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Studies on Secondary Caries Initiation Mechanism and Finding Prevention Methods Using a Newly Modified Oral Biofilm Reactor

Research Project

Project/Area Number 16390544
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Conservative dentistry
Research InstitutionTokyo Medical and Dental University

Principal Investigator

MATIN Khairul  Tokyo Medical and Dental University, COE Research Associate Graduate School, Professor Junior (00372433)

Co-Investigator(Kenkyū-buntansha) TAGAMI Junji  Tokyo Medical and Dental University, Graduate School, Professor (50171567)
HANADA Nobuhiro  National Institute of Public Health, Department of Oral Health, Director (70180916)
KITASAKO Yuichi  Tokyo Medical and Dental University, Graduate School, Assistant Professor (30361702)
Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥14,000,000 (Direct Cost: ¥14,000,000)
Fiscal Year 2006: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2005: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2004: ¥11,200,000 (Direct Cost: ¥11,200,000)
KeywordsPrimary caries / Secondary caries / Cariogenic Bacteria / Oral Biofflm Reactor (QBE) / Arifieial biofilm / Self-surface-cleaning / Glucan / 2-DE / 人工口腔装置 / 人工バイオフィルム / グルカン / アルカリイオン水 / オゾン / Mass Spectrometry / biofilm / Artificial Mouth System(AMS) / mutans streptococci / fluoride oligomer / secondary caries / white spot lesion / demineralization / remineralization / Artificial Mouth System (AMS) / resin composite
Research Abstract

Newly modified computer software (EPC-2000) operated artificial mouth system (AMS) was developed and renamed as oral biofilm reactor (OBR). Automatic delivery of bacterial suspensions and mediums on to specimens became possible by controlling the flow rate in accordance of the need of a particular study simulating the human oral environments.
Biofilm induced caries models, namely; 1) Primary caries model or White Spot Enamel Lesion (WSEL) and 2) Secondary caries model were established using bovine and human extracted teeth. Visible WSELs were examined by Quantitative light-induced fluorescence (QLF), scanning electron microscope (SEM), energy dispersive X-ray spectroscope (EDS) and nanoindentor. Results indicated that demineralization occurred on the enamel surfaces. The lesion depth increased time dependently that showed co-relationship with Ca and P mineral loss and loss of nano-hardeness and elasticity. The artificial secondary caries induced by multi-species cariogenic bacteria at the interface of composite restoration showed wide gap-formation when bonding material was not used. Quantitative genomic DNA analysis (Real-time PCR) confirmed invasion of bacteria in the demineralized lesion and lesions were non-invasively indentified by Micro-CT.
Newly developed dental materials (including adhesives, fluoride oligomer incorporated coating, Teflon incorporated restoratives) were investigated for their self-surface-cleaning capability and biofilm inhibition. Also, alkali-ion water (AW) found to be effective in dissolving glucan polymers of the biofilms. Real-time PCR and 2-DE showed variations on DNA quantification and protein expression after AW or ozone treatment.

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (49 results)

All 2008 2007 2006 2005 2004 Other

All Journal Article (26 results) (of which Peer Reviewed: 6 results) Presentation (18 results) Patent(Industrial Property Rights) (5 results)

  • [Journal Article] Effect of Root Surface Coating on Biofilm Attachment Using All-in-one Adhesive Materials2008

    • Author(s)
      Daneshimehr L, Matin K, Nikaido T and Tagami J.
    • Journal Title

      Journal of Dentistry 36

      Pages: 33-41

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Surface response of fluorine-polymer incorporated resin composites to cariogenic biofilm adherence2008

    • Author(s)
      Gyo M, Nikaido T, Okada K, Yamauchi J, Tagami J.Matin K.
    • Journal Title

      Applied and Environmental Microbiology 74

      Pages: 1428-1435

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Effect of Root Surface Coating on Biofilm Attachment Using All-in-one Adhesive Materials.2008

    • Author(s)
      Daneshimehr L, Matin K, Nikaido T, Tagami J.
    • Journal Title

      J Dent 36

      Pages: 33-41

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Surface response of fluorine-polymer incorporated resin composites to cariogenic biofilm adherence.2008

    • Author(s)
      Gyo M, Nikaido T, Okada K, Yamauchi J, Tagami J, Matin K.
    • Journal Title

      Appl Environ Microbiol 74

      Pages: 1428-1435

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Surface response of fluorine-polymer incorporated resin composites to carlogenic biofilm adherence2007

    • Author(s)
      Ono M, Nikaido T, Ikeda M, Imai S, Hanada N, Tagami J and Matin K.
    • Journal Title

      Dental Materials Journal 26(5)

      Pages: 613-622

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Effect of Surface Roughness on Adherence of S.mutans Biofilms to Indirect Resin Composites2007

