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Mechanisms of multipotency of adult stem cells derived from bone marrow stroma and peripheral blood and their application for regenerative medicine

Research Project

Project/Area Number 16390591
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionThe University of Tokushima

Principal Investigator

NAGAYAMA Masaru  The University of Tokushima, Institute of Health Biosciences, Professor, 大学院ヘルスバイオサイエンス研究部, 教授 (30022867)

Co-Investigator(Kenkyū-buntansha) SATOMURA Kazuhito  The University of Tokushima, Institute of Health Biosciences, Associate Professor, 大学院ヘルスバイオサイエンス研究部, 助教授 (80243715)
FUJISAWA Kenji  The University of Tokushima, University Hospital, Lecturer, 医学部・歯学部附属病院, 講師 (40228979)
NAKANISHI Hiroaki  The University of Tokushima, University Hospital, Lecturer, 医学部・歯学部附属病院, 講師 (00243717)
TAKECHI Masaaki  The University of Tokushima, Institute of Health Biosciences, Assistant Professor, 大学院ヘルスバイオサイエンス研究部, 助手 (00304535)
北岡 栄一郎  徳島大学, 医学部・歯学部附属病院, 助手 (60343307)
Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥14,500,000 (Direct Cost: ¥14,500,000)
Fiscal Year 2006: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2005: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2004: ¥10,200,000 (Direct Cost: ¥10,200,000)
KeywordsRegenerative medicine / Tissue regenaration / Stem cell / Bone marrow stromal cell / Induced differentiation / 多分化能 / 体性幹細胞
Research Abstract

For realization of ideal reproductive medicine, it is extremely important to secure the stable supply of stem cells, and reproduction of organs and tissues using patients' own somatic cells is expected to realize ideal therapy without a number of restrictions in cases of using ES cells. From this point of view, we here focused on multipotency of bone marrow stromal cells and investigated the potential application for reproductive medicine.
First, several lines of bone marrow stromal cells were established from mouse bone marrow. We successfully established a new method to induce differentiation of these cells into mesodermal cells such as osteoblasts, cartilage cells, muscle cells, and adipocytes, ectodermal cells such as nerve cells, and endodermal cells such as hepatocytes by culture with various growth factors, vitamins, and extracellular matrix. Intriguingly, it was possible to induce nerve cells and hepatocytes at almost 100% under these induction conditions. These results indicate … More d that multipotent adult stem cells were present in bone marrow stromal cells.
Next, the green fluorescent protein (GFP) gene was introduced to these bone marrow stromal cell lines exhibiting multipotency in vitro and stable cell lines expressing GFP were established. During the GFP gene introduction, some cell lines showed different degrees of differentiation. After additional in vitro examination, multipotent culture cells were inoculated in the subcutaneous tissue, muscles, brain, liver, artificially created areas lacking bones and cartilages, and spinal injury sites in immunodeficient mice, and tissues and organs containing the inoculation site were collected over time to histologically examine the localization and distribution of transplanted cells.
As a result, some mouse bone marrow stromal cell lines newly established in this study showed complete multipotency while others revealed limited multipotency. Therefore, exploration of genes involved in maintenance of an undifferentiated state is underway by comparing gene expression in these different phenotypes. Less

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (15 results)

All 2007 2006 2005 2004 Other

All Journal Article (14 results) Book (1 results)

  • [Journal Article] Melatonin at pharmacological doses enhances human osteoblastic differentiation in vitro and promotes mouse cortical bone formation in vivo.2007

    • Author(s)
      Kazuhito Satomura
    • Journal Title

      Journal of Pineal Research 42・2

      Pages: 231-239

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Expression of melatonin 1a receptor in the tooth germ.2007

    • Author(s)
      Kazuhito Satomura
    • Journal Title

      Journal of Dental Research 36・Special Isuue A

      Pages: 606-606

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Specific Wavelength Visible Light Irradiation Inhibits Bacterial Growth of Porphyromonas gingivalis2007

    • Author(s)
      Makoto Fukui
    • Journal Title

      Journal of Periodontal Research (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Melatonin at pharmacological doses enhances human osteoblastic differentiation in vitro and promotes mouse cortical bone formation in vivo2007

    • Author(s)
      Kazuhito Satomura
    • Journal Title

      Journal of Pineal Research 42-2

      Pages: 231-239

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Expression of melatonin la receptor in the tooth germ2007

    • Author(s)
      Kazuhito Satomura
    • Journal Title

      Journal of Dental Research 36-Special issue A

      Pages: 606-606

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Melatonin at pharmacological doses enhances human osteoblastic differentiation in vitro and promotes mouse cortical bone formation in vivo.2007

    • Author(s)
      Kazuhito Satomura
    • Journal Title

      Journal of Pineal Research 41・2

      Pages: 231-239

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Expression of melatonin la receptor in the tooth germ.2007

    • Author(s)
      Kazuhito Satomura
    • Journal Title

      Journal of Dental Research 36・Special Issue A

      Pages: 606-606

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Proliferation and differentiation of cultured MC3T3-El osteoblasts on surface-layer modified hydroxyapatite ceramic with acid and heat treatments.2005

    • Author(s)
      Tetsuya Yuasa
    • Journal Title

      Dental Materials Journal 24・2

      Pages: 207-212

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Proliferation and differentiation of cultured MC3T3-E1 osteoblasts on surface-layer modified hydroxyapatite ceramic with acid and heat treatments2005

    • Author(s)
      Tetsuya Yuasa
    • Journal Title

      Dental Materials Journal 24-2

      Pages: 207-212

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] ラット骨形成におけるマスピンの役割2004

    • Author(s)
      徳山 麗子
    • Journal Title

      四国歯学会雑誌 17・1

      Pages: 159-175

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effects of apatite cements on proliferation and differentiation of human osteoblasts in vitro.2004

    • Author(s)
      Tetsuya Yuasa
    • Journal Title

      Biomaterials 25

      Pages: 1159-1166

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Potential role of maspin in rat endochondral ossification2004

    • Author(s)
      Reiko Tokuyama
    • Journal Title

      Shikoku Dental Research 17-1

      Pages: 159-175

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effects of apatite cements on proliferation and differentiation of human osteoblasts in vitro2004

    • Author(s)
      Tetsuya Yuasa
    • Journal Title

      Biomaterials 25

      Pages: 1159-1166

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Specific wavelength visible light irradiation inhibits bacterial growth of Porphyromonas gingivalis

    • Author(s)
      Makoto Fukui
    • Journal Title

      Journal of Periodontal Research (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Book] 歯の再生 -歯の発生生物学から歯の再生研究まで-2006

    • Author(s)
      里村 一人
    • Total Pages
      271
    • Publisher
      真興交易(株)医書出版部
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary

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Published: 2004-04-01   Modified: 2016-04-21  

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