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Elucidation of the cause of Caribbean parkinsonism and prevention in other area

Research Project

Project/Area Number 16406004
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section海外学術
Research Field Environmental pharmacy
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

OHTA Shigeru  Hiroshima University, Graduate School of Biomedical Sciences, Professor, 大学院・医歯薬学総合研究科, 教授 (60160503)

Co-Investigator(Kenkyū-buntansha) KOTAKE Yaichiro  Hiroshima University, Graduate School of Biomedical Sciences, Research Associate, 大学院・医歯薬学総合研究科, 助手 (20335649)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥13,000,000 (Direct Cost: ¥13,000,000)
Fiscal Year 2005: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2004: ¥9,900,000 (Direct Cost: ¥9,900,000)
Keywordsbenzylisoguinoline / Parkinson's disease / reticuline / N-methylcoculaurine / LC / MS / French West Indies / Annonaceae family
Research Abstract

In Guaderoupe, the French West Indies, there is a high incidence of atypical parkinsonism or progressive supranuclear palsy, and all of the investigated patients had taken herbal tea or tropical fruits of the Annonaceae family. Local inhabitants consume the fruits, and also drink tea made from the leaves. In the present study, we used liquid chromatography-tandem mass spectrometry (LC/MS/MS) with multiple reaction monitoring (MRM) to detect low-molecular-weight neurotoxic benzylisoquinoline derivatives in the Annonaceae family. We detected reticuline and N-methylcoculaurine in every Annona muricata sample examined, except for pulp and seed. They were not detected in sweetsop fruits. Norreticuline was not detected in any sample. These three compounds were toxic to SH-SY5Y neuroblastoma cells and inhibited mitochondrial respiratory complex I. It is possible that uptake of the benzylisoquinoline derivatives reticuline and N-methylcoculaurine and their accumulation in the brain may be related to the pathogenesis of the local endemic disease.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (10 results)

All 2006 2005 2004

All Journal Article (10 results)

  • [Journal Article] Glutamate excitotoxicity is involved in cell death caused by tributyltin in cultured rat cortical neurons.2006

    • Author(s)
      Nakatsu Y. et al.
    • Journal Title

      Toxicol.Sci. 89

      Pages: 235-242

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Tributyltin-induced cell death is mediated by calpain in PC 12 cells.2006

    • Author(s)
      Nakatsu Y. et al.
    • Journal Title

      Neurotoxicology (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Parkinsonism-preventing activity of 1-methyl-1,2,3,4-tetrahydroisoquinoline derivatives in C57BL mouse in vivo.2006

    • Author(s)
      Okuda K. et al.
    • Journal Title

      Biol.Pharm.Bull. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Neuroprotective effect of 1-methyl-1,2,3,4-tetrahydroisoquinoline on cultured rat mesencephalic neurons in the presence or absence of various neurotoxins2005

    • Author(s)
      Y.Kotake et al.
    • Journal Title

      Brain Res. 1033

      Pages: 143-150

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Glutamate excitotoxicity is involved in cell death caused by tributyltin in cultured rat cortical neurons2005

    • Author(s)
      Y.Nakatsu et al.
    • Journal Title

      Toxicol. Sci. 89

      Pages: 235-242

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Neuroprotective effect of 1-methyl-1,2,3,4-tetrahydroisoquinoline on cultured rat mesencephalic neurons in the presence or absence of various neurotoxins.2005

    • Author(s)
      Kotake Y. et al.
    • Journal Title

      Brain Res. 1033

      Pages: 143-150

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Glutamate excitotoxicity is involved in cell death caused by tributyltin in cultured rat cortical neurons2005

    • Author(s)
      Y.Nakatsu et al.
    • Journal Title

      Toxicol.Sci. 89

      Pages: 235-242

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Neuroprotective effect of 1-methyl-1,2,3,4-tetrahydroisoquinoline on cultured rat mesencephalic neurons in the presence or absence of various neurotoxins.2005

    • Author(s)
      Y.Kotake et al.
    • Journal Title

      Brain Res. 1033

      Pages: 143-150

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Detection and determination of reticuline and N-methylcoculaurine in the Annonaceae family using liquid chromatography-tandem mass spectrometry.2004

    • Author(s)
      Kotake Y. et al.
    • Journal Title

      Chromatography B. 806

      Pages: 75-78

    • NAID

      10016551931

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Detection and determination of reticuline and N-methylcoculaurine in the Annonaceae family using liquid chromatography-tandem mass spectrometry.2004

    • Author(s)
      Y.Kotake, et al.
    • Journal Title

      J.Chromatogr.B 806

      Pages: 75-78

    • NAID

      10016551931

    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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