Study on tissue regeneration using stem cells from a fetus belonging
| Project/Area Number |
16390508
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Plastic surgery
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
HIROTAKA Asato The University of Tokyo, Faculty of Medicine, Associate Professor, 医学部附属病院, 助教授 (20222581)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
YOSHIMURA Kotaro The University of Tokyo, Faculty of Medicine, Lecturer, 医学部附属病院, 講師 (60210762)
NAGASE Takashi The University of Tokyo, Faculty of Medicine, Research Associate, 医学部附属病院, 助手 (00359613)
SARUKAWA Shunji The University of Tokyo, Faculty of Medicine, Research Associate, 医学部附属病院, 教務職員 (90324194)
|
|---|
| Project Period (FY) |
2004 – 2005
|
| Project Status |
Completed (Fiscal Year 2005)
|
| Budget Amount *help |
¥13,900,000 (Direct Cost: ¥13,900,000)
Fiscal Year 2005: ¥5,100,000 (Direct Cost: ¥5,100,000)
Fiscal Year 2004: ¥8,800,000 (Direct Cost: ¥8,800,000)
|
|---|
| Keywords | fetus belonging / umbilical cord / Wharton's jelly / mesenchymal stem cells / human umbilical cord endothelial cells / co-implantation / matrigel / neovascularization / 臍帯静脈内皮細胞 / 臍帯間質細胞 |
|---|
| Research Abstract |
We examined about establishment of collection methods of useful cells from the umbilical cord, which was disposal biomaterials, and application to the reproduction medicine. We used 0.25% trypsin /EDTA current method to umbilical cord vein and gathered Human umbilical cord vein cell(HUVEC). We used explant method to a Wharton' s jelly and gathered Human umbilical stromal cell (HUSC). In FACS analysis, our HUVEC have an approximately equal surface marker (CD31+, CD144+, Flk-1+) in comparison with commercial HUVEC. Our HUVEC also uptake acLDL, which is a basic property of an endothelium. On the other hand, HUSC was able to differentiation to cartilage, fat, bone. In FACS analysis, cells from frozen umbilical cord block have an approximately equal surface marker in comparison with cells from fresh umbilical cord block. In Matrigel assay, HUSC alone did not build network such as HUVEC, but was able to commit in a network in HUVEC. In ischemic limbs model, co-injection of HUVEC and HUSC can rescue ischemic limbs. It was thought that a neovascularization was indespensable to reproduction of the internal organs in current reproduction medicine. In the near future, using HUVEC and HUSC together well was thought to rebuild the internal organs.
|
Report
(3 results)
Research Products
(7 results)
