| Project/Area Number |
16390425
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Hokkaido University |
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Principal Investigator |
YASUDA Kazunori Hokkaido University, Graduate School of Medicine, Professor, 大学院医学研究科, 教授 (20166507)
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| Co-Investigator(Kenkyū-buntansha) |
TOHYAMA Harukaz Hokkaido Univ., Hokkaido University Hospital, Assistant Professor, 北海道大学病院, 助教授 (60301884)
SHIMIZU Hiroshi Hokkaido University, Graduate School of Medicine, Professor, 大学院医学研究科, 教授 (00146672)
ONODERA Shin Hokkaido University, Graduate School of Medicine, Assistand Professor, 大学院医学研究科, 助手 (00359481)
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| Project Period (FY) |
2004 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥13,800,000 (Direct Cost: ¥13,800,000)
Fiscal Year 2006: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2005: ¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 2004: ¥6,800,000 (Direct Cost: ¥6,800,000)
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| Keywords | Soft tissues / Tendon / Ligament / Matrix / Remodeling / Fibroblast / Stress deprivation / Molecular control / 徐負荷 / 靱帯 |
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| Research Abstract |
1)Stress deprivation significantly enhanced gene expression of Mn-SOD, Tyrosinase-related protein, KIAA1199-related protein, KIAA1199, and PDGFR-alpha in fibroblasts in the patellar tendon. In addition, Mn-SOD was over-expressed in vivo at both the m-RNA and protein levels. Stress deprivation also significantly enhanced expression of Expression of IL-1beta, TNF-alpha, and TGF-beta in the patellar tendon, while it provided no significant effects to expression of PDGF-B and bFGF. In fibroblasts of the grafted tendon, VEGF was over-expressed at 2 weeks and showed a peak at 3 weeks.
2)The proliferation rate of extrinsic fibroblasts (EFs) derived from the tendon tissue was significantly lower was significantly lower than that of intrinsic fibroblasts (IFs) in the tendon. The infiltrative rate was significantly higher in EFs than in IFs on 10 to 14 days after cell culture. Ex vivo infiltration of both fibroblasts into the patellar tendon significantly reduced the mechanical properties of fasc
… More
icles from the tendon.
3)An application of TGF-beta1 significantly reduced the deterioration of mechanical properties of the patellar tendon caused by stress deprivation, while an application of anti-TGF-beta antibody significantly enhanced the degradation. Concerning the in situ frozen-thawed anterior cruciate ligament and the grafted patellar tendon, the TGF-beta1 application did not show' any significant effects. Regarding a fibrous tissue regenerated in a defect created in the patellar tendon, the TGF-beta1 application significantly increased the mechanical properties. An application of VEGF to the in situ frozen-thawed ACL significantly enhanced vascular regeneration, while it did not significantly affect the mechanical properties.
4)An application of synovial tissue-derived cells cultured in TGF-beta1-suplimented medium significantly increased the mechanical properties of the grafted tendon in ACL reconstruction and the fibrous tissue regenerated in a defect created in the patellar tendon. Less
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