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Analysis of the role of StAR binding protein (SBP) in the steroid hormone production

Research Project

Project/Area Number 16390470
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionHokkaido University

Principal Investigator

SUGAWARA Teruo  Hokkaido University, Grad. School of Med., Assi. Prof. (40250451)

Co-Investigator(Kenkyū-buntansha) HOSHI Nobuhiko  Kobe University, Grad. School of Agr., Professor (10209223)
MINAKAMI Hisanori  Hokkaido University, Grad. School of Med., Professor (40102256)
Project Period (FY) 2004 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥14,960,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥960,000)
Fiscal Year 2007: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2006: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2004: ¥3,900,000 (Direct Cost: ¥3,900,000)
KeywordsStAR binding protein / StAR / Cholesterol / steroid hormone / congenital lipoid adrenal hyperplasia / 卵巣 / アポトーシス / 卵巣顆粒膜細胞
Research Abstract

A role in the hormonal steroid production of the StAR protein binding protein (SBP) to combine it with the Steroidogenic acute regulatory protein (StAR) protein which adjusts hormonal steroid composition was made clear.
1. Promoter analysis of SBP It has been known that the several transcription-start sites of the SBP gene exist. There are two sites in exon 1. They are exon 1a and 1b. The reporter genes were synthesized with these promoters and transcription activation was measured. The promoter activation of 1a was compared with 1b, and it was proved that the promoter activity of 1a was 100 times higher than that of 1b in H295R cells. And, when 2μg/ml of cholesterol sulfate was added to the cultured cell, the transcription activations were increased.
2. Analysis of CS in steroidogenesis We examined the biological roles of CS in steroidogenesis in adrenocortical cells. Human adrenocortical carcinoma H295R cells were cultured with various amounts of CS. CS affects the production of steroid hormones by reducing StAR protein level in adrenocortical cells.
3. Spermatogenesis and SBP We determined whether SBP is associated with spermatogenesis, we assessed the association of SBP polymorphism in oligospermia. The frequency of the Ser allele in exon 4 was significantly lower in the oligospermia patients than in the controls. The results suggest that SBP is a susceptibility factor for spermatogenesis and that SBP is associated with male infertility.

Report

(5 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (23 results)

All 2008 2007 2006 2005 2004

All Journal Article (21 results) (of which Peer Reviewed: 7 results) Presentation (2 results)

  • [Journal Article] Activin A and eCG recover reproductive dysfunction induced by neonatal exposure to an estrogenic endocrine disruptor in adult male mice.2008

    • Author(s)
      Katsuhiko Warita, Kazutake Okamoto, Ken-ichiro Mutoh, Yoshihisa Hasegawa, Zhang-Peng Yue, Takanori Miki, Yoshiki Takeuchi, Hiroshi Kitagawa, Teruo Sugawara, Nobuhiko Hoshi.
    • Journal Title

      Biol Reprod 78

      Pages: 59-67

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cholesterol sulphate affects production of steroid hormones by reducing StAR protein level in adrenocortical cells.2007

    • Author(s)
      Teruo Sugawara, Eiji Nomura and Nobuhiko Hoshi.
    • Journal Title

      J Endocrinology 195

      Pages: 451-458

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Cholesterol sulphate affects production of steroid hormones by reducing StAR protein level in adrenocortical cells.2007

    • Author(s)
      Teruo Sugawara, Eiji Nomura and Nobuhiko Hoshi
    • Journal Title

      J Endocrinology 195

      Pages: 451-458

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Does paternal exposure to 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin(TCDD)affect the sex ratio offspring?2007

    • Author(s)
      Ishihara, K., Warita, K., Tanida, T., Sugawara, T., Kitagawa, H., Hoshi, N
    • Journal Title

      Journal of Veterinaxy Medical Science, 69

      Pages: 347-352

    • NAID

      110006250074

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cholesterol sulphate affects production of steroid hormones by reducing StAR protein level in adrenocortical cells.2007

    • Author(s)
      Teruo Sugawara, Eiji Nomura and Nobuhiko Hoshi.
    • Journal Title

      J Endocrinology; 195

      Pages: 451-458

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of a recombinant yeast assay to detect Ah-receptorligands.2006

    • Author(s)
      Teruo Sugawara, Eiji Nomura
    • Journal Title

      Toxicology Mechanisms and Methods. 16

      Pages: 287-294

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Both N-terminal and C-terminal regions of steroid sulphataseare important for enzyme activity.2006

    • Author(s)
      Teruo Sugawara, Eiji Nomura and Nobuhiko Hoshi
    • Journal Title

      J Endocrinology 188

      Pages: 365-374

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] CREM confers cAMP responsiveness in human steroidogenic acute regulatory protein expression in NCI-H295R cells rather than SF-1/Ad4BP.2006

