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Molecular biologic and pathologic analysis of Epstein-Barr virus infection related salivary lymphoepithelial carcinoma

Research Project

Project/Area Number 16406033
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section海外学術
Research Field Morphological basic dentistry
Research InstitutionNiigata University

Principal Investigator

CHENG Jun  Niigata University, Institute of Medicine and dentistry, Associate Professor, 医歯学系, 助教授 (40207460)

Co-Investigator(Kenkyū-buntansha) MARUYAMA Satoshi  Niigata University, Institute of Medicine and dentistry, Assistant, 医歯学系, 助手 (30397161)
SAKU Takashi  Niigata University, Institute of Medicine and dentistry, Professor, 医歯学系, 教授 (40145264)
依田 浩子  新潟大学, 大学院・医歯学総合研究科, 助手 (60293213)
大城 和文  新潟大学, 大学院・医歯学総合研究科, 助手 (50332648)
Project Period (FY) 2004 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥11,300,000 (Direct Cost: ¥11,300,000)
Fiscal Year 2006: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 2005: ¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 2004: ¥6,700,000 (Direct Cost: ¥6,700,000)
Keywordslymphoepithelial carcinoma / salivary gland / EBV / LMP1 gene / cell culture / cloning / lymphoepithelial cyst / benign lymphoepithelial lesion / リンパ上皮性癌 / 中国 / 遺伝子導入 / 塩基配列決定 / NF-kB
Research Abstract

We have collected the total 180 cases of lymphoepithelial carcinoma (LEC) of salivary gland, which were confirmed as EBV-infected tumors by various molecular biological.
A complete sequence for the LMP1 gene, one of the EBV gene and has been regarded as an oncogene, was obtained from patients' tissue samples. It was cloned into an expression vector and the constructs were transfected into 293T cells. The transfected cells showed significant elevation of NFkB activities and suppressed cell growth, which were also seen in cells transfected with vectors with different LMP1 genes such as CAO, which has been isolated from a nasopharyngeal carcinoma, and B95-8, a prototype EBV. The results indicated that the expression of LMP1 affects transcription activities in LEC cells but that the overall mutations found in the present study were not always reasons for the tumorigenesis. The promoter region of the LMP1 gene was also analyzed from 20 cases of them, and all of the cases shared common mutational events in regions that were expected to be associated with transcription factors.
We tried primary cultures for salivary LECs for many times. Unfortunately, however, it was hard to establish cell lines from the cells in culture. In one of the primary cultures, we were successful in maintaining cells, which were confirmed to be their salivary duct epithelial and myoepithelial characteristics as well as EBV infected.
As a background disease, lymphoepithelial cyst was studied based on clinicopathological analyses of sixty-four cases of the parotid gland. The lymphoid stroma of the cyst seemed to be resulted from granulation tissue reaction of focal sialadenitis but not induced by EBV infection.
Our functional study shows a new insight for EBV roles in the pathogenesis of salivary LEC, although it is still necessary to carry out further investigations for the whole mechanisms of EBV oncogenesis.

Report

(4 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • Research Products

    (21 results)

All 2007 2006 2005 2004

All Journal Article (21 results)

  • [Journal Article] Lymphatic involvement in the histopathogenesis of mucous retention cyst2007

    • Author(s)
      Kundu S
    • Journal Title

      Pathol Res Pract 203(2)

      Pages: 89-97

    • Related Report
      2006 Annual Research Report
  • [Journal Article] A clinicopathologic study of parotid gland lymphoepithelial cyst2006

    • Author(s)
      Wu L
    • Journal Title

      Oral Med Pathol 11(4)

      Pages: 40-40

    • Related Report
      2006 Annual Research Report
  • [Journal Article] A clinicopathologic study of parotid gland lymphoepithelial cyst.2006

    • Author(s)
      Wu LY
    • Journal Title

      Oral Med & Pathol 11(1)(In press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Nucleotide sequences and functions of the Epstein-Barr virus latent membrane protein 1 genes isolated from salivary gland lymphoepithelial carcinomas.2005

    • Author(s)
      Jen KY
    • Journal Title

      Virus Genes 30(2)

      Pages: 223-235

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Primary cultures of Epstein-Barr virus-related lymphoepithelial carcinoma cells of the salivary gland.2005

    • Author(s)
      Cheng J
    • Journal Title

      Korean J Oral Maxillofacial Pathol 29(2)