    • Author(s)
      Ikeda M, Matin K, Nikaido T, Foxton RM, Tagami J.
    • Journal Title

      Dental Materials Journal 26(6)

      Pages: 915-923

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Surface Properties of Resin Composite Materials Relative to Biofilm Formation.2007

    • Author(s)
      Ono M, Nikaido T, Ikeda M, Imai S, Hanada N, Tagami J, Matin K.
    • Journal Title

      Dent Mater J 26

      Pages: 613-622

    • NAID

      110006402818

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effect of Surface Roughness on Adherence of S. mutans Biofilms to Indirect Resin Composites.2007

    • Author(s)
      Ikeda M, Matin K, Nikaido T, Foxton RM, Tagami J.
    • Journal Title

      Dent Mater J 26

      Pages: 915-923

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Induction of White Spot Enamel Lesion by S.mutans Biofilm in an Artificial Mouth System and Quantification by QLF2006

    • Author(s)
      Matin K, Sultana S, Ono M, Okada A, Gyo M, Daneshmehr L.Anium A, Taeami J.
    • Journal Title

      Japan QLF Research Society Conference Proceedings Osaka Con

      Pages: 41-46

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Storage media to preserve dentin and their effects on surface properties2006

    • Author(s)
      Shamim S, Nikaido T, AsafujjohaMd, Tagami J and Matin K.
    • Journal Title

      International Chinese Journal of Dentistry 6

      Pages: 123-129

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Induction of White Spot Enamel Lesion by S. mutans Biofilm in an Artificial Mouth System and Quantification by QLF.2006

    • Author(s)
      Matin K, Sultana S, Ono M, Okada A, Gyo M, Daneshmehr L, Anjum A, Tagami J.
    • Journal Title

      Japan QLF Research Society Conference Proceedings, 2nd Osaka Conference

      Pages: 41-46

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Storage media to preserve dentin and their effects on surface properties.2006

    • Author(s)
      Shamim S, Nikaido T, Asafujjoha Md, Tagami J, Matin K.
    • Journal Title

      Int Chin J Dent 6

      Pages: 123-129

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Induction of White Spot Enamel Lesion by S. mutans Biofilm in an Artificial Mouth System and Quantification by QLF.2006

    • Author(s)
      Matin K, Sultana S, Ono M, Okada A, Gyo M, Daneshmehr L, Anjum A, Tagami J.
    • Journal Title

      Japan QLF Research Society Conference Proceedings, 2^<nd> Osaka Conference. 2^<nd> QLF Con. Edition

      Pages: 41-46

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Production of Fab fragment corresponding to surface protein antigen of Streptococcus mutans serotype c-derived peptide by Escherichia coli and Cultured tobacco cells.2006

    • Author(s)
      Yano A, Takekoshi M, Morita E, Imai S, Nisizawa T, Hanada N.
    • Journal Title

      J Biosci Bioeng. Mar;101(3)

      Pages: 251-6

    • NAID

      110004704902

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Quantitative assessment for stimulated saliva flow rate and buffering capacity in relation to different ages.2006

    • Author(s)
      Michiyo Moritsuka, Yuichi Kitasako, Michael F.Burrow, Masaomi Ikeda, Junji Tagami, Satoshi Nomura.
    • Journal Title

      Journal of Dentistry 34

      Pages: 716-720

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Storage media to preserve dentin and their effects on surface properties.2006

    • Author(s)
      Sultana S, Nikaido T, Asafujjoha Md, Tagami J, Matin K.
    • Journal Title

      Int Chin J Dent 6

      Pages: 123-129

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Induction of White Spot Enamel Lesion by S.mutans Biofilm in an artificial Mouth System and Quantification by QLF2006

    • Author(s)
      Matin K, Sultana S, Ono M, Okada A, Gyo M, Daneshmehr L, Anjum A, Tagami J.
    • Journal Title

      Japan QLF Research Society Conference Proceedings 2^<nd>(Osaka Conference)(in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Materials chemistry : A synthetic enamel for rapid tooth repair.2005

    • Author(s)
      Yamagishi K, Omuna K, Suzuki T, Okada F, Tagami J, Otsuki M, Senawangse P.
    • Journal Title

      Nature 433(7028)

      Pages: 819-819

    • Related Report
      2005 Annual Research Report
  • [Journal Article] An ingenious design for peptide vaccines.2005

    • Author(s)
      Y Akira, Onozuka A, Asahi-Ozaki Y, Imai S, Hanada N, Miwa Y, Nisizawa T.
    • Journal Title

      Vaccine 23

      Pages: 2322-2326

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Materials chemistry : A synthetic enamel for rapid tooth repair.2005

    • Author(s)
      Yamagishi K, Onuma K, Suzuki T, Okada F, Tagami J, Otsuki M
    • Journal Title

