| Project/Area Number |
16390431
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | The University of Tokyo |
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Principal Investigator |
HOSHI Kazuto The University of Tokyo, Faculty of Medicine, Visiting Associate Professor, 医学部附属病院, 寄付講座教員(客員助教授) (30344451)
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| Co-Investigator(Kenkyū-buntansha) |
ITAKA Keishi The University of Tokyo, The graduate school of Medicine, Project Researcher, 大学院医学系研究科, 研究拠点特任教員 (60292926)
KAWAGUCHI Hiroshi The University of Tokyo, Faculty of Medicine, Associate Professor, 医学部附属病院, 助教授 (40282660)
NAKAMURA Kozo The University of Tokyo, Faculty of Medicine, Professor, 医学部附属病院, 教授 (60126133)
OGASAWARA Toru The University of Tokyo, Faculty of Medicine, Project Researcher, 医学部附属病院, 寄付講座教員 (20359623)
TEI Yuichi The University of Tokyo, Faculty of Medicine, Project Researcher, 医学部附属病院, 寄付講座教員 (30345053)
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| Project Period (FY) |
2004 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥14,400,000 (Direct Cost: ¥14,400,000)
Fiscal Year 2006: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2005: ¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2004: ¥8,300,000 (Direct Cost: ¥8,300,000)
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| Keywords | nanotechnology / tissue engineering / chondrocyte / hydrogel / soluble factor / 3D culture / proliferation / differentiation |
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| Research Abstract |
Cartilage is a major component that consists of motor and supportive tissues. Because the cartilaginous tissues suffer from various diseases and disorders including trauma, ageing diseases, inflammation and congenital anomaly, the regeneration and reconstruction of cartilage is the most important topic in the orthopaedic fields. Although the cartilage tissue engineering has gradually prevailed as autologous chondrocyte transplantation, 3D-structured tissue-engineered cartilage are needed in order to broaden the indication range of the cartilage tissue engineering for the more severe cartilage diseases such as end stage of osteoarthritis and congenital anomaly of cartilage. Otherwise, the nanotechnology has remarkably progressed, which can control the matter on a scale smaller than 1 micrometer and fabricate multipotent prolymers. In the present project, we attempted to apply the nanotechnology for the proliferation of chondrocytes in vitro and the fabrication of 3D-structured tissue-engineered cartilage.
In order to prevent dedifferentiation of cultured chondrocytes, 3D culture is useful. However, the proliferation culture of chondrocytes under 3D condition had not been reported. In the present project, we attempted to use hollow fibers for the purpose of making the 3D proliferation culture system. We also investigated the combination of soluble factors that redifferentiate dedifferentiated chondrocytes, in order to solve the problem that the chondrocytes are dedifferentiated during the proliferation culture. As results, the combination of BMP-2, insulin and T3 was revealed to induce ideal redifferentiation. Moreover, we examined the novel materials including the scaffolds which synthetic peptides possess no risk of cross-contamination and the functional micelles for gene transfection.
These results would provide useful information for the clinical application of cartilage tissue engineering.
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