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Study on tissue and vascular regeneration using adipose-derived stem cells.

Research Project

Project/Area Number 16390507
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Plastic surgery
Research InstitutionThe University of Tokyo

Principal Investigator

YOSHIMURA Kotaro  The University of Tokyo, Faculty of Medicine, Lecturer, 医学部附属病院, 講師 (60210762)

Co-Investigator(Kenkyū-buntansha) OKAZAKI Mustumi  The University of Tokyo, Faculty of Medicine, Research Associate, 医学部附属病院, 助手 (50311618)
NAGASE Takashi  The University of Tokyo, Faculty of Medicine, Research Associate, 医学部附属病院, 助手 (00359613)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥13,900,000 (Direct Cost: ¥13,900,000)
Fiscal Year 2005: ¥4,600,000 (Direct Cost: ¥4,600,000)
Fiscal Year 2004: ¥9,300,000 (Direct Cost: ¥9,300,000)
Keywordsliposuction / stem cells / adipose-derived stromal cells / SVF / CD34 / CD105 / endothelial cells / CD31 / 脂肪幹細胞 / 血管新生 / 組織増大 / 間質細胞 / 細胞外マトリックス / 細胞療法
Research Abstract

Liposuction aspirates (primarily saline solution, blood, and adipose tissue fragments) separate into fatty and fluid portions. Cells isolated from the fatty portion are termed processed lipoaspirate (PTA) cells and contain adipose-derived adherent stromal cells (ASCs). Here we define cells isolated from the fluid portion of liposuction aspirates as liposuction aspirate fluid (LAF) cells. Stromal vascular fractions (SVF) were isolated separately from both portions and characterized under cultured and non-cultured conditions. A comparable number of LAF and PLA cells were freshly isolated, but fewer LAF cells were adherent. CD34+CD45- cells from fresh LAF isolates were expanded by adherent culture, suggesting that LAF cells contain ASCs. Although freshly isolated PLA and LAF cells have distinct cell surface marker profiles, adherent PLA and LAF cells have quite similar characteristics with regard to growth kinetics, morphology, capacity for differentiation, and surface marker profiles. After plating, both PLA and LAF cells showed significant increased expression of CD29, CD44, CD49d, CD73, CD90, CD105,and CD151 and decreased expression of CD31 and CD45. Multicolor FACS analysis revealed that SVF are composed of heterogeneous cell populations including blood-derived cells (CD45+), ASCs (CD31-CD34+CD45-CD90+CD105-CD146-), endothelial (progenitor) cells (CD31+CD34+CD45-CD90+CD105lowCD146+), pericytes (CD31-CD34-CD45-CD90+CD105-CD146+), and other cells. After plating, ASCs showed a dramatic increase in CD105 expression. Although some adherent ASCs lost CD34 expression with increasing culture time, our culture method maintained CD34 expression in ASCs for at least 10-20 weeks. These results suggest that liposuction-derived cells may be useful and valuable for cell-based therapies.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (11 results)

All 2006 2005 2004 Other

All Journal Article (10 results) Book (1 results)

  • [Journal Article] Characterization of freshly isolated and cultured cells derived from the fatty and fluid portions of liposuction aspirates.2006

    • Author(s)
      kotaro Yoshimura, et al.
    • Journal Title

      journal of Cellular Physilogy (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Characterization of freshly isolated and cultured cells derived from the fatty and fluid portions of liposuction aspirates.2006

    • Author(s)
      Kotaro Yoshimura, et al.
    • Journal Title

      Journal of Cellular Physiology (In press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 幹細胞ソースとしての脂肪組織2005

    • Author(s)
      松本大輔, 吉村浩太郎
    • Journal Title

      Organ Biology 12

      Pages: 167-173

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Angle-splitting Ostectomy Followed by Facelift for Elderly Patients with Prominent Mandibular Angles.2005

    • Author(s)
      Nagase T, Yoshimura K, Aiba E, et al.
    • Journal Title

      Plastic Reconstr Surg 115

      Pages: 633-640

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Characterization of Freshly Isolated and Cultured Cells Derived from the Fatty and Fluid Portions of Liposuction Aspirates

    • Author(s)
      Kotaro Yoshimura, Tomokuni Shigeura, Daisuke Matsumoto, Takahiro Sato, Yasuyuki Takaki, Emiko Aiba, Katsujiro Sato, Keita Inoue, Takashii Nagase, Isao Koshima, Koichi Gonda
    • Journal Title

      Journal of Cellular Physiology (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Repeated treatment protocols for Melasma and Acquired Dermal Melanocytosis.

    • Author(s)
      Yoshimura K, Sato K, Aiba E, et al.
    • Journal Title

      Dermatologic Surgery (In press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Feminizing genitoplasty for treatment of XX male with masculine genitalia : case report.

    • Author(s)
      Kurita M, Aiba E, Matsumoto D, et al.
    • Journal Title

      Plastic Reconstr Surg (In press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Craniofacial Anomalies of the Cultured Mouse Embryo Induced by Inhibition of Sonic Hedgehog Signaling.

    • Author(s)
      Nagase T, Nagase M, Osumi N, et al.
    • Journal Title

      Journal of Craniofacial Surgery (In press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Clinical trial of Simultaneous Reconstruction of Breast and Well-Projected Nipple Following Expansion of Breast Skin.

    • Author(s)
      Yoshimura K, Wakita S, Sato K, et al.
    • Journal Title

      Scand J Plast Reconstr Surg Hand Surg (In press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Correlation between age and the secretions of melanocyte-stimulating cytokines in cultured keratinocytes and fibroblasts.

    • Author(s)
      Okazaki M, Yoshimura K, Uchida G, et al.
    • Journal Title

      British Journal of Dermatology (In press)

    • Related Report
      2004 Annual Research Report
  • [Book] 脂肪の再生、in再生医療工学(立石哲也、田中順三編著)2004

    • Author(s)
      吉村浩太郎
    • Publisher
      工業調査会(東京)
    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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