      Pages: 169-169

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Nucleotide sequences and functions of the epstein-barr virus latent membrane protein 1 genes isolated from salivary gland lymphoepithelial carcinomas2005

    • Author(s)
      Jen KY, Higuchi M, Cheng J, Li J, Wu LY, Li YF, Lin HL, Chen Z, Gurtsevitch V, Fujii M, Saku T
    • Journal Title

      Virus Genes 30(2)

      Pages: 223-235

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Primary cultures of Epstein-Barr virus-related lymphoepithelial carcinoma cells of the salivary gland2005

    • Author(s)
      Cheng J, Jen KY, Zhou X, Tian Z, Maruyama S, Li J, Saku
    • Journal Title

      Korean J Oral Maxillofacial Pathol 29(2)

      Pages: 169-169

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Nucleotide sequences and functions of the Epstein-Barr virus latent membrane protein 1 genes isolated from salivary gland lymphoepithelial carcinomas.2005

    • Author(s)
      Jen Y
    • Journal Title

      Virus Genes 30(2)

      Pages: 223-235

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Salivary lymphoepithelial carcinoma cells in culture : evidence for EBV infection2005

    • Author(s)
      Cheng J
    • Journal Title

      Proceedings of 2005-Sino-Japanese Conference on Stomatology

      Pages: 50-51

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Molecular pathology of salivary lymphoepithelial carcinomas with EBV infection : China-Japan collaboration research for ten years2005

    • Author(s)
      Saku T
    • Journal Title

      東北三省口腔医学学術会議論文編

      Pages: 3-4

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Primary cultures of Epstein-Barr virus-related lymphoepithelial carcinoma cells of the salivary gland2005

    • Author(s)
      Cheng J
    • Journal Title

      Korean Journal of Oral and Maxillofacial Pathology 29(2)

      Pages: 169-169

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 口蓋腫瘍2005

    • Author(s)
      程 くん
    • Journal Title

      日本病理学会東北支部学術集会抄録集症例データベース http://www.jsptn.mcpu.jp/index.html

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Nucleotide sequences and functions of the Epstein-Barr virus latent membrane protein 1 genes isolated from salivary gland lymphoepithelial carcinomas2005

    • Author(s)
      Jen KY
    • Journal Title

      Virus Genes 30(2)

      Pages: 223-235

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Epstein-Barr virus infection in benign lymphoepithelial lesions with malignant transformation of salivary glands.2004

    • Author(s)
      Wu LY
    • Journal Title

      Zhonghua Kou Qiang Yi Xue Za Zhi 39(2)

      Pages: 291-293

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Epstein-Barr virus infection in lymphoepithelial carcinoma of salivary glands in Sichuan Chinese2004

    • Author(s)
      Wu LY
    • Journal Title

      Sichuan Da Xue Xue Bao Yi Xue Ban 35(4)

      Pages: 506-507

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Benign lymphoepithelial lesion of minor salivary gland2004

    • Author(s)
      Metwaly H
    • Journal Title

      Oral Oncology Extra 40(10)

      Pages: 113-116

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Epstein-Barr virus infection in benign lymphoepithelial lesions with malignant transformation of salivary glands2004

    • Author(s)
      Wu LY, Cheng J, Lu Y, Zhou ZY, Saku T
    • Journal Title

      Zhonghua Kou Qiang Yi Xue Za Zhi 39(4)

      Pages: 291-293

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Epstein-Barr virus infection in lymphoepithelial carcinoma of salivary glands in Sichuan Chinese2004

    • Author(s)
      Wu LY, Cheng J, Lu Y, Zhou ZY, Saku T
    • Journal Title

      Sichuan Da Xue Xue Bao Yi Xue Ban 35(4)

      Pages: 506-507

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Benign lymphoepithelial lesion of minor salivary gland2004

    • Author(s)
      Metwaly H, Cheng J, Suzuki M, Takata Y, Saito C, Hayashi T, Saku T
    • Journal Title

      Oral Oncology Extra 40(10)

      Pages: 113-116

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Epstein-Barr virus infection in benign lymphoepithelial lesions with malignant transformation of salivary glands2004

    • Author(s)
      Wu LY
    • Journal Title

      Zhonghua Kou Qiang Yi Xue Za Zhi 39(4)

      Pages: 291-293

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Benign lymphoepithelial lesion of minor salivary gland : report of a case involving the palatal mucosa2004

    • Author(s)
      Metwaly H
    • Journal Title

      Oral Oncology Extra 40(10)

      Pages: 113-116

    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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