Functional analysis of purinergic receptors using IMCT technology in transgenic mouse retina

• Project/Area Number: 16500268
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurophysiology and muscle physiology
• Research Institution: Keio University
• Principal Investigator: KANEDA Makoto Keio University, Assistant Professor, 医学部, 助教授 (30214480)
• Co-Investigator(Kenkyū-buntansha): KANEKO Akimichi Seijo University, Professor, リハビリテーション学部, 教授 (00051491)
• Project Period (FY): 2004 – 2005
• Project Status: Completed (Fiscal Year 2005)
• Budget Amount: ¥3,200,000 (Direct Cost: ¥3,200,000); Fiscal Year 2005: ¥800,000 (Direct Cost: ¥800,000); Fiscal Year 2004: ¥2,400,000 (Direct Cost: ¥2,400,000)
• Keywords: retina / ATP / purinergic receptors / マウス

Research Abstract

We investigated the distribution of P2X-purinoceptor families in the cholinergic amacrine cells of the mouse retina by the immunohistochemical technique and the patch clamp technique. The immunoreactivity for P2X2-purinoceptors selectively distributed in the OFF-type cholinergic amacrine cells but not in the ON-type cholinergic amacrine cells. The immunoreactivity for P2X2-purinoceptors in the OFF-type cholinergic amacrine cells disappeared when IMCT technology was introduced to ablate the cholinergic amacrine cells selectively from the retinal circuit. These immunohistochemical studies indicate that P2X2-purinoceptors are selectively expressed on the OFF-type cholinergic amacrine cells. In the slice preparation of the transgenic mouse retina, the cholinergic amacrine cells were identified by the signals of green fluorescent protein. Under the patch clamp recordings, responses to ATP in the OFF-type cholinergic amacrine cells were greater than those in the ON-type cholinergic amacrine cells. No responses to ATP were observed in the non-cholinergic amacrine cells. The responses of ATP in the OFF-type cholinergic amacrine cells were mediated by the P2X2-purinoceptors. The electrophysiological data also support that P2X2-purinoceptors are selectively expressed in the OFF-type cholinergic amacrine cells. ATP also increased the GABAergic inputs to the dendrites of both the ON-type and the OFF-type cholinergic amacrine cells. The increase in the GABAergic inputs were mediated by the different types of P2X-purinoceptors.

Research Products

• [Journal Article] Shinkeisaibou no Denkiseiri (2006)
  • Author(s): Kaneda, M.
  • Journal Title: Nousinnkei Gekagaku Taikei 1 Pages: 112-120
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Endogenous zinc can be a modulator of glycinergic signaling pathway in the rat retina. (2005)
  • Author(s): Kaneda, M., Ishii, K., Akagi, T., Tastukawa, T., Hashikawa, T.
  • Journal Title: J. Mol.Histol. 36 Pages: 179-185
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] GABA-mediated component in the feedback response of turtle retinal cones. (2005)
  • Author(s): Tatsukawa, T., Hirasawa, H., Kaneko, A., Kaneda, M.
  • Journal Title: Vis. Neurosci. 22 Pages: 317-324
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Electrophysiological analysis of the cell : patch-clamp recording (2005)
  • Author(s): Yamaguchi, K., Kaneda, M.
  • Journal Title: Tanpakusitsu Kakusan Koso 49 Pages: 1628-1633
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Endogenous zinc can be a modulator of glycinergic signaling pathway in the rat retina. (2005)
  • Author(s): Kaneda, M., Ishii, K., Akagi, T., Tastukawa, T., Hashikawa, T.
  • Journal Title: J.Mol.Histol. 36 Pages: 179-185
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] GABA-mediated component in the feedback response of turtle retinal cones. (2005)
  • Author(s): Tatsukawa, T., Hirasawa, H., Kaneko, A., Kaneda, M.
  • Journal Title: Vis.Neurosci. 22 Pages: 317-324
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Endogenous zinc can be a modulator of glycinergic signaling pathway in the rat retina. (2005)
  • Author(s): Kaneda, M., Ishii, K., Akagi, T., Tatsukawa, T., Hashikawa, T.
  • Journal Title: J.Mol.Histol. 36 Pages: 179-185
• [Journal Article] OFF-cholinergic-pathway-selective localization of P2X2-purinoceptors in the mouse retina. (2004)
  • Author(s): Kaneda, M., Ishii, K., Morishima, Y., Akagi, T., Yamazaki, Y., Nakanishi, S., Hashikawa, T.
  • Journal Title: J. Comp. Neurol. 476 Pages: 103-111
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] A nonsense mutation of the sodium channel gene SCN2A in apatient with intractable epilepsy and mental decline. (2004)
  • Author(s): Kamiya, K., Kaneda, M., Sugawara, T., Mazaki, E., Okamura, N., Montal, M., Makita, N, Tanaka, M., Fukushima, K., Fujiwara, T., Inoue, Y., Yamakawa, K.
  • Journal Title: J. Neurosci. 24 Pages: 2690-2698
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] 細胞の電気生理学的解析 : パッチクランプ法 (2004)
  • Author(s): 山口和彦, 金田 誠
  • Journal Title: 蛋白質・核酸・酵素 49 Pages: 1628-1633
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] OFF-cholinergic-pathway-selective localization of P2X2-purinoceptors in the mouse retina. (2004)
  • Author(s): Kaneda, M., Ishii, K., Morishima, Y.Akagi, T, Yamazaki, Y., Nakanishi, S., Hashikawa, T.
  • Journal Title: J.Comp.Neurol. 476 Pages: 103-111
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A nonsense mutation of the sodium channel gene SCN2A in a patient with intractable epilepsy and mental decline. (2004)
  • Author(s): Kamiya, K., Kaneda, M., Sugawara, T., Mazaki, E., Okamura, N., Montal, M., Makita, N, Tanaka, M., Fukushima, K., Fujiwara, T., Inoue, Y., Yamakawa, K.
  • Journal Title: J.Neurosci. 24 Pages: 2690-2698
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] OFF-cholinergic-pathway-selective localization of P2X2-purinoceptors in the mouse retina. (2004)
  • Author(s): Kaneda, M., Ishii, K., Morishima, Y., