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Epigenetic profile of testicular germ cell tumors and its potential for clinical application

Research Project

Project/Area Number 16390462
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Urology
Research InstitutionShiga University of Medical Science

Principal Investigator

OKAMOTO Keisei  Shiga University of Medical Science, Department of Urology, Undergraduate School of Medicine, Assistant Professor, 医学部, 講師 (50303780)

Co-Investigator(Kenkyū-buntansha) OGAWA Osamu  Kyoto University, Department of Urology, Graduate School of Medicine, Professor, 大学院医学研究科, 教授 (90260611)
KAWAKAKAMI Takahiro  Shiga University of Medical Science, Department of Urology, Undergraduate School of Medicine, Research Assistant, 医学部, 助手 (90346023)
OKADA Yusaku  Shiga University of Medical Science, Department of Urology, Undergraduate School of Medicine, Professor, 医学部, 教授 (20127062)
杉原 洋行  滋賀医科大学, 医学部, 助教授 (30171169)
金 哲将  滋賀医科大学, 医学部, 講師 (10204968)
Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥14,100,000 (Direct Cost: ¥14,100,000)
Fiscal Year 2006: ¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 2005: ¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 2004: ¥5,900,000 (Direct Cost: ¥5,900,000)
Keywordsmethylation / testicular germ cell tumor / imprinting / DNA marker / epigenetics / 脱メチル化 / DNMT / エピジェネティックス / メチル化 / 胚細胞
Research Abstract

DNA methylation is the best known and most thoroughly studied epigenetic mechanism.
Hypermethylation of CpG islands associated with silencing of tumor suppressor genes or tumor related genes is a common hallmark of human cancer. The list of tumor related genes with aberrant hypermethylation at their CpG islands has been increasing. There is also potential for using DNA methylation profile data as markers for various types of human cancer. We show that TGCTs have distinctive DNA methylation profiles that differ from those of somatic tissue derived cancers or somatic tissues. We also discuss the methylation profile of TGCTs in terms of the DNA reprogramming that occurs in PGCs or preimplantation embryos. Finally we describe the potential clinical utility of this unique methylation phenotype in TGCTs with regard to developing a novel tumor marker. These data suggest that unmethylated DNA fragments specific in TGCTs may have diagnostic implication of this disease. Further elucidation of epigenetic profiles in TGCTs is expected to provide a new insight for biology of this disease.

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (18 results)

All 2007 2006 2005 2004

All Journal Article (18 results)

  • [Journal Article] Epigenetic profile of testicular germ cell tumours.2007

    • Author(s)
      Okamoto K, Kawakami T
    • Journal Title

      Int J Androl 30

      Pages: 385-392

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Epigenetic profile of testicular germ cell tumours2007

    • Author(s)
      Okamoto K, Kawakami T
    • Journal Title

      International Journal of Andrology (In press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Imprinted DLK1 at 14q32 is a putative tumor suppressor gene and inactivated by epimutation of a differentially methylated region upstream of GTL2 in human renal cell carcinoma.2006

    • Author(s)
      Kawakami T, Chano T, Minami K, Okabe H, Okada Y, Okamoto K
    • Journal Title

      Hum Mol Genet 15

      Pages: 821-830

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Erasure of methylation imprint at the promoter and CTCF-binding site upstream of H19 in human testicular germ cell tumors of adolescents indicate their fetal germ cell origin2006

    • Author(s)
      Kawakami T, Zhang C, Okada Y, Okamoto K
    • Journal Title

      ONCOGENE 25

      Pages: 3325-3326

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Imprinted DLK1 at 14q32 is a putative tumor suppressor gene and inactivated by epimutation of a differentially methylated region upstream of GTL2 in human renal cell carcinoma2006

    • Author(s)
      Kawakami T, Chano T, Minami K, Okabe H, Okada Y, Okamoto K
    • Journal Title

      Hum Mol Genet 15

      Pages: 821-830

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Erasure of methylation imprint at the promoter and CTCF-binding site upstream of H19 in human testicular germ cell tumors of adolescents indicate their fetal germ cell origin.2006

    • Author(s)
      Kawakami T, Zhang C, Okada Y, Okamoto K
    • Journal Title

      ONCOGENE 25

      Pages: 3225-3236

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Imprinted DLK1 at 14q32 is a putative tumor suppressor gene and inactivated by epimutation of a differentially methylated region upstream of GTL2 in human renal cell carcinoma.2006

    • Author(s)
      Kawakami T, Chano T, Minami K, Okabe H, Okada Y, Okamoto K
    • Journal Title

      Hum Mol Genet (in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Erasure of methylation imprint at the promoter and CTCF binding site upstream of H19 in human testicular germ cell tumors of adolescents indicate their fetal germ cell origin.2006

    • Author(s)
      Kawakami T, Zhang C, Okada Y, Okamoto K
    • Journal Title

      ONCOGENE (In press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Distinctive epigenetic phenotype of cancer testis antigen genes among seminomatous and non-seminomatous testicular germ cell tumors2005

    • Author(s)
      Zhang C, Kawakami T, Okada Y, Okamoto K
    • Journal Title

      Genes Chromosomes Cancer 43

      Pages: 104-112

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Distinctive epigenetic phenotype of cancer testis antigen genes among seminomatous and non-seminomatous testicular germ cell tumors.2005

    • Author(s)
      Zhang C, Kawakami T, Okada Y, Okamoto K
    • Journal Title

      Chromosomes and Cancer 43

      Pages: 104-112

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Distinctive epigenetic phenotype of cancer testis antigen genes among seminomatous and non-seminomatous testicular germ cell tumors.2005

    • Author(s)
      Okamoto K et al.
    • Journal Title

      Genes, Chromosomes and Cancer (in press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Detection of XIST unmethylated DNA fragments in male derived plasma : a potential tumor marker for testicular germ cell tumors.2004

    • Author(s)
      Kawakami T, Okamoto K, Ogawa O, Okada Y
    • Journal Title

      LANCET 363

      Pages: 40-42

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Characterization of loss-of-inactive X in Klinefelter syndrome and female derived cancer cells.2004

    • Author(s)
      Kawakami T, Zhang C, Okamoto K
    • Journal Title

      ONCOGENE 23

      Pages: 6163-6169

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Detection of XIST unmethylated DNA fragments in male derived plasma : a potential tumor marker for testicular germ cell tumors2004

    • Author(s)
      Kawakami T, Okamoto K, Ogawa O, Okada Y
    • Journal Title

      LANCET 363

      Pages: 40-42

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Characterization of loss-of-inactive X in Klinefelter syndrome and female derived cancer cells.2004

    • Author(s)
      Kawakami T, Zhang C, Okamoto K
    • Journal Title

      ONCOGENE 36

      Pages: 6163-6169

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Characterization of loss-of-inactive X in Klinefelter syndrome and female derived cancer cells.2004

    • Author(s)
      Okamoto K et al.
    • Journal Title

      ONCOGENE 23

      Pages: 6163-6169

    • Related Report
      2004 Annual Research Report
  • [Journal Article] XIST unmethylated DNA fragments in male-derived plasma as a tumor marker for testicular cancer.2004

    • Author(s)
      Okamoto K et al.
    • Journal Title

      LANCET 363

      Pages: 40-42

    • Related Report
      2004 Annual Research Report
  • [Journal Article] The MET proto-oncogene is not a major target for the gain of chromosome 7 in Testicular Germ Cell Tumors of adolescents.2004

    • Author(s)
      Okamoto K et al.
    • Journal Title

      Virchows Archive 444

      Pages: 480-481

    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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