| Project/Area Number |
16406026
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 海外学術 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | University of Fukui |
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Principal Investigator |
ISHIZAKI Takeshi University of Fukui, Faculty of Medicine, Professor, 医学部, 教授 (80151364)
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| Co-Investigator(Kenkyū-buntansha) |
AMESHIMA Shingo University of Fukui Hospital, Lecturer, 医学部附属病院, 講師 (60262614)
MATSUKAWA Shigeru University of Fukui, Centers for Advanced Research Support, Associate Professor, 助教授 (00092809)
SAKAI Akio Shinshu University, Graduate School of Medicine, Associate Professor, 大学院医学研究科, 助教授 (70020758)
MIZUNO Shirou University of Fukui, Faculty of Medicine, Assistant Professor, 医学部, 助手 (80397281)
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| Project Period (FY) |
2004 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥12,600,000 (Direct Cost: ¥12,600,000)
Fiscal Year 2006: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥4,400,000 (Direct Cost: ¥4,400,000)
Fiscal Year 2004: ¥4,700,000 (Direct Cost: ¥4,700,000)
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| Keywords | Yak / Pulmonary Circulation / eNOS / DDAH / ADMA / ET-1 / 低酸素適応 / 高地適応 / 極限高地生息動物 / ナキウサギ / HIF1α / ET1 |
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| Research Abstract |
We investigated the pulmonary characteristics of Yak, a high-altitude adapted animal that is resistant to hypoxia-induced pulmonary hypertension, cor pulmonale in terms of hypoxia-responsible genomic products such as eNOS, DDAH (dimethylarginine dimethylaminohydrolase), ADMA (asymmetrically methylated Arginine) and ET-1.
Results;
1. The protein expression of eNOS, and protein expression and activity of DDAH in yak's lung tissue were more elevated than that of control cow. However, the degree of protein expression of DDAH in the Kidney was the same in both animals.
2. The plasma level of ADMA was 5-times higher in Yak compared to that of cow. Among cow groups, high-altitude living one has 4-times high ADMA level compared to that of low-altitude living one.
3. The plasma ET-1 level was higher in Yak than in cow.
These results suggest that wild yak's lung has augmented eNOS protein and augmented DDAH activity compared to cow. On the contrary, the animal has elevated plasma ADMA and ET-1 suggesting that the balance of vasodilators and vasoconstrictors are up-regulated. Although we did not assess transpulmonary difference of plasma ADMA and ET-1, it appears that Yak's lung preferentially expressed protective NO mechanism(s) which may result the relatively low pulmonary arterial pressure despite of high-altitude. It may induce a good suggestion from the animal study if think about hypoxic-air induced pulmonary hypertension and chronic respiratory failure in clinical medicine in terms of pathogenesis and treatment strategy.
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