| Project/Area Number |
16390589
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Osaka University |
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Principal Investigator |
KATO Itsuro Osaka Univ., Graduate School of Dentistry, Assistant Professor, 大学院・歯学研究科, 助手 (60314390)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAZAWA Mitsuhiro Osaka Univ., Hospital Fac.of Dent., Assistant Professor, 歯学部附属病院, 講師 (70217701)
YURA Yoshiaki Osaka Univ., Graduate School of Dentistry, Professor, 大学院・歯学研究科, 教授 (00136277)
SAKURAI Yoshinori Kyoto Univ., Research Reactor Institute, Assistant Prof., 原子炉実験所, 助手 (20273534)
KOBAYASHI Toru Kyoto Univ., Research Reactor Institute, Associate Prof., 原子炉実験所, 助教授 (90089136)
ONO Koji Kyoto Univ., Research Reactor Institute, Professor, 原子炉実験所, 教授 (90122407)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥12,700,000 (Direct Cost: ¥12,700,000)
Fiscal Year 2005: ¥5,300,000 (Direct Cost: ¥5,300,000)
Fiscal Year 2004: ¥7,400,000 (Direct Cost: ¥7,400,000)
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| Keywords | human squamous cell carcinoma / BNCT / BPA / BSH |
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| Research Abstract |
We recently reported that boron neutron capture therapy (BNCT) represents a new and promising treatment approach even for huge or far advanced head and neck malignancies. But the mechanism of cell death from BNCT has not clarified yet. Especially focused on p53 status, we investigated effects of BNCT on cell growth, apoptosis and cell cycle related p53. We used SAS/neo cell with wild type p53 and SAS/mp53 cell with mutant type p53, both are identical genetic backgrounds except for the p53 expression.
The cancer cells were irradiated with neutron beam at Kyoto University Research Reactor after incubation with p-boronopheylalanine for 2 hours. Surviving fraction of each cell was measured by colonogenic assay.
Cell growth was measured by MTT assay. Cell cycles were analyzed by flow cytometric assay. BNCT-induced apoptotic bodies were detected by Hoechst33342 stain.
Results are as follows. (1) Colony formation assay revealed the number of SAS/neo cell-colony formation were suppressed, physical dose-dependently. (2) When SAS/neo cell were irradiated at 2Gy and 6Gy (physical dose), MTT assay showed the growth suppression rate of the cell, compared with that of SAS/mp53 cell were 80% and about 70% respectively for 48 hours. (3) Flow cytometric assay and Hoechst stain revealed that apoptosis rates were increased in SAS/neo cell compared with that in SAS/mp53 time-dependently.
These data suggests that SAS/mp53 cell was more resistant to BNCT than SAS/neo cell physical dose-dependently. Major tumor suppression gene, p53 status of head and neck cancer might influence on the outcomes of BNCT.
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