| Project/Area Number |
16390443
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Tokai University |
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Principal Investigator |
MOCHIDA Joji Tokai University, SCHOOL OF MEDICINE, PROFESSOR (50174347)
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| Co-Investigator(Kenkyū-buntansha) |
SAKAI Daisuke TOKAI UNIVERSITY, SCHOOL OF MEDICINE, FELLOW (10408007)
NOMURA Takeshi TOKAI UNIVERSITY, SCHOOL OF MEDICINE, PROFESSOR (60246121)
OKUMA Masahiko TOKAI UNIVERSITY, SCHOOL OF MEDICINE, PROFESSOR (10317787)
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| Project Period (FY) |
2004 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥15,250,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2007: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2006: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥3,700,000 (Direct Cost: ¥3,700,000)
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| Keywords | cell transplantation therapy / regenerative medicine / disc regeneration / nucleus pulposus cell / mesenchymal stem cell / 骨髄間葉系幹細胞 / 共培養法 / 椎間板変性 / 臨床応用 |
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| Research Abstract |
Since only a very small number of nucleus pulposus cells could be obtained and their biological activity was low, it is necessary to develop a new activation method or find a new source of cells to replace the degenerated NP cells. Utilizing animal models, the data on the number of cells and the synthesis of DNA and proteoglycans showed that the nucleus pulposus cells were effectively activated by coculture system with bone marrow-derived mesenchymal stem cell having direct cell-to-cell contact. In vivo study using animal models such as beagle dog with disc degeneration also revealed to decelerate the velocity of progression of disc degeneration or regeneration. For the human application of the activated nucleus pulposus cells, an activation of human nucleus pulposus cells by human mesenchymal stem cells with coculture having direct cell-to-cell contact was also evaluated. The synthesis of DNA and proteoglycan revealed 5- fold higher than in the monolayer culture. No abnormality on the chromosome was revealed. Tumor genesis was not observed. Therefore, we concluded that the activated nucleus pulposus cells are very useful and safe for clinical application.
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