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cDNA cloning and endogenous ligand identification of the novel ligand-gated ion-channel receptors

Research Project

Project/Area Number 16500231
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Nerve anatomy/Neuropathology
Research InstitutionKansai Medical University

Principal Investigator

HOUTANI Takeshi  Kansai Medical University, Faculty of Medicine, Assistant Professor, 医学部, 講師 (60241163)

Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2005: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 2004: ¥600,000 (Direct Cost: ¥600,000)
Keywordsserotonin / ion channel / human genome / gene expression / RT-PCR
Research Abstract

5HT3C is the new member of serotonin-gated ionotropic receptor. From the sequence homology search for 5HT3C cDNA against human genomic database, three additional receptor genes, designated 5HT3D, 3E and 3L1, were identified on the same chromosome 3q27.1. These four predicted genes are composed of nine exons and share common features in exon-intron organization with previously known serotonin receptors 5HT3A and 3B. RT-PCR analysis in human tissues demonstrated that 5HT3C mRNA was highly expressed in placenta, lung and kidney, whereas 5HT3D mRNA was seen in lung and pancreas. The cDNA cloning revealed that alternative splicing form lacking exon 2,3,4 and 6 of 5HT3C and one lacking exon 3 of 5HT3D were expressed in kidney and pancreas, respectively. To compare the expression levels of these mRNA in each tissue, PCR-RFLP assay was performed by using common primers designed from the conserved sequences in exon 7 and exon 9. In brain, lung and liver, 5HT3C transcript was dominant, while 5HT3D message was mainly expressed in heart. Coexpression of 5HT3D and 5HT3L1 was demonstrated in skeletal muscle while 5HT3C, 5HT3D and 5HT3L1 were co-expressed in placenta, kidney and pancreas. The expression of 5HT3E transcript could not be detected, and hence, this gene was assumed as a pseudogene. Furthermore, other splicing variants at the junction of exon 7 and exon 8 of 5HT3D and 5HT3L1 were expressed in pancreas. Taken together, these results suggest that novel identified ion-channel receptors execute or modulate many physiological functions in diverse tissues through oligomerization of various splicing variants.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (10 results)

All 2005 2004

All Journal Article (10 results)

  • [Journal Article] Cloning and expression of ligand-gated ion-channel receptor L2 in central nervous system.2005

    • Author(s)
      Takeshi Houtani
    • Journal Title

      Biochem. Biophys. Res. Commun. 335(2)

      Pages: 277-285

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Enhanced hippocampal acetylcholine release in nociceptin-receptor knockout mice.2005

    • Author(s)
      Kayoko Uezu
    • Journal Title

      Brain Res. 1050(1-2)

      Pages: 118-123

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Cloning and expression of ligand-gated ion-channel receptor L2 in central nervous system.2005

    • Author(s)
      Takeshi Houtani, et al.
    • Journal Title

      Biochem.Biophys.Res.Commun. 335(2)

      Pages: 277-285

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Enhanced hippocampal acetylcholine release in nociceptin-receptor knockout mice.2005

    • Author(s)
      Kayoko Uezu, et al.
    • Journal Title

      Brain Res. 1050(1-2)

      Pages: 118-123

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Cloning and expression of ligand-gated ion-channel receptor L2 in central nervous system.2005

    • Author(s)
      Takeshi Houtani
    • Journal Title

      Biochem Biophys Res Commun. 335(2)

      Pages: 277-285

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Mouse homolog of KIAA0143 protein : hearing deficit induces specific changes of expression in auditory brainstem neurons.2004

    • Author(s)
      Yumi Munemoto
    • Journal Title

      Molec. Brain Res. 128(2)

      Pages: 131-140

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Lack of nociceptin receptor alters body temperature during resting period in mice.2004

    • Author(s)
      Kayoko Uezu
    • Journal Title

      NeuroReport 15(5)

      Pages: 751-755

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Mouse homolog of KIAA0143 protein : hearing deficit induces specific changes of expression in auditory brainstem neurons.2004

    • Author(s)
      Yumi Munemoto, et al.
    • Journal Title

      Molec.Brain Res. 128(2)

      Pages: 131-140

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Lack of nociceptin receptor alters body temperature during resting period in mice.2004

    • Author(s)
      Kayoko Uezu, et al.
    • Journal Title

      NeuroReport 15(5)

      Pages: 751-755

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Mouse homolog of KIAA0143 protein : hearing deficit induces specific changes of expression in auditory brainstem neurons.2004

    • Author(s)
      Yumi Munemoto
    • Journal Title

      Molec.Brain Res. 128(2)

      Pages: 131-140

    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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