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Visualization of osteocyte differentiation in living bone

Research Project

Project/Area Number 16390606
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthodontic/Pediatric dentistry
Research InstitutionOkayama University

Principal Investigator

KAMIOKA Hiroshi  Okayama University, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Associate Professor, 大学院・医歯薬学総合研究科, 助教授 (80253219)

Co-Investigator(Kenkyū-buntansha) TAKANO Teruko (YAMAMOTO Teruko)  Tohoku University, Graduate School of Dentistry, Professor, 大学院・歯学研究科, 教授 (00127250)
FUKUNAGA Tomohiro  Okayama University, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Research Associate, 大学院・医歯薬学総合研究科, 助手 (70362994)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥14,600,000 (Direct Cost: ¥14,600,000)
Fiscal Year 2005: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2004: ¥13,100,000 (Direct Cost: ¥13,100,000)
Keywordsosteocytes / osteoblasts / 3D reconstruction / bone / actin filament
Research Abstract

First, we could succeeded to reconstruct osteocyte network in bone using IMARIS, NEURON TRACER, SURPASS software. We informed the results in international journal "BONE" in 2005. Precise information is in the paper. Briefly, we measured osteocyte morphometry in chick calvaria and also showed morphological differences between osteocytes and osteoblasts. This technique was applied to visualize living osteocytes in bone by using OB7.3 chick osteocyte specific antibody. Now, we are in the way to visualize osteoblasts and osteocytes in living bone.
Besides the morphological differences between osteoblasts and osteocytes, we for the first time demonstrated significant difference between the cells in response to mechanical stress, especially in fluid shear stress. In the study, osteocytes were less respositive to fluid shear stress than osteoblasts. It was believed that osteocytes are the chief mechanosensory cells in bone.
However, in our knowledge based on the present study, osteocyte showed less calcium response than osteoblasts. In addition, we elucidated the implication of different focal adhesion formation between the cells.
Therefore, we now showed morphological and functional differences in osteocytes by the grant this time.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (5 results)

All 2005 Other

All Journal Article (5 results)

  • [Journal Article] Three-dimensional reconstruction of chick calvarial osteocytes and their cell processes using confocal microscopy2005

    • Author(s)
      Sugawara Y, Kamioka H, Honjo T, Tezuka K, Takano-Yamamoto T
    • Journal Title

      Bone 36

      Pages: 877-883

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Three-dimensional reconstruction of chick calvarial osteocytes and their cell processes using confocal microscopy2005

    • Author(s)
      Sugawara Y et al.
    • Journal Title

      Bone 36

      Pages: 877-883

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Fluid shear stress induced less calcium response in a single primary osteocyte than in a single osteoblast; implication of different focal adhesion formation

    • Author(s)
      Kamioka H, Sugawara Y, Murshid SA, Ishihara Y, Honjo T, Takano-Yamamoto
    • Journal Title

      Journal of Bone and Mineral Research (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Fluid shear stress induces less calcium response in a single primary osteocyte than in a single osteoblast : implication of different focal adhesion formation

    • Author(s)
      Kamioka H, Sugawara Y, Murshid SA, Ishihara Y, Honjo T, Takano-Yamamoto
    • Journal Title

      Journal of Bone and Mineral Research (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Three-dimensional reconstruction of chick calvarial osteocytes and their cell processes using confocal microscopy

    • Author(s)
      Sugawara Y, Kamioka H, Honjo T, Tezuka K, Takano-Yamamoto T
    • Journal Title

      Bone (in press)

    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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