| Project/Area Number |
16390490
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Nagoya City University |
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Principal Investigator |
UGAWA Shinya Nagoya City University, Graduate School of Medical Sciences, Associate Professor, 大学院医学研究科, 准教授 (20326135)
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| Co-Investigator(Kenkyū-buntansha) |
SHIMADA Shoichi Nagoya City University, Graduate School of Medical Sciences, Professor, 大学院医学研究科, 教授 (20216063)
FUJIMORI Osamu Nagoya City University, Graduate School of Medical Sciences, Research Worker, 大学院医学研究科, 研究員 (30128350)
MURAKAMI Shingo Nagoya City University, Graduate School of Medical Sciences, Professor, 大学院医学研究科, 教授 (80157750)
ISHIDA Yusuke Nagoya City University, Graduate School of Medical Sciences, Research Associate, 大学院医学研究科, 助教 (30381809)
KAJITA Kenji Nagoya City University, Graduate School of Medical Sciences, Research Worker, 大学院医学研究科, 研究員 (80381820)
平林 義章 名古屋市立大学, 大学院・医学研究科, 研究員 (30181184)
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| Project Period (FY) |
2004 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥12,300,000 (Direct Cost: ¥12,300,000)
Fiscal Year 2006: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 2005: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥6,700,000 (Direct Cost: ¥6,700,000)
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| Keywords | acid-sensing ion channel / amiloride / cochlea / mechano-electrical transduction / mouse / immunoelectron microscopy / in situ hybridization / ASIC1b / TRP / OTRPC4 / ASIC / Western blot |
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| Research Abstract |
We investigated whether amiloride-blockable proton-gated cation channels ASIC1 a (acid-sensing ion channel-1 a) and ASIC1 b are expressed in the stereocilia of mouse cochlear hair cells. In situ hybridization studies showed that ASIC1b transcripts, but not ASlC1 a transcripts, were expressed in the inner and outer hair cells. Fluorescent immunohistochemical and immunogold electron microscopic analyses revealed that the ASIC1b channels were located at the insertions of the stereocilia into the hair cells. Our findings provide a novel molecular key to the understanding of cochlear physiology and pathophysiology.
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