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Clarification of the Mechanisms that Causes Internal Derangement of the Temporomandibular Joint

Research Project

Project/Area Number 16390609
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthodontic/Pediatric dentistry
Research InstitutionHealth Sciences University of Hokkaido

Principal Investigator

MIZOGUCHI Itaru  Health Sciences University of Hokkaido, Department of Dentistry, Professor (20200032)

Co-Investigator(Kenkyū-buntansha) TAKUMA Taishin  Health Sciences University of Hokkaido, Department of Dentistry, Professor (40095336)
SASANO Yasuyuki  Tohoku University, Department of Dentistry, Professor (30196191)
飯嶋 雅弘  北海道医療大学, 歯学部, 准教授 (20305915)
OKAYAMA Miki  Health Sciences University of Hokkaido, Department of Dentistry, Assistant (30382500)
Project Period (FY) 2004 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥14,600,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2007: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2006: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 2005: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 2004: ¥7,700,000 (Direct Cost: ¥7,700,000)
KeywordsTemporomandibular joint / Extracellular matrix / Proteoglycans / Collagens / RT-PCR / Western Blotting / 顎関節 / Western Blotting / Western blotting / 顎関節内障 / 顎関節円板 / 軟食摂取 / mRNA / real-time PCR
Research Abstract

To analyse the effects of mechanical environments on gene and protein expressions of major extracellular matrix components in rat temporomandibular joint (TMJ), expression and localization of the core proteins of proteoglycans in the TMJ discs during postnatal development were examined, using Western blotting, immunohistochemistry and rapid-cycle real-time RT-PCR (Real-time Polymerase Chain Reaction). The obtained results were followings; 1. Quantitative RT-PCR showed that mRNA expressions of biglycan and versican were peak after birth and gradually decreased with growing, while decorin mRNA increased with growing. 2. Among four versican isoforms, V0, V1 and V3 decreased with growing, while V2 peaked at 8 weeks and then gradually decreased.
3. Western blotting showed that mRNA expression of rapidly decreased with growing, while mRNA of decorin increased. 4. An immunohistochemical study showed that biglycan was evenly distributed in the TMJ dsics throughout the growing stage. In contrast; localization of decorin and versican showed the regional differences; the stainingfor decorin and biglycan was weak in the central part of the discs and intense in the anterior and posterior peripheral attachments, which would mainly experience tensile forces. These results demonstrate that the growth-related changes and regional differences exist in the expression of proteoglycans in the TMJ discs of growing rats, which probably reflect the alteration of biomechanical environments caused by development of orofacial functions. Further study concerning the effects of food consistency would be necessary to understand mechanisms of temporomandibular joint diseases (TMD)s.

Report

(5 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (40 results)

All 2007 2006 2005 2004

All Journal Article (35 results) (of which Peer Reviewed: 11 results) Presentation (3 results) Book (2 results)

  • [Journal Article] Detection of neurotrophic factors in taste buds by laser capture microdissection, immunohistochemistry, and in situ hybridization2007

    • Author(s)
      Suzuki Y, Mizoguchi I, Uchida N
    • Journal Title

      Arch Histol Cytol 70

      Pages: 117-126

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Detection of neurotrophic factors in taste buds by laser capture microdissection, immunohistochemistry, and in situ hybridization2007

    • Author(s)
      Suzuki, Y., Mizoguchi, I., Uchida, N
    • Journal Title

      Arch Histol Cytol 70

      Pages: 117-126

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] SNAP-23 is not essential for constitutive exocytosis in HeLa cells2007

    • Author(s)
      Okayama M, Arakawa T, Mizoguchi I, Tajima Y, Takuma T
    • Journal Title

      FEBS Lett 581

      Pages: 4583-4588

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ラットの骨、象牙質およびセメント質におけるI型コラーゲンC-プロペプチドの局在2007

    • Author(s)
      富澤 康彦、土谷 昌広、佐藤 繁久、高橋 一郎、土谷 忍、小牧 健一朗、秋葉 陽介、楠慎 一郎、溝口 到、笹野 泰之、渡邉 誠
    • Journal Title