    • Author(s)
      Teruo Sugawara, Noriaki Sakuragi and Hisanori Minakami
    • Journal Title

      J Endocrinology. 191

      Pages: 327-337

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Development of a recombinant yeast assay to detect Ah-receptor ligands.2006

    • Author(s)
      Teruo Sugawara and Eiji Nomura
    • Journal Title

      Toxicology Mechanisms and Methods 16

      Pages: 287-294

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Both N-terminal and C-terminal regions of steroid sulphatase are important for enzyme activity.2006

    • Author(s)
      Teruo Sugawara, Eiji Nomura and Nobuhiko Hoshi
    • Journal Title

      J Endocrinology 188

      Pages: 365-374

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] 「研究成果報告書概要(欧文)」より2006

    • Author(s)
      Teruo Sugawara, Noriaki Sakuragi and Hisanori Minakami
    • Journal Title

      J Endocrinology 191

      Pages: 327-337

    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Development of a recombinant yeast assay to detect Ah-receptor ligands.2006

    • Author(s)
      Teruo Sugawara, Eiji Nomura
    • Journal Title

      Toxicology Mechanisms and Methods 16・5

      Pages: 287-294

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Both N-terminal and C-terminal regions of steroid sulphatase are important for enzyme activity.2006

    • Author(s)
      Teruo Sugawara, Eiji Nomura, Nobuhiko Hoshi
    • Journal Title

      J Endocrinology 188・2

      Pages: 365-374

    • Related Report
      2006 Annual Research Report
  • [Journal Article] CREM confers cAMP responsiveness in human steroidogenic acute regulatory protein expression in NCI-H295R cells rather than SF-1/Ad4BP.2006

    • Author(s)
      Teruo Sugawara, Noriaki Sakuragi, Hisanori Minakami
    • Journal Title

      J Endocrinology 191・1

      Pages: 339-348

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Progression of the Dose-Related Effects of Estrogenic Endocrine Disruptors, an Important Factor in Declining Fertility, Differs between the Hypothalamo-Pituitary Axis an Reproductive Organs of Male Mice.2006

    • Author(s)
      Katsuhiko Warita, Teruo Sugawara, Zhan-Peng Yue, Shinji Tsukahara, Ken-ichiro Mutoh, Yoshihisa Hasegawa, Hiroshi Kitagawa, Chisato Mori, Nobuhiko Hoshi
    • Journal Title

      J of Vet Med Sci 68・2

      Pages: 125-167

    • NAID

      110005716366

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Both N-terminal and C-terminal regions of steroid sulphatase are important for enzyme activity.2006

    • Author(s)
      Teruo Sugawara
    • Journal Title

      J Endocrinology 188・2

      Pages: 365-374

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 子宮内癌におけるステロイドホルモン産生態について2005

    • Author(s)
      菅原照夫
    • Journal Title

      三井生命厚生事業団第36回「医学研究助成」研究報告集

      Pages: 14-16

    • Related Report
      2005 Annual Research Report
  • [Journal Article] The potential function of steroid sulfatase activity in steroid production and steroidogenic acute regulatory protein expression.2004

    • Author(s)
      Teruo Sugawara
    • Journal Title

      Biochemistry Journal 380

      Pages: 158-160

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Characterization of binding between SF-1 and Sp1 : SF-1 interaction with the N-terminal region of Sp1.2004

    • Author(s)
      Teruo Sugawara
    • Journal Title

      J Endocrinol Invest 27

      Pages: 133-141

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Immunohistochemical study of steroidogenic acute regulatory (StAR) protein in ovarian tumors.2004

    • Author(s)
      Teruo Sugawara
    • Journal Title

      The Internet Journal of Gynecology and Obstetrics 3

      Pages: 2-2

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Role of StAR Binding Protein in Steroidogenic Cells.2004

    • Author(s)
      Teruo Sugawara
    • Journal Title

      Endocrine Research 300

      Pages: 65-65

    • Related Report
      2004 Annual Research Report
  • [Presentation] Steroidogenic acute regulatory protein-binding protein is associated with apoptosis and controls steroidogenesis.2007

    • Author(s)
      Teruo Sugawara, Nobuhiko Hoshi, Kenji Fujieda:
    • Organizer
      ENDO 07
    • Place of Presentation
      Toronto, Canada
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] 「研究成果報告書概要(欧文)」より2007

    • Author(s)
      Teruo Sugawara, Nobuhiko Hoshi, Kenji Fujieda
    • Organizer
      ENDO07
    • Place of Presentation
      Toronto, Canada
    • Related Report
      2007 Final Research Report Summary

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Published: 2004-04-01   Modified: 2016-04-21  

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