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The association of human papillomavirus with carcinogenesis of oral squamous cell carcinoma.

Research Project

Project/Area Number 16390590
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionHiroshima University

Principal Investigator

SUGIYAMA Masaru  Hiroshima University, Faculty of Dentistry, Professor (70187681)

Co-Investigator(Kenkyū-buntansha) MIYAUCHI Miwa  Hiroshima University, Hospital, Lecturer (10325201)
HIGASHIKAWA Koichiro  Hiroshima University, Graduate School of Biomedical Sciences, Assistant Professor (80363084)
Project Period (FY) 2004 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥14,500,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥900,000)
Fiscal Year 2007: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2006: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2005: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2004: ¥4,400,000 (Direct Cost: ¥4,400,000)
Keywordssquamous cell carcinoma / human papillomavirus / carcinogenesis / 細胞周期調節因子 / 5年生存率 / 統計解析
Research Abstract

This study was performed to clarify the association of HPV with oral squamous cell carcinoma and the following results were obtained.
(1) We compared the PGMY09/PGMY11 L1 primers amplifying HPV L1 region and the E6 primers in Takara PCR Human Papillomavirus Detection Kit. Both primers showed similar sensitivity, but the latter showed better specificity. (2) DNAs obtained from fresh frozen tissues, formalin-fixed paraffin embedded sections, or swab samples were amplified by using the Takara primers. Similar sensitivity was observed in both fresh frozen tissues and swab samples, but sensitivity was low in paraffin embedded sections. (3) We analyzed DNAs eluted from swab samples obtained from tumor, tumor margin and normal part. More HPV DNA copies were observed in tumor part compared with the other parts. (4) HPV-18 DNA copy number was high in the patient with multiple recurrences, but not in the other patients and normal controls. (5) Neither HPV-16 nor HPV-18 DNA was observed in DNAs obtained from formalin-fixed paraffin embedded sections of metastasized lymph nodes. (6) Patients with HPV E7 DNA showed better prognosis compared with the counterparts. (7) Expression of Cdc25A, cell cycle regulator, was positively related to expression of HPV E7 and another cell cycle regulator, 14-3-3, was negatively related to expression of HPV E6.

Report

(5 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (12 results)

All 2007 2005 Other

All Journal Article (9 results) (of which Peer Reviewed: 5 results) Presentation (3 results)

  • [Journal Article] Human papillomavirus-16 in oral squamous cell carcinoma: Clinical correlates and 5-year survival.2007

    • Author(s)
      Masaru Sugiyama
    • Journal Title

      British Journal of Oral and Maxillofacial Surgery 45

      Pages: 116-122

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] High-risk human papillomavirus type 16 E7 oncogene associates with Cdc25A over-expression in oral squamous cell carcinoma.2007

    • Author(s)
      Ujjal Kumar Bhawal
    • Journal Title

      Virchows Arch 450

      Pages: 65-71

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Human papillomavirus-16 in oral squamous cell carcinoma : Clinical correlates and 5-year survival2007

    • Author(s)
      Masaru, Sugiyama
    • Journal Title

      British J Oral Maxillofac Surg 45

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] High-risk human papillomavirus type 16 E7 oncogene associates with Cdc25A over-expression in oral squamous cell carcinoma2007

    • Author(s)
      Ujjal, Kumar, Bhawal
    • Journal Title

      Virchows Arch 450

      Pages: 65-71

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Human papillomavirus-16 in oral squamous cell carcinoma: Clinical correlates and 5-year survival2007

    • Author(s)
      Masaru Sugiyama
    • Journal Title

      British Journal of Oral and Maxillofacial Surgery 45

      Pages: 116-122

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] High-risk human papillomavirus type 16 E7 oncogene associates with Cdc25A over-expression in oral squamous cell carcinoma2007

    • Author(s)
      Ujjal Kumar Bhawal
    • Journal Title

      Virchows Arch 450

      Pages: 65-71

    • Related Report
      2007 Annual Research Report 2006 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Human papillomavirus-16 in oral squamous cell carcinoma; Clinical correlates 5-year survial2007

    • Author(s)
      Masaru Sugiyama
    • Journal Title

      British Journal of Oral and Maxillofacial Surgery 45

      Pages: 116-122

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Loss of 14-3-3 sigma and human papillomavirus 16E6 presence in oral squamous cell carcinoma.

    • Author(s)
      Ujjal Kumar Bhawal
    • Journal Title

      Archives of Otolaryngology-Head & Neck Surgery (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Loss of 14-3-3 sigma and human papillomavirus 16E6 presence in oral squamous cell carcinoma

    • Author(s)
      Ujjal, Kumar, Bhawal
    • Journal Title

      Arch Otolaryngol-Head & Neck Surg (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] The association of Cdc25A over-expression with human papillomavirus type 16 in oral squamous cell carcinoma2007

    • Author(s)
      Masaru, Sugiyama
    • Organizer
      The 62nd Annual Meeting of the Japanese Stomatological Society
    • Place of Presentation
      Fukuoka
    • Year and Date
      2007-04-18
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 口腔扁平上皮癌におけるヒトパピローマウイルス16型の存在と5年生存率との相関について.2005

    • Author(s)
      杉山 勝
    • Organizer
      第38回広島大学歯学会
    • Place of Presentation
      広島
    • Year and Date
      2005-06-18
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] The association of human papillomavirus-16 in oral squamous cell carcinoma with 5-year survival2005

    • Author(s)
      Masaru, Sugiyama
    • Organizer
      The 38th Conference of Hiroshima University Dental Society
    • Place of Presentation
      Hiroshima
    • Year and Date
      2005-06-18
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2004-04-01   Modified: 2016-04-21  

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