      東北大学歯学雑誌 26

      Pages: 29-36

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression and localization of versican during postnatal development of rat temp oromandibular joint disc2006

    • Author(s)
      Toriya N, Takuma T, Arakawa T, Abiko Y, Sasano Y, Takahashi I, Rahemtulla F, Mizoguchi I
    • Journal Title

      Histochem Cell Biol 125

      Pages: 205-214

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Role of VAMP-2, VAMP-7, and VAMP-8 in constitutive exocytosis from HSY cells2006

    • Author(s)
      Ohishi Y, Takuma M, Mizoguchi I
    • Journal Title

      Histochem Cell Biol 125

      Pages: 273-281

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] One novel and one recurrent mutations in PROS1 gene cause type I pr otein S deficiency in patients with pulmonary embolism associated with deen vein thrombosis2006

    • Author(s)
      Mizukami K, Nakabayashi T, Nai toh S, Takeda M, Tartumi T, M izoguchi I, Ieko M, Koike T
    • Journal Title

      Amer J Hematol 81

      Pages: 787-797

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Expression and localization of versican during postnatal development of rat temporomandibular joint disc2006

    • Author(s)
      Toriya, N., Takuma, T., Arakawa, T., Abiko, Y., Sasano, Y., Takahashi, I., Rahemtulla, F., Mizoguchi, I
    • Journal Title

      Histochem Cell Biol 125

      Pages: 205-214

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Role of VAMP-2, VAMP-7, and VAMP-8 in constitutive exocytosis from HSY cells2006

    • Author(s)
      Ohishi, Y., Takuma, M., Mizoguchi, I
    • Journal Title

      Histochem Cell Biol 125

      Pages: 273-281

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] One novel and one recurrent mutations in PROS1 gene cause type I protein S deficiency in patients with pulmonary embolism associated with deep vein thrombosis2006

    • Author(s)
      Mizukami, K., Nakabayashi, T., Naitoh, S., Takeda, M., Tatumi, T., Mizoguchi, I., Ieko, M., Koike, T
    • Journal Title

      Amer J Hematol 81

      Pages: 787-797

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] 実験的歯の異動時のラット歯周組織圧迫側におけるMMP-13の局在2006

    • Author(s)
      水上和博, 浜谷明里, 坂倉康則, 矢嶋俊彦, 水口到
    • Journal Title

      北海道医療大学歯学会雑誌 25(1)

      Pages: 53-62

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 株下骨芽細胞様細胞MC3T3-E1細胞の分化・石灰化過程におけるデコリンとバイグリカンのmRA発現2006

    • Author(s)
      泰間康平, 鳥谷奈保子, 荒川俊哉, 田隈到
    • Journal Title

      北海道医療大学歯学会雑誌 25(1)

      Pages: 63-72

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Galvalic corrosin behavior of orthodontic archwire alloys coupled to bracket alloys.2006

    • Author(s)
      iijima M, Endo K, Yuasa T, Ohno H, Hayashi K, Mizoguchi I
    • Journal Title

      Angle Orthod 76(4)

      Pages: 705-711

    • Related Report
      2006 Annual Research Report
  • [Journal Article] One nobel and one recurrento mutations in PROS1 gene cause type I protein S deficiency in patients with pulmonay embokism associated with dep vein thrombosis.2006

    • Author(s)
      Mizukami K, Nakabayashi T, Naitoh S, Takeda Mhosi Tartumi T, Mizoguchi K, Ieiko M, Koike T
    • Journal Title

      Amer J Hematol 81(10)

      Pages: 787-797

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Comparison of the helical axis and rectangular coordinate system in representing orthoudontic tooth movement.2006

    • Author(s)
      Hayashi K, Delong R, Mizoguchi I
    • Journal Title

      J Biomechanics 39(16)

      Pages: 2955-2933

    • Related Report
      2006 Annual Research Report
  • [Journal Article] A nobel method for three-dimensional(3-D) simulation of orthognathic surgery using a multimodal image fusion technique.2006

