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Molecular Epidemiology of Trypanosoma evansi

Research Project

Project/Area Number 16405035
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section海外学術
Research Field Applied veterinary science
Research InstitutionObihiro University of Agriculture and Veterinary Medicine

Principal Investigator

INOUE Noboru  National Research Center for Protozoan Diseases, Associate Professor, 原虫病研究センター, 助教授 (10271751)

Co-Investigator(Kenkyū-buntansha) XUAN Xuenan  Obihiro University of Agriculture and Veterinary Medicine, National Research Center for Protozoan Diseases., Professor, 原虫病研究センター, 教授 (10292096)
SUGIMOTO Chihiro  Hokkaido University, Research Center for Zoonosis Control., Professor, 人獣共通感染症リサーチセンター, 教授 (90231373)
Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥12,500,000 (Direct Cost: ¥12,500,000)
Fiscal Year 2006: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2005: ¥4,200,000 (Direct Cost: ¥4,200,000)
Fiscal Year 2004: ¥6,600,000 (Direct Cost: ¥6,600,000)
KeywordsTrypanosoma evansi / Asia / Epidemiology / LAMP / トリパノソーマ エバンシ / 診断 / 疫学 / アフリカ
Research Abstract

Recently we have developed a new diagnostic method for Trypanosoma evansi infection based on isothermal DNA amplification method, called LAMP (loop-mediated isothermal amplification of DNA). Since the method is highly sensitive, specific, and easy-to-use, we applied this method for epidemiological study of Surra, T evansi infection in Asian countries where T. evansi infection is endemic. We have collected field samples from Cattle and buffaloes in China, Mongolia, India, Philippines, and Eastern Afirica. In northern China and Mongolia, infection rate of T. evansi is less than 5%. However, the infection rate is very high (>50%) in western China. Therefore, environmental factors, such as climate, vector population, seems to affect epidemiology of T. evansi infection. We have also done detection of trypanosomes from tsetse fly because our LAMP method can be applicable for both animal and insect derived samples.
In order to improve sensitivity and specificity of our LAMP method, 2 major problems have to be solved. First problem is an occasional false positive reaction in LAMP reaction. Second problem is complicated DNA preparation procedures for template DNA preparation from biological samples. To solve those problems, we are now going on the another research project supported by Foundation for Innovative New Diagnostics (FIND).
It is noteworthy that our research institute (National Research Center for Protozoan Diseases) will be an OIE reference laboratory for T. evansi infection, bovine and equine piroplasmosis from May 2007. By this condition, we have to make considerable efforts on improvement of our LAMP method.

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (28 results)

All 2007 2006 2005 2004

All Journal Article (28 results)

  • [Journal Article] Preliminary application and evaluation of loop-mediated isothermal amplification (LAMP) for detection of bovine theileriosis and trypanosomosis in Tanzania2007

    • Author(s)
      Thekisoe, O. M. M.
    • Journal Title

      Onderstepoort Journal of Veterinary Research (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Comparative evaluation of the sensitivity of LAMP, PCR and in vitro culture methods for the diagnosis of equine piroplasmosis2007

    • Author(s)
      Alhassan, A.
    • Journal Title

      Parasitology Research 100・5

      Pages: 1165-1168

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Effects of exogenous transforming growth factor-beta on Trypanosoma congolense infection in mice2007

    • Author(s)
      Namangala, B.
    • Journal Title

      Infection and Immunity 75・4

      Pages: 1878-1885

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] A new trypanosoma species isolated from naturally infected Haemaphysalis hystricis ticks in Kagoshima Prefecture, Japan2007

    • Author(s)
      Thekisoe, O. M. M.
    • Journal Title

      Parasitology 19・1

      Pages: 1-8

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Haemaphysalis longicornis : Molecular characterization of a homologue of the macrophage migration inhibitory factor from the partially fed ticks2007

    • Author(s)
      Umemiya, R.
    • Journal Title

      Experimental Parasitology 115・2

      Pages: 135-142

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Development of loop-mediated isothermal amplification (LAMP) method for diagnosis of equine piroplasmosis2007

    • Author(s)
      Alhassan, A.
    • Journal Title

      Veterinary Parasitology 143・2

      Pages: 155-160

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Preliminary application and evaluation of loop-mediated isothermal amplification (LAMP) for detection of bovine theileriosis and trypanosomosis in Tanzania2007

    • Author(s)
      Thekisoe, O.M.M.
    • Journal Title

      Onderstepoort Journal of Veterinary Research (In Press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Comparative evaluation of the sensitivity of LAMP, PCR and in vitro culture methods for the diagnosis of equine piroplasmosis2007

    • Author(s)
      Alhassan, A.
    • Journal Title

      Parasitology Research 100

      Pages: 1165-1168

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effects of exogenous transforming growth factor-beta on Trypanosoma congolense infection in mice2007

