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Urothelium releases neuro-like chemical substances for micturition reflex

Research Project

Project/Area Number 16500258
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Neurophysiology and muscle physiology
Research InstitutionAkita University

Principal Investigator

KAWATANI Masahito  Akita Univ., School of Medicine, Neurophysiology, Professor, 医学部, 教授 (00177700)

Co-Investigator(Kenkyū-buntansha) MOMOTA Y.  Akita Univ., School of Medicine, Neurophysiology, Assistant Professor, 医学部, 助手 (70295236)
YANASE Y.  Akita Univ., School of Medicine, Neurophysiology, Assistant Professor, 医学部, 助手 (10400483)
中村 靖夫  秋田大学, 医学部, 助手 (20322117)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2004: ¥2,400,000 (Direct Cost: ¥2,400,000)
KeywordsUrothelium / α_<1D> Receptor / EP_1 Receptor / Primary afferent / Unit recording / Primary afferent / Unit recording / α_<1D> Receptor / 求心性線維 / プロスタグランジン / EP1受容体 / α1受容体 / ATP / 膀胱炎
Research Abstract

Previous studies revealed that activation of alpha_1 adrenergic receptors in urothelial cells releases neurotransmitters. This study was undertaken to determine if alpha_<1D> adrenergic receptors are expressed in the urothelium of the rat bladder and if inhibition of these receptors affects reflex voiding. Female Wistar rats were used for the experiments. Receptor expression was evaluated by Western blot. The effects of receptor activation were studied using cystometrograms, measurement of ATP concentrations in the bladder lumen and afferent nerve recording. The alpha_<1D> antagonist, naftopidil (0.75-1.66 mg/kg) was administered intravenously into the external jugular vein. Expression of alpha_<1D> adrenergic receptors was detected in urothelial tissue with Westernblot and Immunohistochemistry. The alpha_<1D> receptor antagonist, naftopidil prolonged the intercontraction interval (ICI, 143 % of the control) during continuous infusion cystometrograms in conscious rats, and suppressed the excitatory effect of intravesical infusion of acetic acid (0.1 %) on the ICI (220 %). Naftopidil inhibited bladder afferent nerve activity induced by bladder distension (32.0%) or acetic acid infusion (30.4 %) and reduced the ATP levels in the bladder perfusate during bladder distension (36.6 %). Endogenous catecholamines appear to act on alpha_<1D> receptors in the urothelium to facilitate the mechano-sensitive bladder afferent nerve activity and reflex voiding.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (17 results)

All 2006 2005 2004

All Journal Article (17 results)

  • [Journal Article] Effects of loxoprofen sodiumon the micturition reflex in conscious and anesthetized mail rats.2006

    • Author(s)
      Shinozaki S, Saito M, Kawatani M
    • Journal Title

      Autonomic Neuroscience : Basic and Clinical (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Prostaglandin facilitates afferent nerve activity via EP_1 receptors during urinary bladder inflammation in rats.2006

    • Author(s)
      Ikeda M, Kawatani M, Maruyama T, Ishihama H
    • Journal Title

      Biomedical Res (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Activation of alpha1D adrenergic receptors in the rat urothelium facilitates the micturition reflex.2006

    • Author(s)
      Ishihama H, Momota Y, Yanase H, Xiaojun W, de Groat WC, Kawatani M.
    • Journal Title

      J.Urology 175

      Pages: 358-364

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Activation of α1D adrenergic receptors in the rat urothelium facilitates the micturition reflex.2006

    • Author(s)
      Ishihama H, Momota Y, Yanase H, Xiaojun W, de Groat WC, Kawatani M.
    • Journal Title

      J.Urology 175

      Pages: 358-364

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Effects of loxoprofen sodiumon the micturition reflex in conscious and anesthetized mail rats.2006

    • Author(s)
      Shinozaki S, Saito M, Kawatani M.
    • Journal Title

      Autonomic Neuroscience : Basic and Clinical (in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Prostaglandin facilitates afferent nerve activity via EP_1 receptors during urinary bladder inflammation in rats2006

    • Author(s)
      Ikeda M, Kawatani M, Muruyama T, Ishihama H
    • Journal Title

      Biomedical Res (in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Loxoprofen inhibits facilitated micturition reflex induced by acetic acid urinary bladder infusion of the rat.2005

    • Author(s)
      Shinozaki S, Saito M, Kawatani M
    • Journal Title

      Biomedical Res 26

      Pages: 29-33

    • NAID

      130004903691

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Effectiveness of an anti-inflammatory drug,loxoprofen,for patients with nocturia.2005

    • Author(s)
      Saito M, Kawatani M, Kinoshita Y, Satoh K, Miyagawa I
    • Journal Title

      Int.J Urology 12

      Pages: 779-782

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Activation of α 1D adrenergic receptors in the rat urothelium facilitates the micturition reflex.2005

    • Author(s)
      Ishihama H, Momota Y, Yanase H, Xiaojun W, de Groat WC, Kawatani M.
    • Journal Title

      J.Urology 175

      Pages: 358-364

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Losoprofen inhibits facilitated micturition reflex induced by acetic acid urinary bladder infusion of the rat.2005

    • Author(s)
      Shinozaki S, Saito M, Kawatani M.
    • Journal Title

      Biomedical Res 26

      Pages: 29-33

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Effectiveness of an anti-inflammatory drug, loxoprofen, for patients with nocturia.2005

    • Author(s)
      Saito M, Kawatani M, Kinoshita Y, Satoh K., Miyagawa I.
    • Journal Title

      Int.J Urology 12

      Pages: 779-782

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Effectiveness of an anti-inflammatory drug, loxoprofen, for patients with nocturia.2005

    • Author(s)
      Saito M, Kawatani M, Kinoshita Y, Satoh K, Miyagawa I
    • Journal Title

      Int.J Urology 12

      Pages: 779-782

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 膀胱求心性神経活動からみた過活動膀胱2004

    • Author(s)
      河谷正仁, 池田 真, 石濱寛子
    • Journal Title

      自律神経 41巻3号

      Pages: 316-319

    • NAID

      10013597206

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Urothelium could be important for vesical pain2004

    • Author(s)
      Masahito Kawatani
    • Journal Title

      Pain Research 19巻5号

      Pages: 185-190

    • NAID

      10014246010

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] 排尿メカニズムにおける膀胱知覚神経系の役割2004

    • Author(s)
      河谷正仁
    • Journal Title

      排尿障害プラクティス 12巻4号

      Pages: 265-269

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Urothelium could be important for vesical pain.2004

    • Author(s)
      Kawatani M.
    • Journal Title

      Pain Res 19

      Pages: 185-190

    • NAID

      10014246010

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Characterization of prostaglandin E receptor subtypes involved in the relaxation of rabbit penile corpus cavernosum smooth muscle.2004

    • Author(s)
      Sato M, Kawatani M.
    • Journal Title

      Biomedical Res 25

      Pages: 237-244

    • NAID

      130004470629

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary

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Published: 2004-04-01   Modified: 2016-04-21  

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