title: Biomacromolecules 5(5) Pages: 1770-1774
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Brain transplantation of genetically modified bone marrow stromal cells corrects CNS pathology and cognitive function in MPS VII mice (2004)
  • Author(s): Sakurai, K, Iizuka, S, Shen, JS, Meng, XL, Mori, T, Umezawa, A, Ohashi, T, Eto, Y
  • Journal Title: Gene Therapy 11 Pages: 1475-1481
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Mesenchymal stem cells and epigenetics (2004)
  • Author(s): Umezawa, A
  • Journal Title: Brain & Development 26 Pages: 41-41
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A custom-shaping system for bone regeneration by seeding marrow stromal cells onto a web-like biodegradable hybrid sheet (2004)
  • Author(s): Tsuchiya, K, Mori, T, Chen, G, Ushida, T, Tateishi, T, Matsuno, T, Sakamoto, M, Umezawa, A
  • Journal Title: Cell Tissue Res 316 Pages: 141-153
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Nuclear transfer of adult bone marrow mesenchymal stem cells. Developmental totipotency of tissue-specific stem cells from an adult mammal (2004)
  • Author(s): Kato, Y, Imabayashi, H, Mori, M, Tani, T, Taniguchi, M, Umezawa, A, Tsunoda, Y
  • Journal Title: Biol Reprod 70 Pages: 415-418
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Expression of a Novel Human Gene, Human Wings Apart-Like (hWAPL), Is Associated with Cervical Carcinogenesis and Tumor Progression (2004)
  • Author(s): Oikawa, K, Ohbayashi, T, Kiyono, T, Nishi, H, Isaka, K, Umezawa, A, Kuroda, M, Mukai, K
  • Journal Title: Cancer Res 64 Pages: 3545-3549
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Can the life-span of human marrow stromal cells be prolonged by bmi-1, E6, E7, and/or telomerase without affecting cardiomyogenic differentiation? (2004)
  • Author(s): Takeda, Y, Mori, T, Imabayashi, H, Kiyono, T, Gojo, S, Miyoshi, S, Ita, M, Segawa, K, Ogawa, S, Sakamoto, M, Nakamura, S, Umezawa, A
  • Journal Title: J Gene Med 6(8) Pages: 833-845
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Human dystrophin expression in the mdx mouse, a model of Duchenne muscular dystrophy, can be conferred predominantly by "cell fusion" with human menstrual blood-derived cells
  • Author(s): Cui C, Uyama T, Miyado K, Terai M, Kyo S, Kiyono T, Umezawa A
  • Journal Title: Mol. Biol. Cell In press
• [Journal Article] Aortic translocation with autologous tissue.
  • Author(s): AEBA R, Yozu R.
  • Journal Title: Texas Heart Institute Journal (accepted for publication)
• [Presentation] Ross-Konno Procedure for patients with Shone complex (2007)
  • Author(s): AEBA R, Yozu R.
  • Organizer: Historic inaugural meeting of The World Society for Pediatric and Congenital Heart Surgery
  • Place of Presentation: Washington DC,USA
  • Year and Date: 2007-05-04
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Ross-Konno Procedure for patients with Shone complex (2007)
  • Author(s): AEBA R, Yozu R
  • Organizer: Historic inaugural meeting o f The World Society for Pediatric and Congenital Hea rt Surgery
  • Place of Presentation: Washington DC, USA
  • Year and Date: 2007-05-04
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Ross-Konno Procedure for patients with Shone complex. (2007)
  • Author(s): AEBA R, Yozu R.
  • Organizer: Historic inaugural meeting of The World Society for Pediatric and Congenital Heart Surqery
  • Place of Presentation: Washinqton DC,USA
  • Year and Date: 2007-05-04
• [Book] 心エコーデータブック (2008)
  • Author(s): 饗庭 了、四津 良平
  • Publisher: メジカルビュー社(In press)
  • Description: 「研究成果報告書概要(和文)」より
• [Book] 心臓血管外科テクニック 弁膜症編 弁膜症の病態と治療戦略肺動脈弁狭窄症・肺動脈弁閉鎖不全症 (2008)
  • Author(s): 饗庭 了
  • Publisher: メディカ出版(In press)
  • Description: 「研究成果報告書概要(和文)」より
• [Book] 心エコーデータブック (2008)
  • Author(s): 饗庭 了.四津 良平
  • Publisher: メジカルビュー社(In Press)
• [Book] 心臓血管外科テクニック弁膜症編弁膜症の病態と治療戦略肺動脈弁狭窄症・肺動脈弁閉鎖不全症 (2008)
  • Author(s): 饗庭 了
  • Publisher: メディカ出版(In Press)
• [Book] 心臓血管外科学テキスト (2007)
  • Author(s): 饗庭 了、龍野 勝彦、重松 宏、幕内 晴朗、四津 良平、阿達 秀雄 編著
  • Publisher: 中外医学社
  • Description: 「研究成果報告書概要(和文)」より
• [Book] 心臓血管外科学テキスト (2007)
  • Author(s): 饗庭 了, 龍野 勝彦、重松 宏、幕内 晴朗、四津 良平、阿達 秀雄 編著
  • Publisher: 中外医学社