Dissection of roles of Densin-180, a cell polarity molecule, in synapses
| Project/Area Number |
16500257
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | Aichi Cancer Center |
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Principal Investigator |
IZAWA Ichiro Aichi Cancer Center, Division of Biochemistry, Senior Researcher, 発がん制御研究部, 主任研究員 (20311441)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2005: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2004: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | neuroscience / brain / development / signal transduction / dementia / Densin-180 / ERBIN / delta-catenin / 神経シナプス / PSD / LAP蛋白質 / Scribble |
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| Research Abstract |
To dissect the function of Densin-180, we overexpressed various mutants of Densin-180 in cultured cells and observed the intracellular localization of Densin-180. When a mutant lacking the leucine-rich repeats was overexpressed in Neuro2a cells, the mutant did not localize at the plasma membrane, but localized in the cytoplasm. When the C-terminal region of Densin-180, including the PDZ domain, was overexpressed in HeLa cells, the mutant localized on stress fiber. We also found that the residues cysteine 14 and cysteine 16 in the N-terminal region (1-32 amino acids) of ERBIN, a homologue of Densin-180, is important for the localization of ERBIN at the plasma membrane. We produced the anti-phospho-Densin-180 antibody which recognized the site phosphorylated by CaMKII. The antibody could detect the phosphorylation of overexpressed Densin-180 after ionomycin treatment. It was reported that in the mutant of C.elegans let-413, a homologue of Densin-180, AJM-1, a protein that showed homology to trichohyalin and plectin, was mislocalized. We identified trichoplein, a novel protein that showed homology to trichohyalin and plectin, as a keratin-binding protein.
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Report
(3 results)
Research Products
(15 results)
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[Journal Article] Identification of trichoplein, a novle keratin filament-binding protein.2005
Author(s)
Nishizawa, M., Izawa, I., Inoko, A., Hayashi, Y., Nagata, K.-i., Yokoyama, T., Usukura, J., Inagaki, M.
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Journal Title
J.Cell Sci. 118(5)
Pages: 1081-1090
Description
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[Journal Article] Aurora-B and Rho-kinase/ROCK, the two cleavage furrow kinases, independently regulate the progression of cytokinesis : possible existence of a novel cleavage furrow kinase phosphorylates ezrin/radixin/moesin (ERM).2005
Author(s)
Yokoyama, T., Goto, H., Izawa, I., Mizutani, H., Inagaki, M.
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Journal Title
Genes Cells 10(2)
Pages: 127-137
Description
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[Journal Article] PKCε-mediated phosphorylation of vimentin controls integrin recycling and motility.2005
Author(s)
Ivaska, J., Vuoriluoto, K., Huovinen, T., Izawa, I., Inagaki, M., Parker, P.J.
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Journal Title
EMBO J. 24(22)
Pages: 3834-3845
Description
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