Strategic Research for Upstream Molecules, Target molecules and Co-factors of the SOX trio, the Essential Transcription Factors for Chondrogenesis
| Project/Area Number |
16390430
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | The University of Tokyo |
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Principal Investigator |
IKEDA Toshiyuki The university of Tokyo, Faculty of Medicine, Visiting Research Associate, 医学部附属病院, 客員教授 (80322759)
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| Co-Investigator(Kenkyū-buntansha) |
CHUNG Ung-il The university of Tokyo, The graduate school of medicine, Associate Professor, 大学院医学系研究科, 助教授 (30345053)
KAWAGUCHI Hiroshi The university of Tokyo, Faculty of Medicine, Associate Professor, 医学部附属病院, 助教授 (40282660)
NAKAMURA Kozo The university of Tokyo, Faculty of Medicine, Professor, 医学部附属病院, 教授 (60126133)
MATSUBARA Takehiro Saitama Medical University, Advanced Tertiary Emergency Medical center, Assistant, 救命救急センター, 助手 (40361498)
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| Project Period (FY) |
2004 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥14,300,000 (Direct Cost: ¥14,300,000)
Fiscal Year 2006: ¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2005: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥7,500,000 (Direct Cost: ¥7,500,000)
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| Keywords | Chondrogenic differentiation / SOX9 / SOX6 / S100A1 / S100B / p63 / SNX19 / ノッチシグナル / II型コラーゲン / 転写因子 / レトロウィルスcDNAライブラリー / SOXトリオ / マイクロアレイ / S100 / ATDC5 |
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| Research Abstract |
We screened target molecules by the microarray analysis and identified 100A1 and B, transcriptional targets of SOX trio, inhibit terminal differentiation of chondrocytes. We established ATDC5-S2RD5 cells for real-time monitering of chondrogenic differentiation, using the SOX9 enhancer of Type II collagen and fluorescence reporter DsRed. Using ATDC5-S2RD5, we identified that BMP2 regulated Type II collagen expression mainly by MAPK signals rather than by SMAD signals. We also identified SNX19 as the novel molecule inducing chondrogenic differentiation by this system. For screening of upstream molecules of the SOX trio, we identified and characterized the CES6, the embryo enhancer of SOX6 by 5'RACE. By the south-western screening using the CES6, we identified p63 as the novel essential transcription factor of chondrogenic differentiaion, inducing SOX9, SOX6, type II collagen, etc. We also identified GNL3 as the upstream molecule of SOX6 and as co-facotr of SOX9 by this screening. Furthermore, we identified RUNX1, RUNX2, RUNX3 and GATA5 as the co-factors of SOX9 transactivating the type II collagen promoter.
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Report
(4 results)
Research Products
(16 results)
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[Journal Article] SOX2006
Author(s)
Saito, T
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Journal Title
Osteoprosis Therapy [Japanese] (5)
Pages: 70-73
Description
「研究成果報告書概要(欧文)」より
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