    • Author(s)
      Uechi J, Okayama M, Shibata T, Muguruma T, Hayashi K, Endo K, Mizoguchi I
    • Journal Title

      Amer J Orthod Dentofacial Orthop In press

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Expression and localization of versican during postnatal development of rat temporomandibular joint disc2006

    • Author(s)
      Toriya N, Takuma T, Arakawa T, Abiko Y, Sasano Y, Takahashi I, Rahemtulla F, Mizoguchi I
    • Journal Title

      Histochem Cell Biol 125

      Pages: 205-214

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Role of VAMP-2, VAMP-7, and VAMP-8 in constitutive exocytosis from HSY cells2006

    • Author(s)
      Ohishi Y, Arakawa T, Tanimura A, Itakura M, Takahashi M, Mizoguchi I, Takuma M
    • Journal Title

      Histochem Cell Blot 125

      Pages: 273-281

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 成長期ラット顎関節円板におけるversican mRNAの発現-taqMan probeを用いたReal-time quantitative PCRによる分析2005

    • Author(s)
      鳥谷 奈保子、荒川 俊哉、田隈 泰信、安彦 善裕、溝口 到
    • Journal Title

      北海道医療大学歯学会雑誌 24

      Pages: 223-235

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Protein C Sapporo(protein C Glu 25 -> Lys): a heterozygous missense mutati on in the Gla domain provides new insight into the interaction between protein C and endothelial protein C receptor2005

    • Author(s)
      Nakabayashi T, Mizukami K, Naitoh S, Takeda M, Shikamoto Y, Nakag awa T, Kaneko H, Tartumi T, Mizo guchi I, Mizuno H, Ieko M, Koike T
    • Journal Title

      Thromb Haemost 94

      Pages: 942-950

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Expression of versican and ADAMTS during rat tooth eruption2005

    • Author(s)
      Sone S, Nakamura M, Maruya Y, T akahashi I, Mizoguchi I, mayanagi H, Sasano Y
    • Journal Title

      Mol Histol 36

      Pages: 281-288

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Expression of versican and ADAMTS1, 4, and 5 during bone development in the rat mandible and hind limb2005

    • Author(s)
      Nakamura M, Sone S, Takahashi I, Mizoguchi I, Echigo S, Sasano Y
    • Journal Title

      J Histochem Cytochem 53

      Pages: 1553-1562

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] mRNA expression of versican in the temporomandibular joint dsik of growing rats2005

    • Author(s)
      Toriya, N., Arakawa, T., Takuma, T., Abiko, Y., Mizoguchi, I
    • Journal Title

      J Dent Health Sci Univ 24

      Pages: 223-235

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Protein C Sapporo(protein C Glu 25→ Lys) : a heterozygous missense mutation in the Gla domain provides new insight into the interaction between protein C and endothelial protein C receptor2005

    • Author(s)
      Nakabayashi, T., Mizukami, K., Naitoh, S., Takeda, M., Shikamoto, Y., Nakagawa, T., Kaneko, H., Tartumi, T., Mizoguchi, I., Mizuno, H., leko, M., Koike, T
    • Journal Title

      Thromb Haemost 94

      Pages: 942-950

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Expression of versican and ADAMTS during rat tooth eruption2005

    • Author(s)
      Sone, S., Nakamura, M., Maruya, Y., Takahashi, I., Mizoguchi, I., mayanagi, H., Sasano, Y
    • Journal Title

      Mol Histol 36

      Pages: 281-288

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Expression of versican and ADAMTS1, 4, and 5 during bone development in the rat mandible and hind limb2005

    • Author(s)
      Nakamura, M., Sone, S., Takahashi, I., Mizoguchi, I., Echigo, S., Sasano, Y
    • Journal Title

      J Histochem Cytochem

      Pages: 1553-1562

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] 成長期ラット顎関節円板におけるversican mRNAの発現-taqMan probeを用いたReal-time quantitative PCRによる分析-2005

