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Profiling of oral bacterial flora using DNA chips and its application to the risk evaluation of oral diseases of children.

Research Project

Project/Area Number 16390601
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthodontic/Pediatric dentistry
Research InstitutionTohoku University

Principal Investigator

TAKAHASHI Nobuhiro  Tohoku University, Grad Sch of Dentistry, Professor, 大学院歯学研究科, 教授 (60183852)

Co-Investigator(Kenkyū-buntansha) HATA Shinji  Tohoku University, Hospital, Lecturer, 病院・講師 (20208507)
SATO Takuichi  Tohoku University, Grad Sch of Dentistry, Lecturer, 大学院歯学研究科, 講師 (10303132)
MATSUYAMA Junko  Niigata University, Grad Sch of Medical Dental Sciences, Assistant, 医歯学系, 助手 (30293236)
NAKAJO Kazuko  Tohoku University, Grad Sch of Dentistry, Assistant, 大学院歯学研究科, 助手 (40374946)
WASHIO Jumpei  Tohoku University, Grad Sch of Dentistry, Assistant, 大学院歯学研究科, 助手 (20400260)
真柳 秀昭  東北大学, 大学院・歯学研究科, 教授 (60005098)
野田 忠  新潟大学, 医歯学系, 教授 (00013970)
Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥12,400,000 (Direct Cost: ¥12,400,000)
Fiscal Year 2006: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2005: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2004: ¥6,400,000 (Direct Cost: ¥6,400,000)
KeywordsDNA chips / 16S ribosomal RNA / DNA probe / DNA primers / Oral bacteria
Research Abstract

Oral biofilms have been reported to be associated with oral infections such as dental caries and periodontitis. And also, oral biofilms are microbial ecosystems which contain various amounts and sorts of microorganisms, and thus controlling these microbial florae are thought to be one of the ways toward prevention of these oral infections. The aim of this study was to establish DNA micro arrays system to detect oral infections-associated bacteria utilizing species-specific DNA probes of 16S ribosomal RNA genes. Twenty dentinal endodontic lesions-associated bacteria including mutans streptococci and twenty-three periodontitis-associated bacteria including Porphyromonas gingivalis, Tannerella forsythia and Mogibacterium timidum were selected in this study, and the specificity and sensitivity of the DNA micro arrays were tested. In addition, we have been planning to compare the DNA micro arrays system with the direct-& real-time PCR on their specificities and sensitivities utilizing one-hundred subgingival plaque biofilms for the present.

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (19 results)

All 2007 2006 2005 2004

All Journal Article (18 results) Book (1 results)

  • [Journal Article] Profiling of bacterial flora in crevices around titanium orthodontic anchor plates2007

    • Author(s)
      Sato R
    • Journal Title

      Clin Oral Implant Res 18(1)

      Pages: 21-26

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Resistance to acidic and alkaline environments in the endodontic pathogen Enterococcus faecalis2006

    • Author(s)
      Nakajo K
    • Journal Title

      Oral Microbiol Immunol 21(5)

      Pages: 283-288

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Difference in the xylitol sensitivity of acid production among Streptococcus mutans strains, and its biochemical mechanism2006

    • Author(s)
      Miyasawa-Hori H
    • Journal Title

      Oral Microbiol Immunol 21(4)

      Pages: 201-205

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Resistance to acidic and alkaline environments in the endodontic pathogen Enterococcus faecalis.2006

    • Author(s)
      Nakajo K
    • Journal Title

      Oral Microbiol Immunol 21(5)

      Pages: 283-288

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Difference in the xylitol sensitivity of acid production among Streptococcus mutans strains, and its biochemical mechanism.2006

    • Author(s)
      Miyasawa-Hori H
    • Journal Title

      Oral Microbiol Immunol 21(4)

      Pages: 201-205

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Comparison of age-dependent expression of aggrecan and ADAMTSs in mandibular condylar cartilage, tibial growth plate, and azticular cartilage in rats2006

    • Author(s)
      Mitani H
    • Journal Title

      Histochem Cell Biol 126(3)

      Pages: 371-380

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 培養ヒト歯根膜細胞由来上皮細胞と線維芽細胞の境界面におけるオステオポンチンおよびオステオカルシンの発現2006

    • Author(s)
      佐藤充太
    • Journal Title

      日歯保存誌 49(1)

      Pages: 92-98

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Difference in the xylitol sensitivity of acid production among Streptococcus mutans strains, and its biochemical mechanisim.2006

    • Author(s)
      Miyasawa-Hori H
    • Journal Title

      Oral Microbiol Immunol 21(in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Resistance to acidic and alkaline environments in the endodontic pathogen Enterococcus faecalis.2006

    • Author(s)
      Nakajo K
    • Journal Title

      Oral Microbiol Immunol 21(in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Hydrogen sulfide-producing bacteria in tongue coating and their relationship with oral malodour.2005

    • Author(s)
      Washio J
    • Journal Title

      J Med Microbiol 54(9)

      Pages: 889-895

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] Quantification and detection of bacteria from postoperative maxillary cyst by polymerase chain reaction.2005

    • Author(s)
      Yamaura M
    • Journal Title

      Oral Microbiol Immunol 20(6)

      Pages: 333-338

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2005 Annual Research Report
  • [Journal Article] Synergistic inhibition by combination of fluoride and xylitol on the glycolysis by mutans streptococci and its biochemical mechanism.2005

    • Author(s)
      Maehara H
    • Journal Title

      Caries Res 39(6)

      Pages: 521-528

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Expression of type IV collagen and laminin at the interface between epithelial cells and fibroblasts from human periodontal ligament.2005

    • Author(s)
      Shimonishi M
    • Journal Title

      Eur J Oral Sci 113(1)

      Pages: 34-40

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Detection frequency of periodontitis-associated bacteria by polymerase chain reaction in subgingival and supragingival plaque of periodontitis and healthy subjects.2004

    • Author(s)
      Mayanagi G
    • Journal Title

      Oral Microbiol Immunol 19(6)

      Pages: 379-385

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] A method for mapping the distribution pattern of cariogenic streptococci within dental plaque in vivo.2004

    • Author(s)
      Kato K
    • Journal Title

      Caries Res 38(5)

      Pages: 448-453

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] PTC gene mutation and expression of SHH, PTC, SMO, and GLI-1 in odontogenic keratocysts.2004

    • Author(s)
      Ohki K
    • Journal Title

      Int J Oral Maxillofac Surg 33(6)

      Pages: 584-592

    • Related Report
      2004 Annual Research Report
  • [Journal Article] p53 gene status and expression of p53, MDM2, and p14ARF proteins in ameloblastomas.2004

    • Author(s)
      Kumamoto H
    • Journal Title

      J Oral Pathol Med 33(5)

      Pages: 292-299

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Alkali resistant bacteria in root canal systems.2004

    • Author(s)
      Nakajo K
    • Journal Title

      Oral Microbiol Immunol 19(6)

      Pages: 390-394

    • Related Report
      2004 Annual Research Report
  • [Book] 口腔生化学 第4版2005

    • Author(s)
      早川太郎, 須田立雄, 木崎治俊, 畑隆一郎, 高橋信博, 宇田川信之
    • Publisher
      医歯薬出版株式会社
    • Related Report
      2005 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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