A new treatment approach for Malignant Melanoma basing on analysis of expression pattern of homeobox genes
| Project/Area Number |
16390505
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plastic surgery
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
YAMAMOTO Yuhei Hokkaido Univ., Grad.School of Medicine, Prof., 大学院・医学研究科, 教授 (70271674)
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| Co-Investigator(Kenkyū-buntansha) |
SASASKI Satoru Hokkaido Univ., Grad.School of Medicine, Lecturer, 大学院・医学研究科, 講師 (40301907)
FURUKAWA Hiroshi Hokkaido Univ., Hokkaido University Hospital, Pysician, 医員 (00399924)
TSUTSUMIDA Arata Hokkaido Univ., Hokkaido University Hospital, Inst., 大学院・医学研究科, 助手 (00374489)
HAMADA Jun-ichi Hokkaido Univ., Institute for Genetic Medicine, Asso.Prof., 遺伝子病制御研究所, 助教授 (50192703)
MORIUTI Tetsuya Hokkaido Univ., Institute for Genetic Medicine, Prof., 遺伝子病制御研究所, 教授 (20174394)
杉原 平樹 北海道大学, 病院・教授 (20002157)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥7,800,000 (Direct Cost: ¥7,800,000)
Fiscal Year 2005: ¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 2004: ¥3,900,000 (Direct Cost: ¥3,900,000)
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| Keywords | Malignant Melanoma / HOX gene / Metastasis / Nevus Pigmentosus |
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| Research Abstract |
HOX genes act as master genes to control morphogenesis. In human, HOX genes form 4 clusters composing 9 to 11 HOX genes (39 genes in total) on different chromosomes. We hypothesized that aberrant expression of HOX genes was associated with development and subsequent progression of melanoma and that the 39 HOX gene expression pattern determined the sites where melanoma grew. The expression levels of 39 HOX genes in 15 human cutaneous melanoma specimens and 7 nevus pigmentosus specimens were quantified by a comprehensive analysis system based on the real-time RT-PCR method. We found that the expression levels of HOXA11,A13,B9,D12 and D13 in melanoma were higher than those in nevus pigmentosus and that the expression levels of HOXA11, B2 and C13 were significantly different between pT4 melanoma and pT1 to pT3 melanoma. It was most notable that the expression levels of HOXA1,A2,C4 and B13 in melanoma with distant metastasis were higher than those in melanoma without it. On the other hand, we found no relationship between HOX genes expression patterns and the growing sites of melanoma. These results indicated that the misexpressions of some specific HOX genes were implicated in melanoma genesis and metastasis but had no linkage with melanoma sites.
And we did similar experiment of PAX genes which are also Homeobox genes.
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Report
(3 results)
Research Products
(2 results)