      Nature 433(7028)

      Pages: 819-819

    • Related Report
      2004 Annual Research Report
  • [Journal Article] E2fl mutation induces early onset of diabetes and Sjogren's syndrome in nonobese diabetic mice.2004

    • Author(s)
      Salam MA, Matin K, Matsumoto N, Tsuha Y. Hanada N. Senpuku H
    • Journal Title

      Journal of Immunology (J Immunol) 173(8)

      Pages: 4908-4918

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Growth-inhibitory effect of antibacterial self-etching primer on mutans streptococci obtained from arrested carious lesions.2004

    • Author(s)
      Kitasako_Y., Senpuku H., Foxton RM., Hanada N., Tagami J.
    • Journal Title

      Journal of Esthetic & Restorative Dentistry: Official Publication of the American Academy of Esthetic Dentistry. 16(3)

      Pages: 176-182

    • Related Report
      2004 Annual Research Report
  • [Journal Article] S.mutans luxS mutantによる人工バイオフィルム形成時のpH低下率2004

    • Author(s)
      Matin Khairul, 今井奨, 池田正臣, 小野雅洋, 花田信弘, 田上順次
    • Journal Title

      日本歯科医師会雑誌(0047-1763) 57巻4号

      Pages: 418-418

    • Related Report
      2004 Annual Research Report
  • [Journal Article] う蝕活動性診断を目的とした小型pHセンサーの開発2004

    • Author(s)
      北迫勇一, 村上景子, 野村聡, 中西剛, 田上順次
    • Journal Title

      日本歯科保存学雑誌(0387-2343) 47巻秋季特別

      Pages: 179-179

    • NAID

      10013752851

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Inhibition of biofilm formation using newly developed coating materials with self-cleaning properties

    • Author(s)
      Okada A, Nikaido T, Ikeda M, Okada K, Yamauchi J, Foxton RM, Sawada H, Taeami J and Matin K.
    • Journal Title

      Dental Materials Journal (印刷中)

    • NAID

      110006861867

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Inhibition of biofilm formation using newly developed coating materials with self-cleaning properties.

    • Author(s)
      Okada A, Nikaido T, Ikeda M, Okada K, Yamauchi J, Foxton RM, Sawada H, Tagami J, Matin K.
    • Journal Title

      Dent Mater J (in press)

    • NAID

      110006861867

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Evaluation of Root Surface Protection Potentials of All-in-one Adhesive Materials2007

    • Author(s)
      L Daneshmehr, K Matin, T Nikaido and J Tagami
    • Organizer
      85th IADR Meeting
    • Place of Presentation
      New Orleans,USA.
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Artificial Biofilm Induced Secondary Caries Model for in vitro Studies2007

    • Author(s)
      F Hayati, K Matin, S Malek, A Okada, Y Kitasako and J Tagami
    • Organizer
      55th Annual Meeting of JADR
    • Place of Presentation
      Tsurumi,Japan
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Two Pathways for Two Functional Waters in Controlling Cariogenic Activities2007

    • Author(s)
      Okada, K Matin, M Ono and J Tagami
    • Organizer
      55th Annual Meeting of JADR
    • Place of Presentation
      Tsurumi,Japan
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Evaluation of Root Surface Protection Potentials of All-in-one Adhesive Materials.2007

    • Author(s)
      L Daneshmehr, K Matin, T Nikaido, J Tagami.
    • Organizer
      85th IADR Meeting
    • Place of Presentation
      New Orleans, USA
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Artificial Biofilm Induced Secondary Caries Model for in vitro Studies.2007

    • Author(s)
      F Hayati, K Matin, S Malek, A Okada, Y Kitasako, J Tagami.
    • Organizer
      55th Annual Meeting of JADR
    • Place of Presentation
      Tsurumi, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Two Pathways for Two Functional Waters in Controlling Cariogenic Activities.2007

    • Author(s)
      A Okada, K Matin, M Ono, J Tagami.
    • Organizer
      55th Annual Meeting of JADR
    • Place of Presentation
      Tsurumi, Japan
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Mapping the mineral architecture in biofilm induced WSEL by SEM-EDS.2006

    • Author(s)
      K Matin, S Sultana and J Tagami
    • Organizer
      84th IADR Meeting
    • Place of Presentation
      Brisbane,Australia.
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Efficacy of OH-ion Superadded Alkali Water on Artificial Biofilm Detachment2006

    • Author(s)
      M Gyo, K Matin, A Okada, M Ono, and J Tagami
    • Organizer
      84th IADR Meeting
    • Place of Presentation
      Brisbane,Australia
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Mapping the mineral architecture in biofilm induced WSEL by SEM-EDS.2006

