| Project/Area Number |
16390551
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
補綴理工系歯学
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| Research Institution | Niigata University |
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Principal Investigator |
UOSHIMA Katsumi Niigata University, Medical and Dental Hospital, Professor (50213400)
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| Co-Investigator(Kenkyū-buntansha) |
FUJII Noritaka Niigata University, Medical and Dental Hospital, Lecturer (90313527)
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| Project Period (FY) |
2004 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥10,070,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥570,000)
Fiscal Year 2007: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2006: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 2005: ¥4,500,000 (Direct Cost: ¥4,500,000)
Fiscal Year 2004: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | occulusal trauma / periodontal ligament / imunohistochemistry / osteopontin |
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| Research Abstract |
The purpose of this research was to establish the animal experiment model for the investigation of periodontal ligament response to the dynamic stress, and to find mechano-receptor in the periodontal ligament cells that convert a physical stress of occlusal load to a biochemical signal.
In 2004, we tried to establish an animal model. Through bonding 1 mm in diameter wire to the rat upper molar, histological changes in periodontal ligament could be investigated that was simulating occlusal trauma. As a result, the time that the periodontal ligament converted from the destruction process to the regenerative process was able to be retrieved in detail.
In addition, we selected several kinds of proteins related to the bone, and immunohistochemistry was done using the antibody to these. As a result, the production of Osteopontin was up regulated in osteoclasts. Then we tried to find RANKL production because it is thought to be working as the upstream cytokine, but we could not. This means there might be a specific pathway through which Osteopontin production is controlled.
In 2005 and 2006, we investigated histological changes of periodontal ligament under no loading condition. Because loading machine was not available, we selected the temperature stimulus instead of mechanical stimulation. As a result, the common feature of a temperature and mechanical stimulation cannot be found. Further study is required to compare these more precisely.
In 2007, we tried to establish animal model that enables us to investigate thermal influences to the periodontal ligament, but we could not achieve because we could not fabricate a handy device for this purpose. Further study is necessary.
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