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Molecular mimic of group A streptococcal surface proteins

Research Project

Project/Area Number 16390525
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphological basic dentistry
Research InstitutionOsaka University

Principal Investigator

KAWABATA Shigetada  Osaka University, Graduate School of Dentistry, Associate Professor, 大学院・歯学研究科, 助教授 (50273694)

Co-Investigator(Kenkyū-buntansha) NAKAGAWA Ichiro  Tokyo University, The Institute of Medical Science, Associate Professor, 医科学研究所, 助教授 (70294113)
OKAMOTO Shigefumi  Fukuoka Dental College, Faculty of Dentistry, Assistant Professor, 歯学部, 講師 (50311759)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥14,300,000 (Direct Cost: ¥14,300,000)
Fiscal Year 2005: ¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 2004: ¥7,900,000 (Direct Cost: ¥7,900,000)
KeywordsStreptococcus pyogenes / vaccine / fibronectin / invasion / antibody / collagen / ケラチン / S.pyogenes / 分子擬態 / 免疫 / タンパク質 / 食細胞
Research Abstract

Background. Surface-associated fibronectin (Fn) binding proteins of Streptococcus pyogenes play an important role in the bacterial invasion of epithelial cells. We examined the functional domain and protective antigenicity of the Fn-binding protein FbaA. Methods. To investigate the functional domain of FbaA and its localization on S.pyogenes, a series of recombinant glutathione S-transferase (GST) truncated FbaA proteins was used for immunofluorescent microscopy, ligand blotting, and Biacore analyses. Mice were immunized with the truncated proteins for the determination of the immunogenic domains that contribute to protection against S.pyogenes infection. Results. Ligand-blotting and Biacore analyses revealed that the FbaA fragments harboring a proline-rich repeat domain (RD), but not the N- and C-terminal regions, possessed Fn-binding activity. Immunofluorescent microscopy findings showed that the N terminus and RD were exposed to external regions, which suggests that the RD serves as a Fn-binding element on live organisms. Specific antibodies were efficiently induced in N terminus and RD-immunized mice and demonstrated bactericidal activity against S. pyogenes in vitro. FbaA-immunized mice survived significantly longer than GST-immunized mice after infection with serotype M1 and M49 strains expressing FbaA. Conclusion. The Fn-binding RD and N terminus of FbaA are potential vaccine candidates for M1 strains of S.pyogenes infection.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (12 results)

All 2005 2004

All Journal Article (12 results)

  • [Journal Article] Systemic immunization with streptococcal immunoglobulin-binding protein Sib35 induces protective immunity against group A Streptococcus challenge in mice2005

    • Author(s)
      Okamoto S, Tamura Y, Terao Y, Hamada S, Kawabata S
    • Journal Title

      Vaccine 23

      Pages: 4852-4859

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Protective immunity against Streptococcus pyogenes challenge in mice following immunization with fibronectin-binding protein2005

    • Author(s)
      Terao Y, Okamoto S, Kataoka K, Hamada S, Kawabata S
    • Journal Title

      Journal of Infections Diseases 192

      Pages: 2081-2091

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 劇症型感染症をひき起こすA群レンサ球菌のゲノム解析と病原性因子2005

    • Author(s)
      浜田茂幸, 中川一路, 川端重忠
    • Journal Title

      蛋白質核酸酵素 50

      Pages: 253-261

    • NAID

      40006635196

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Systemic immunization with streptococcal immunoglobulin-binding protein Sib35 induces protective immunity against group A Streptococcus challenge in mice2005

    • Author(s)
      Okamoto S, Tamura Y, Terao Y, Hamada S, Kawabata S.
    • Journal Title

      Vaccine 23

      Pages: 4852-4859

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Protective immunity against Streptococcus pyogenes challenge in mice following immunization with fibronectin-binding protein2005

    • Author(s)
      Terao Y, Okamoto S, Kataoka K, Hamada S, Kawabata S.
    • Journal Title

      Journal of Infectious Diseases 192

      Pages: 2081-2091

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Genetic analysis and virulence factors of group A streptococci that cause severe invasive infectious diseases2005

    • Author(s)
      Hamada S, Nakagawa N, Kawabata S.
    • Journal Title

      Protein, Nucleic Acid, and Enzyme 50

      Pages: 253-261

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Systemic immunizaion with streptococcal immunoglobulin-binding protein Sib35 induces protective immunity against group A Streptococcus challenge in mice2005

    • Author(s)
      Okamoto S, Tamura Y, Terao Y, Hamada S, Kawabata S
    • Journal Title

      Vaccine 23

      Pages: 4852-4859

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Protective immunity against Streptococcus pyogenes challenge in mice following immunization with fibronectin-binding protein2005

    • Author(s)
      Terao Y, Okamoto S, Kataoka K, Hamada S, Kawabata S
    • Journal Title

      Journal of Infectious Diseases 192

      Pages: 2081-2091

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 劇症型感染症をひき起こすA群レンサ球菌のゲノム解析と病原性因子2005

    • Author(s)
      浜田 茂幸, 中川 一路, 川端 重忠
    • Journal Title

      蛋白質核酸酵素 50

      Pages: 253-261

    • NAID

      40006635196

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 劇症型感染症をひき起こすA群レンサ球菌のゲノム解析と病原性因子2005

    • Author(s)
      浜田成幸ほか
    • Journal Title

      蛋白質核酸酵素 50・3

      Pages: 253-261

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Vaccination with formalin-inactivated influenza vaccine protects mice against lethal influenza Streptococcus pyogenes superinfection2004

    • Author(s)
      Okamoto et al.
    • Journal Title

      Vaccine 22

      Pages: 2887-2893

    • Related Report
      2004 Annual Research Report
  • [Journal Article] The Streptococcus pyogenes capsule is required for adhesion of bacteria to virus-infected alveolar epithelial cells and lethal bacterial-viral superinfection2004

    • Author(s)
      Okamoto et al.
    • Journal Title

      Infection and Immunity 72・10

      Pages: 6068-6075

    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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