    • Author(s)
      K Matin, S Sultana, J Tagami.
    • Organizer
      84th IADR Meeting
    • Place of Presentation
      Brisbane, Australia
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Efficacy of Offion Superadded Alkali Water on Artificial Biofilm Detachment.2006

    • Author(s)
      M Gyo, K Matin, A Okada, M Ono, J Tagami.
    • Organizer
      84th IADR Meeting
    • Place of Presentation
      Brisbane, Australia
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Induction of White Spot Enamel Lesion by S.mutans Biofilm in an Arti facial Mouth System and Quantification by QLF.2005

    • Author(s)
      K Matin, S Sultana, M Ono, and J Tagami
    • Organizer
      2nd Japan QLF Meeting, Osaka Conference
    • Place of Presentation
      Osaka,Japan
    • Year and Date
      2005-11-03
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Induction of White Spot Enamel Lesion by S. mutans Biofilm in an Artificial Mouth System and Quantification by QLF.2005

    • Author(s)
      K Matin, S Sultana, M Ono, J Tagami.
    • Organizer
      2na Japan QLF Meeting, Osaka Conference.
    • Place of Presentation
      Osaka, Japan
    • Year and Date
      2005-11-03
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Ozone as disinfectant:effectiveness on cariogenic microbs suspended in PBS2005

    • Author(s)
      K Matin, M Ikeda and J Tagami
    • Organizer
      IADR SEA Division Meeting
    • Place of Presentation
      Malacca,Malaysia
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Biofilm induced White lesion:QLF,Nanomechanical and Fine-structural Analysis2005

    • Author(s)
      S Sultana, K Matin, M Ono and J Tagami
    • Organizer
      IADR SEA Division Meeting
    • Place of Presentation
      Malacca,Malaysia
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Ability of ozon to inactivate Streptococcus mutans in artificial mouth system2005

    • Author(s)
      M.ONO, K.MATIN, T.NIKA-DO, M.IKEDA, S.IMAI, N.HANADA, and J.TAGAMI
    • Organizer
      8th World Congress On Preventive Dentistry
    • Place of Presentation
      Liverpool,England
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Ozone as disinfectant : effectiveness on cariogenic microbs suspended in PBS.2005

    • Author(s)
      K Matin, M Ikeda, J Tagami.
    • Organizer
      IADR SEA Division Meeting
    • Place of Presentation
      Malacca, Malaysia
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Biofilm induced White lesion : QLF, Nanomechanical and Fine-structural Analysis.2005

    • Author(s)
      S Sultana, K Matin, M Ono, J Tagami.
    • Organizer
      IADR SEA Division Meeting
    • Place of Presentation
      Malacca, Malaysia
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Presentation] Ability of ozon to inactivate Streptococcus mutans in artificial mouth system.2005

    • Author(s)
      M. ONO, K. MATIN, T. NIKAIDO, M. IKEDA, S. IMAI, N. HANADA, J. TAGAMI.
    • Organizer
      8th World Congress On Preventive Dentistry
    • Place of Presentation
      Liverpool, England
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Patent(Industrial Property Rights)] 口腔洗浄剤, オゾン水製造方法, 及び口腔洗浄剤製造装置2006

    • Inventor(s)
      カイルール・マティン, ら
    • Industrial Property Rights Holder
      カイルール・マティン, ら
    • Industrial Property Number
      2006-174248
    • Filing Date
      2006-06-23
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Patent(Industrial Property Rights)] 口腔洗浄剤、オゾン水製造方法、及び口腔洗浄剤製造装置2006

    • Inventor(s)
      Khairul Matin, 〓 万里子, 岡田 彩子, 小野 雅洋, 田上 順次
    • Industrial Property Rights Holder
      東京医科歯科大学
    • Industrial Property Number
      2006-174248
    • Filing Date
      2006-06-23
    • Related Report
      2006 Annual Research Report
  • [Patent(Industrial Property Rights)] アルカリイオン水(AW)のバイォフィルム除去促進効果2005

    • Inventor(s)
      カイルール・マティン, ら
    • Industrial Property Rights Holder
      カイルール・マティン, ら
    • Industrial Property Number
      2006-174248
    • Filing Date
      2005-12-27
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Patent(Industrial Property Rights)] ホワイトスポットの形成方法(歯科研究材料)2005

    • Inventor(s)
      マティン・カイルール, シャミム・スルタナ, 暁 万里子, 小野 雅洋, 田上 順次 ら
    • Filing Date
      2005-08-31
    • Related Report
      2005 Annual Research Report
  • [Patent(Industrial Property Rights)] 歯科用重合性組成物2004

    • Inventor(s)
      沢田 英夫, 田上 順次, 二階堂 徹, マティン カイルールら
    • Filing Date
      2004-08-11
    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2021-04-07  

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