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Expression of NADEH Oxidase in human gliomas and its roles in cell proliferation and survival

Research Project

Project/Area Number 16390412
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Cerebral neurosurgery
Research InstitutionKyushu University

Principal Investigator

TADAHISA Shono (2005-2006)  Kyushu University, Hospital, Research Associate (00346793)

横山 信彦 (2004)  九州大学, 大学病院・脳神経外科, 講師 (50294939)

Co-Investigator(Kenkyū-buntansha) SASAKI Tomio  Kyushu University, Graduate School of Medical Sciences, Professor (10134561)
庄野 禎久  九州大学, 大学病院・脳神経外科, 助手 (00346793)
Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥14,300,000 (Direct Cost: ¥14,300,000)
Fiscal Year 2006: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 2005: ¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2004: ¥8,400,000 (Direct Cost: ¥8,400,000)
Keywordsapoptosis / glioma / neuroepithelial tumor / NADPH oxidase / Nox4 / proliferation / reactive oxygen species / RNAi / 免疫染色 / RT-PCR / Real time PCR / 悪性グリオーマ / RNA干渉法
Research Abstract

Reactive oxygen species (ROS) have been attracting attention as mediators of various cell signaling pathways. Nox-family NADPH oxidases have proven to be a major source of ROS production in various cell types and have crucial roles in various physiological and pathological processes. In this study, we show that Nox4, a member of Nox family, is prominently expressed in various neuroepithelial tumors by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemical studies. We quantified Nox4 mRNA expression by real-time PCR in tumor specimens from 58 patients with gliomas and found that the expression levels of Nox4 mRNA were significantly correlated with tumor grades. In addition, we show that specific knockdown of Nox4 expression by RNA interference results in cell growth inhibition and enhances induction of apoptosis by chemotherapeutic agents, such as cisplatin, in cultured glioma cell lines. Based on these observations, enhanced expression of Nox4 appears to be involved in cell proliferation and survival in glioma cells.

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (15 results)

All 2008 2007 2006 Other

All Journal Article (15 results)

  • [Journal Article] Enhanced expression of NADPH Nox4 in human gliomas and its roles in cell proliferation and survival2008

    • Author(s)
      Shono T, Yokoyama N, et. al.
    • Journal Title

      Int J Cancer (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Expression of VEGF and its receptor genes in intracranial schwannomas2007

    • Author(s)
      Uesaka T, Shono T, et. al.
    • Journal Title

      J Neurooncol 83

      Pages: 259-266

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Loss of heterozygosity analysis in an anaplastic oligodendroglioma arising after radiation therapy2007

    • Author(s)
      Hata N, Shono T, et. al.
    • Journal Title

      Neurol Res 29

      Pages: 723-726

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Enhanced expression of DNA topoisomerase II genes in human medulloblastoma and its possible association with etoposide sensitivity2007

    • Author(s)
      Uesaka T, Shono T, et. al.
    • Journal Title

      J Neurooncol 84

      Pages: 119-129

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Expression of VEGF and its receptor genes in intracranial schwannomas.2007

    • Author(s)
      Uesaka T, Shono T, et. al.
    • Journal Title

      J Neurooncol 83

      Pages: 259-266

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Loss of heterozygosity analysis in an anaplastic oligodendroglioma arising after radiation therapy.2007

    • Author(s)
      Hata N, Shono T, et. al.
    • Journal Title

      Neurol Res 29

      Pages: 723-726

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Enhanced expression of DNA topoisomerase IT genes in human medulloblastoma and its possible association with etoposide sensitivity.2007

    • Author(s)
      Uesaka T, Shono T, et. al.
    • Journal Title

      J Neurooncol 84

      Pages: 119-129

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Expression of VEGF and its receptor genes in intracranial schwannomas.2007

    • Author(s)
      Uesaka T, Shono T, et al.
    • Journal Title

      J Neurooncol (in press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Enhanced expression of DNA topoisomerase II genes in human medulloblastoma and its possible association with etoposide sensitivity.2007

    • Author(s)
      Uesaka T, Shono T, et al.
    • Journal Title

      J Neurooncol (in press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Loss of heterozygosity analysis in an anaplastic oligodendroglioma arising after radiation therapy.2007

    • Author(s)
      Hata N, Shono T, et al.
    • Journal Title

      Neurol Res (in press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Allelic loss of chromosome 10 in glioma tissues detected by quantitative signal-strand conformation polymorphism analysis2006

    • Author(s)
      Hata N, Yoshimoto K, et. al.
    • Journal Title

      Clin Chem 52

      Pages: 370-378

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Allelic loss of chromosome 10 in glioma tissues detected by quantitative signal-strand conformation polymorphism analysis.2006

    • Author(s)
      Hata N, Yoshimoto K, et. al.
    • Journal Title

      Clin Chem 52

      Pages: 370-378

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Allelic loss of chromosome 10 in glioma tissues detected by quantitative signal-strand conformation polymorphism analysis.2006

    • Author(s)
      Hata N, Yoshimoto K, et al.
    • Journal Title

      Clin Chem 52

      Pages: 370-378

    • Related Report
      2006 Annual Research Report
  • [Journal Article] An astroblastoma case associated with loss of heterozygosity on chromosome 9p.2006

    • Author(s)
      Hata N, Shono T, et al.
    • Journal Title

      J Neurooncol 80

      Pages: 69-73

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Enhanced expression of NADPH Nox4 in human gliomas and its roles in cell proliferation and survival.

    • Author(s)
      Shono T, Yokoyama N, et. al.
    • Journal Title

      Tnt 3 Cancer (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary

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Published: 2004-04-01   Modified: 2016-04-21  

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