• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Placental and umbilical cord blood derived mesenchymal cell for regenerative medicine and construction of a banking system for mesenchymal cell.

Research Project

Project/Area Number 16390429
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionThe University of Tokyo

Principal Investigator

TAKAHASHI Tsuneo  The University of Tokyo, Institute of Medical Science, Visiting Professor, 医科学研究所・寄付研究部門(客員教授) (50291307)

Co-Investigator(Kenkyū-buntansha) NAKAMURA Tokiko  The University of Tokyo, Institute of Medical Science, Lecturer, 医科学研究所, 講師 (90332585)
WATANABE Nobukazu  The University of Tokyo, Institute of Medical Science, Researcher Associate, 医科学研究所, 産学連携研究員 (10334278)
TOJIO Arinobu  The University of Tokyo, Institute of Medical Science, Professor, 医科学研究所, 教授 (00211681)
Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥13,800,000 (Direct Cost: ¥13,800,000)
Fiscal Year 2006: ¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 2005: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2004: ¥7,100,000 (Direct Cost: ¥7,100,000)
KeywordsPlacenta / Cord blood / Mesenchymal stem cell / Differentiation / Bone / Cartilage / Regenerative medicine / 細胞バンク / 移植 / 幹葉系細胞 / クローン
Research Abstract

We examined the possibility whether human placenta and cord blood can be a new cell source for regenerative medicine utilizing the bank system that has been established in our laboratory. First, fibroblast-like cells from fetal and maternal part were isolated by an explant method. The cells derived from fetal part were without contamination with maternal cells. Since the information required for cord blood transplantation, such as HLA typing of infant, viral and bacterial contamination in cord blood units, family history of the donor etc, were recorded in the bank, we chose chorionic villi from the fetal part as the source of mesenchymal cells in this study. The cells derived from chorionic villi (human placenta derived mesenchymal stem cells: hPDMSCs) were cultured with DMEM+10%FBS. The phenotypes of those cells were similar to those derived from bone marrow and adipose tissue; positive expression of CD29, CD44, CD73, CD105, CD90, HLA-class I, but negative for CD31, CD34, CD45,TIE-II … More and HLA-class II antigen. The hPDMSCs could differentiate to osteoblast in the osteogenic induction condition. For chondrogenic differentiation, a pellet culture system was used. The size of the pellet increased with glistening and transparency during the induction culture, and toluidine blue staining and type-II collagen immuno-histochemical staining were positive in paraffin section of the pellets. Differentiation ability to adipocyte and neural-like cell were also confirmed in the cells. The chondrogenesis of hPDMCs was also detected in vivo by transplantation of induced cells in a scaffold at subcutaneous and osteochondral defected knee of nude mice and rat.
We attempted to prolong the life span of hPDMCs by transduction with hTERT and Bmi-1 using lenti-vector, the immortalized cells kept the differentiation potential into osteogenic, chondrogenic, adipogenic cells.
Then we established the method for isolation of mesenchymal cell from cord blood (CB). The success rate of isolation of mesenchymal cell from CB increased up to 70% when the volume of CB was more than 60ml and the time between deliveries and cell isolation was shorter than 5 hours. Those cells showed high osteogenic, chondrogenic differentiation, but low for adipogenesis. The osteogenesis and chondrogensis were also observed in vivo using nude mice and rat. Thus our results indicated that human placenta and umbilical cord blood can be allogeneic mesenchymal stem cell sources for regenerative medicine. Less

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (12 results)

All 2006 2004

All Journal Article (12 results)

  • [Journal Article] Successful immortalization of mesenchymal progenitor cells derived from human placenta and the differentiation abilities of immortalized cells2006

    • Author(s)
      Zhang X, Soda Y, Takahashi K
    • Journal Title

      Biochemical and Biophysical Research Communication 351

      Pages: 853-859

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Mesenchymal progenitor cells derived from chorionic villi of human placenta for cartilage tissue engineering2006

    • Author(s)
      Zhang X, Mitsure A, Igura K
    • Journal Title

      Biochemical and Biophysical Research communication 340

      Pages: 944-952

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 臍帯血と胎盤組織由来細胞を用いた再生医療の可能性2006

    • Author(s)
      高橋 恒夫, 張暁紅, 伊倉宏一
    • Journal Title

      ティッシュエンジニアリング

      Pages: 175-186

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Successful immortalization of mesenchymal progenitor cells derived from human placenta and the differentiation abilities of immortalizes cells2006

    • Author(s)
      Xiaohong Zhang, Yasushi Soda, Kenji Takahashi, Yuansong Bai, Ayako Misturu, Koichi Igura, hitoshi Satoh, Satoru Yamaguchi, Kenzaburo Tani, Arinobu Tojo, Tsuneo A.Takahashi
    • Journal Title

      Biochemical and Biophysical Research Communication 351

      Pages: 853-859

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Mesenchymal progenitor cells derived from chorionic villi of human placenta for cartilage tissue engineering2006

    • Author(s)
      Xiaohong Zhang, Ayako Mitsuru, Koichi Igura, Kenji Takahashi, Shizuko Ichinose, Satoru Yamaguchi, Tsuneo A.Takahashi
    • Journal Title

      Biochemical and Biophysical Research Communication 340

      Pages: 944-952

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Isolation and characterization of mesenchymal progenitor cells from chorionic villi of human placenta2006

    • Author(s)
      K Igura, X Zhang, K Takahashi, A Mitsuru, S Yamaguchi, TA Takahashi
    • Journal Title

      Cytotherapy 6, No.6

      Pages: 543-553

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Successful immortalization of mesenchymal progenitor cells derived from human placenta and the differenciation abilities of immortalized cells2006

    • Author(s)
      Xiaohong Zhang, et al.
    • Journal Title

      Biochemical and Biophysical Research communication 351

      Pages: 853-859

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Mesenchymal progenitor cells derived from chorionic villi of human placenta for cartilage tissue engineering2006

    • Author(s)
      Xiaohong Zhang, et al.
    • Journal Title

      Biochemical and Biophysical Research communication 340

      Pages: 944-952

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 臍帯血と胎盤組織由来細胞を用いた再生医療の可能性2006

    • Author(s)
      高橋 恒夫
    • Journal Title

      ティッシュエンジニアリング

      Pages: 175-186

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 「研究成果報告書概要(和文)」より2004

    • Journal Title

      Vol.6 No.6

      Pages: 543-553

    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A potential pro-antiogenic cell therapy with human placenta-derived mesenchymal cells2004

    • Author(s)
      Toshihide Nishishita, kunie Ouchi, Xiaohong Zhang, Mariko Inoue, Takeshi Inazawa, Kenta Yoshiura, Koichiro Kuwabara, Takashi Nakaoka, Nobukazu Watanabe, Koichi Igura, Tsuneo A.Takahashi, Naohide Yamashita
    • Journal Title

      Biochemical and Biophysical Research Communication 325

      Pages: 24-31

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effects of osteogenic induction on mesenchymal cell from fetal and maternal parts of human placenta2004

    • Author(s)
      Kenji Takahashi, koichi Igura, Xiaohong Zhang, Ayako Mitsuru, Tsuneo A.Takahashi
    • Journal Title

      Cell Transplantation 13

      Pages: 337-341

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary

URL: 

Published: 2004-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi