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Effect of growth factor regarding lung development of congenital diaphragmatic hernia

Research Project

Project/Area Number 16390504
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Pediatric surgery
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

SUITA Sachiyo  Kyushu University, University Hospital, Director, Professor, 大学病院, 教授 (30038856)

Co-Investigator(Kenkyū-buntansha) TAGUCHI Tomoaki  Kyushu University, Graduate School of Medical Sciences, Professor, 大学院・医学研究院, 教授 (20197247)
MASUMOTO Kouji  Kyushu University, University Hospital, Assistant Professor, 大学病院, 講師 (20343329)
KINOSHITA Yoshiaki  Kyushu University, University Hospital, Research Associate, 大学病院, 助手 (80345529)
NONAKA Kazuaki  Kyushu University, Faculty of Dental Sciences, Professor, 大学院・歯学研究院, 教授 (90128067)
荻田 桂子  九州大学, 大学院・医学研究院, 助手 (40346771)
Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥14,500,000 (Direct Cost: ¥14,500,000)
Fiscal Year 2006: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2005: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2004: ¥8,700,000 (Direct Cost: ¥8,700,000)
KeywordsCongenital diaphragmatic hernia / Lung development / Insulin like growth factor / Organ culture / Fetal treatment / Gene induction / Growth hormone / Immunohistochemistry / 経胎盤的胎児麻酔 / サイトカイン / サーファクタント
Research Abstract

Insulin-like growth factors (IGFs) are one of the major growth factors related to lung development. The purpose of this study is to analyze the effect of IGF using organ cultured lungs.
The lungs were dissected from E11 to E18 mice. Total RNA was then extracted from those lungs. The expression of IGF and its receptors was analyzed by RT-PCR.
The lungs dissected from E17 mice were cultured in the BGjb medium for 48 hours. The lungs were divided into the following three groups ; the lungs were cultured only by the medium (group I), or the medium containing either IGF-1 (group II) or IGF-2 (group III). The expressions of TTF-1,Sp-C, and alpha-SMA were analyzed by both RT-PCR and immunohistochemistry.
The m-RNA expression of IGF-1 receptor was observed to be higher from E16 to E18 than at any other embryonic stages. TTF-1,Sp-C and alpha-SMA were more highly expressed in groups II and III than in those of group I. TTF-1,Sp-C, and alpha-SMA positive cells were increased in group II and III compared to group I.
Based on our findings, IGF is therefore suggested to induce alveolar maturation in the late stages of fetal lung development. IGF is considered to be one of the candidates for promoting lung development in patients with congenital diaphragmatic hernia.

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (22 results)

All 2006 2005 2004

All Journal Article (22 results)

  • [Journal Article] Increased expression of c-fos and c-jun in the rat small intestinal epithelium after ischemia-reperfusion injury : a possible correlation with the proliferation or apoptosis of intestinal epithelial cells2006

    • Author(s)
      Shima.Y, Tajiri.T, Taguchi.T, Suita.S
    • Journal Title

      Journal of Pediatric Surgery 41

      Pages: 830-836

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Experimental Study Concerning Safety Dosage of OK-432 for Intrauterine Treatment2006

    • Author(s)
      Ogita.K, Taguchi.T, Suita.S
    • Journal Title

      Asian Journal of Surgery 29(3)

      Pages: 202-206

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Relationship Between Real-Time Monitoring of the Graft Motility and Mucosal Histology in Swine Intestinal Transplantation2006

    • Author(s)
      Matsuura.T, Taguchi.T, Hayashida.M, Ogita.K, Takada.N, Nishimoto.Y, Taguchi.S, Uesugi.T, Kondo.T, Hirose.R, Suita.S
    • Journal Title

      Transplantation Proceedings 38

      Pages: 1851-1852

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Oesophageal atresia with a terminal deletion of chromosome 2q37.12006

    • Author(s)
      Masumoto.K, Suita.S, Taguchi.T
    • Journal Title

      Clinical Dysmorphology 15

      Pages: 213-216

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Safe Techniques For Inserting The Hickman Catheter In Pediatric Patients2006

    • Author(s)
      Masumoto.K, Uesugi.T, Nagata.K, Takada.N, Taguchi.S, Ogita.K, Yamanouchi.T, Taguchi.T, Suita.S
    • Journal Title

      Pediatric Hematology and Oncology 23

      Pages: 531-540

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] The Effect of a Valine-Rich Diet on Intestinal Adaptation to Massive Small Bowel Resection in the Rat2006

    • Author(s)
      Takada.N, Ogita.K, Taguchi.T, Masumoto.K, Suita.S
    • Journal Title

      Transplantation Proceedings 38

      Pages: 1830-1835

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Increased expression of c-fos and c-jun in the rat small interstinal epithelium after ischemia-reperfusion injury : a possible correlation with the proliferation or apoptosis of intestinal epithelial cells2006

