| Project/Area Number |
16390456
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | The University of Tokyo (2006)
Yokohama City University (2004-2005) |
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Principal Investigator |
YAMADA Yoshitsugu The University of Tokyo, Faculty of Medicine, Professor, 医学部附属病院, 教授 (30166748)
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| Co-Investigator(Kenkyū-buntansha) |
KURAHASHI Kiyoyasu Yokohama City University, Graduate School of Medicine, Associate professor, 医学部, 助教授 (50234539)
CHANG Kyungho The University of Tokyo, Faculty of Medicine, Lecturer, 医学部附属病院, 講師 (50302708)
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| Project Period (FY) |
2004 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥9,400,000 (Direct Cost: ¥9,400,000)
Fiscal Year 2006: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 2005: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2004: ¥4,200,000 (Direct Cost: ¥4,200,000)
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| Keywords | gene therapy / hyperoxia-induced injury / lung protective strategy / keratinocyte growth factor / 急性肺傷害 / アデノウイルスベクター / 緑膿菌 / 敗血症 / 高濃度酸素傷害 |
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| Research Abstract |
On the basis of recent studies dealing with acute lung injury, keratinocyte growth factor (KGF) has been shown to have certain protective effects against various injurious stimuli and to promote epithelial repair. Thus, in this study, we examined whether transient overexpression of KGF in the lung by the intratracheal administration of KGF-expressing adenovirus vector attenuates hyperoxia-induced acute lung injury in mice. The present data show that the adenovirus-mediated transfer of KGF cDNA successfully expressed high level of KGF in airway epithelial cells, and consequently raised significant lung epithelial cell proliferation, improved oxygenation, and decreased mortality. Furthermore, there was minimal vector-associated inflammation. We suggest that KGF gene transduction into lungs is a promising potential treatment for acute lung injury and would be a useful tool for regeneration research.
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