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Studies on ocular neovascularization by DNA microarray and RNA interference

Research Project

Project/Area Number 16390496
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Ophthalmology
Research InstitutionKyoto University

Principal Investigator

YOSHIMURA Nagahisa  Kyoto University, Graduate School of Medicine, Professor, 医学研究科, 教授 (70211662)

Co-Investigator(Kenkyū-buntansha) SUZUMA Kiyoshi  Kyoto University, Graduate School of Medicine, Assistant, 医学研究科, 助手 (80335265)
KATAI Naomichi  Shinshu University, School of Medicine, Lecturer, 医学部, 講師 (10260572)
TAKAGI Hitoshi  Kyoto University, Graduate School of Medicine, Lecturer, 医学研究科, 講師 (70283596)
SHIBUKI Hiroto  Shinshu University, School of Medicine, Assistant, 医学部, 助手 (70313864)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥14,000,000 (Direct Cost: ¥14,000,000)
Fiscal Year 2005: ¥6,600,000 (Direct Cost: ¥6,600,000)
Fiscal Year 2004: ¥7,400,000 (Direct Cost: ¥7,400,000)
Keywordsretinopathy of prematurity / diabetic retinopathy / retinal neovascularization / crystalline / DNA microarray / siRNA
Research Abstract

Retinal neovascularization in mice retina was produced by the method of Smith et al. The sensory retina was obtained from the model and mRNA expression changes were analyzed by Affimetrix Gene Chip MGU74 AV2. A total of 129 genes were found to be up- or down-regulated by more than twice or half of the control values in the model at P15. Among the up-regulated genes, we paid special attention to βB2-crystallin. βB2-crystallin and a-actin immunoreactivities were detected in glial cells that surrounded neovascular tufts. Short interfering RNA specific to βB2-crystallin were given to the model mice and ocular neovascularization was inhibited by the treatment. mRNA and protein expression were also inhibited by the treatment. βB2-crystallin immunoreactivities were detected in surgically obtained human tissues obtained by vitreous surgery. βB2-crystallin gene was transfected to HEK293 cells and cellular function was analyzed. In transfected cells, elongation of cellular process was observed and βB2-crystallin and a-actin were found to be co-localized underneath the cellular membrane. Our results showed that βB2-crystallin plays an important role in ocular (retinal) neovascularization.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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