| Project/Area Number |
16500251
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | National Institute for Physiological Sciences |
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Principal Investigator |
HITOSHI Seiji National Institute for Physiological Sciences, Neurobiology & Bioinformatics, Associate Professor, 分子生理研究系, 助教授 (70300895)
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| Co-Investigator(Kenkyū-buntansha) |
MARUTA Noriko Hitotsubashi University, Health care center, Associate Professor, 保健管理センター, 助教授 (50343124)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2005: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2004: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | Stress / Forced swimming / Maternal separation / Adult neurogenesis / Subventricular zone / Hippocampus / Neural stem cell / Neurosphere assay / Neuroshere assay / Neuroshpere assay |
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| Research Abstract |
Adult neurogenesis, which occurs in the olfactory bulb and dentate gyrus (DG) of the hippocampus in rodents, may play significant roles in brain function. Cell proliferation in the DG is reduced after exposure to psychosocial stress, yet the molecular and cellular mechanisms underlying this reduction remain to be elucidated. In the present study, we utilize the forced swim model of stress in the mouse to determine the effects of chronic stress on neural stem cells (NSCs) in the subventricular zone (SVZ). Chronic stress decreased the number of NSCs in the SVZ, the decrease persisted for weeks after the cessation of stress, but was reversed by treatment with antidepressant drugs. The effects of chronic stress on NSCs are blocked by bilateral adrenalectomy in vivo, and corticosterone attenuates neurosphere formation by adult NSCs in vitro. In contrast, serotonin increased survival of the NSCs when exposure was in vitro and expanded the size of NSC pool in the SVZ when serotonin was infused to the lateral ventricle in vivo. These results suggest that the number of NSCs under chronic stress conditions are regulated by the balance between the action of glucocorticoids and serotonin and provide an insight to the molecular mechanisms underlying pharmacological action of antidepressant drugs.
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