    • Author(s)
      Shima.Y, Tajiri.T, Taguchi.T, Suita.S
    • Journal Title

      Journal of Pediatoric Surgery 41

      Pages: 830-836

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Relationship Between Real-Time Monitoring of the Graft Motility and Mucosal Histology in Swine Intestinal Transplantation2006

    • Author(s)
      Matsuura.T, Taguchi.T, Hayashida.M, Ogita.K, Takeda.N, Nishimoto.Y, Taguchi.S, Uesugi.T, Kondo.T, Hirose.R, Suita.S
    • Journal Title

      Transplantation Proceedings 38

      Pages: 1851-1852

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Oesophageal atresia with a terminal deletion of chromosome 2p37.12006

    • Author(s)
      Matsumoto.K, Suita.S, Taguchi.T
    • Journal Title

      Clinical Dysmorphology 15

      Pages: 213-216

    • Related Report
      2006 Annual Research Report
  • [Journal Article] An abnormal distribution of c-kit positive cells in the normoganglionic segment can predict a poor clinical outcome in patients with Hirschsprung's disease2005

    • Author(s)
      Taguchi T, Suita S, Masumoto K, Nagasaki A
    • Journal Title

      Eur J Pediatr Surg 15

      Pages: 153-158

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Efficacy of OK-432 local injection for the treatment of a neonatal branchial cleft cyst2005

    • Author(s)
      Taguchi S, Tatsuta K, Ieiri S, Taguchi T, Suita S
    • Journal Title

      Pediatr Surg Int 21

      Pages: 1004-1006

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Hirschsprung's disease in Japan : analysis of 3852 patients based on a nationwide survey in 30 years2005

    • Author(s)
      Suita S, Taguchi T, Ieiri S, Nakatsuji T
    • Journal Title

      J Pediatr Surg 40

      Pages: 197-202

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Endoscopic diverticulotomy for symptomatic pediatric esophageal diverticula2005

    • Author(s)
      Nishimoto Y, Taguchi T, Ogita K, Hashizume M, Suita S
    • Journal Title

      Pediatr Surg Int 21

      Pages: 50-53

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Effect of a valine-rich diet on a rat-model of short bowel syndrome2005

    • Author(s)
      Takada N, Ogita K, Taguchi T, Masumoto K, Suita S
    • Journal Title

      Pediatr Surg Int 21

      Pages: 899-906

    • NAID

      10028185034

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Colonic stricture secondary to hemolytic uremic syndrome caused by Escherichia coli O-1572005

    • Author(s)
      Masumoto K, Nishimoto Y, Taguchi T, Tsutsumi Y, Kanemitsu S, Hara T, Suita S
    • Journal Title

      Pediatr Nephrol 20

      Pages: 1496-1499

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Effect of liver transplantation on multiple bone fractures in an infant with end-stage biliary atresia2005

    • Author(s)
      Katsura S, Ogita K, Taguchi T, Suita S, Yoshizumi T, Soejima Y, Shimada M, Maehara Y
    • Journal Title

      Pediatr Surg Int 21

      Pages: 47-49

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Hirschsprung's disease in Japan : analysis of 3852 patients based on a nationwide survey in 30 years2005

    • Author(s)
      Suita S, Taguchi T, Ieiri S, et al.
    • Journal Title

      J Pediatr Surg 40

      Pages: 197-202

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Effect of liver transplantation on multiple bone fractures in an infant with end-stage biliary atresia : a case report2005

    • Author(s)
      Katsura S, Ogita K, Suita S, et al.
    • Journal Title

      Pediatr Surg Int 21

      Pages: 41-49

    • NAID

      10019042029

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Clinical Characteristics and the prognosis of rhabdomyosarcoma -A report from the study group for pediatric solid malignant tumors in the Kyushu area. Japan-2005

    • Author(s)
      Suita S, Noguchi S, et al.
    • Journal Title

      Eur J Pediatr Surg 15(In press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Effect of nucleoside/nucleotide-free diet in rat allogenic small intestinal transplantation2004

    • Author(s)
      Ogita K, Suita S, Taguchi T, et al.
    • Journal Title

      Pediatr Surg Int 20

      Pages: 5-8

    • NAID

      10018938362

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Efficacy of real-time monitoring to determine motility in porcine small intestinal transplantation2004

    • Author(s)
      Nishimoto Y, Taguchi T, Suita S, et al.
    • Journal Title

      Pediatr Surg Int 20

      Pages: 9-13

    • NAID

      10018938390

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Improved survival outcome for hepatoblastoma based on an optimal chemotherapeutic regimen. -A report from the study group for pediatric solid malignant tumors in the Kyushu area. Japan-2004

    • Author(s)
      Suita S, Tajiri T, et al.
    • Journal Title

      J Pediatr Surg 39

      Pages: 195-198

    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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