    • Author(s)
      Namangala, B.
    • Journal Title

      Infection and Immunity 75

      Pages: 1878-1885

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A new trypanosoma species isolated from naturally infected Haemaphysalis hystricis ticks in Kagoshima Prefecture, Japan2007

    • Author(s)
      Thekisoe, O. M. M.
    • Journal Title

      Parasitology 19

      Pages: 1-8

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Haemaphysalis longicornis : Molecular characterization of a homologue of the macrophage migration inhibitory factor from the partially fed ticks2007

    • Author(s)
      Umemiya, R.
    • Journal Title

      Experimental Parasitology 115

      Pages: 135-142

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Development of loop-mediated isothermal amplification (LAMP) method for diagnosis of equine piroplasmosis2007

    • Author(s)
      Alhassan, A.
    • Journal Title

      Veterinary Parasitology 143

      Pages: 155-160

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Species-specific loop-mediated isothermal amplification (LAMP) for diagnosis of trypanosomosis2007

    • Author(s)
      Thekisoe, O. M. M.
    • Journal Title

      Acta Tropica (印刷中)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Evidence for the immunostimulatory effects of low-dose orally-delivered human interferon alpha in cattle2006

    • Author(s)
      Namangala, B.
    • Journal Title

      Journal of Interferon and Cytokine Research 26・9

      Pages: 675-681

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Identification and characterization of a leucine aminopeptidase from the hard tick Haemaphysalis longicornis2006

    • Author(s)
      Hatta, T.
    • Journal Title

      International Journal of Parasitology 36・10-11

      Pages: 1123-1132

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Beneficial effect of erythropoietin administration on murine infection with Trypanosoma congolense2006

    • Author(s)
      Suzuki, T.
    • Journal Title

      The American Journal of Tropical Medicine and Hygiene 74・6

      Pages: 1020-1025

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Efficacy of dipalmitoylphosphatidylcholine liposome against African trypanosomes2006

    • Author(s)
      Kuboki, N.
    • Journal Title

      Journal of Parasitology 92・2

      Pages: 389-393

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Evidence for the immunostimulatory effects of low-dose orally-delivered human interferon alpha in cattle2006

    • Author(s)
      Namangala, B.
    • Journal Title

      Journal of Interferon and Cytokine Research 26

      Pages: 675-681

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Identification and characterization of a leucine aminopeptidase from the hard tick Haemaphysalis longicornis2006

    • Author(s)
      Hatta, T.
    • Journal Title

      International Journal of Parasitology 36

      Pages: 1123-1132

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Beneficial effect of erythropoietin administration on murine infection with Trypanosoma congolense2006

    • Author(s)
      Suzuki, T.
    • Journal Title

      The American Journal of Tropical Medicine and Hygiene 74

      Pages: 1020-1025

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Efficacy of dipalmitoylphosphatidylcholine liposome against African trypanosomes2006

    • Author(s)
      Kuboki, N.
    • Journal Title

      Journal of Parasitology 92

      Pages: 389-393

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Efficacy of dipalmitoylphosphatidylcholine liposome against African typanosomes.2006

    • Author(s)
      Kuboki N. et al.
    • Journal Title

      Journal of Parasitology 92・2(印刷中)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Genetic variability in ESAG6 genes among Trypanosoma evansi isolates and in comparison to other Trypanozoon members.2005

    • Author(s)
      Witola, W.H. et al.
    • Journal Title

      Acta Tropica 93・1

      Pages: 67-73

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Evaluation of loop-mediated isothermal amplification (LAMP), PCR and parasitological tests for detection of Trypanosoma evansi in experimentally infected pigs.2005

    • Author(s)
      Thekisoe, O.M.M. et al.
    • Journal Title

      Veterinary Parasitology 130・3-4

      Pages: 327-330

    • Related Report
      2005 Annual Research Report
  • [Journal Article] トリパノソーマエバンシ実験感染豚由来血液を用いたLAMP法の評価2005

    • Author(s)
      オリエル テキソエ
    • Journal Title

      獣医寄生虫学会誌 3

      Pages: 62-62

    • Related Report
      2004 Annual Research Report
  • [Journal Article] トリパノソーマコンゴレンセ特異的診断法の開発2005

    • Author(s)
      ワレン バティカドス
    • Journal Title

      獣医寄生虫学会誌 3

      Pages: 63-63

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Expression of a gene encoding Trypanosoma congolense putative Abc1 family protein is developmentally regulated.2005

    • Author(s)
      Waren N.Baticados
    • Journal Title

      The Journal of Veterinary Medical Science 67

      Pages: 157-164

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 組換えリボソームPO蛋白質を用いたトリパノソーマ特異的ELISA法の開発2004

    • Author(s)
      櫻井 達也
    • Journal Title

      獣医寄生虫学会誌 3

      Pages: 58-58

    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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