    • Author(s)
      鳥谷奈保子, 荒川俊哉, 田隈泰信, 安彦善裕, 溝口 到
    • Journal Title

      北海道医療大学歯学会雑誌 24

      Pages: 223-235

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Expression of versican and ADAMTS during rat tooth eruption2005

    • Author(s)
      Sone S, Nakamura M, Maruya Y, Takahashi I, Mizoguchi I, Mayanagi H, Sasano Y
    • Journal Title

      J Mol Histol 36

      Pages: 281-288

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Expression of versican and ADAMTS1, 4 and 5 during bone development in the rat mandible and hind limb2005

    • Author(s)
      Nakamura M, Sone S, Takahashi I, Rahemtulla F, Mizoguchi I, Echigo S, Sasano Y
    • Journal Title

      J Histochem Cytochem 53

      Pages: 1553-1562

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Simulation study for a finite helical axis(FHA) analysis of tooth movement2005

    • Author(s)
      Hayashi K, Hamaya M, Mizoguchi I
    • Journal Title

      Angle Orthod 75(3)

      Pages: 328-333

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Signaling mechanisms involved in protease-activated receptor-l-mediated interleu kin-6 production by human gingival fibroblasts2004

    • Author(s)
      Tanaka N, Morita T, Nezu A, Tani mura A, Mizoguchi I, Tojyo Y
    • Journal Title

      J Pharmacol Exp Ther 311

      Pages: 778-786

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Signaling mechanisms involved in protease-activated receptor-1- mediated interleukin-6 production by human gingival fibroblasts2004

    • Author(s)
      Tanaka, N., Morita, T., Nezu, A., Tanimura, A., Mizoguchi, I., Tojyo, Y
    • Journal Title

      J Pharmacol Exp Ther 311

      Pages: 778-786

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Signaling mechanisms involved in PAR-1-mediated interleukin-6 production by gingival fibroblasts2004

    • Author(s)
      Tanaka N, Morita T, Nezu A, Tanimura A, Mizoguchi I, et al.
    • Journal Title

      J Pharmacol Exp Ther 311(2)

      Pages: 778-786

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Basic behavior of the finite helical axis in a simple tooth movement simulation2004

    • Author(s)
      Hayashi K, Tanaka H, Hikita K, Mizoguchi I
    • Journal Title

      Med Eng Phys 26(10)

      Pages: 867-872

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Comparison of maxillary canine retraction with sliding mechanics and a retraction spring2004

    • Author(s)
      Hayashi K, Uechi J, Murata M, Mizoguchi I
    • Journal Title

      Eur J Orthod 26(6)

      Pages: 585-589

    • Related Report
      2004 Annual Research Report
  • [Presentation] 重力負荷はMC3T3-E1細胞において2つのシグナル経路を介してERKをリン酸化する2007

    • Author(s)
      荒川 俊哉、岡山 三紀、安彦 善裕、溝口 到、田隈 泰信
    • Organizer
      第49回歯科基礎医学医会学術大会
    • Place of Presentation
      仙台
    • Related Report
      2007 Annual Research Report
  • [Presentation] 成長期ラット顎関節円板におけるsmallleucinerichproteoglycanのmRNA発現2005

    • Author(s)
      甲田 尚央、鳥谷 奈保子、荒川 俊哉、田隈 泰信、笹野 泰之、溝口 到
    • Organizer
      第65回日本矯正歯科学会
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] mRNA expression of small leusine-rich proteoglycans in the temporomandibular joint disc of growing rats2005

    • Author(s)
      Kohda, N., Toriya, N., Arakawa, T., Takuma, T., Sasano, Y., Mizoguchi, I
    • Organizer
      Congress of Japanese Orthodontic Society
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] 歯根吸収の危険因子.歯の移動の臨床バイオメカニク2006

    • Author(s)
      溝口 到
    • Total Pages
      10
    • Publisher
      医歯薬出版
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] 臨床 : 歯根吸収の危険因子.歯の異動の臨床バイオメカニクス2006

    • Author(s)
      講口 到(編集および執筆)
    • Total Pages
      10
    • Publisher
      医歯薬出版
    • Related Report
      2006 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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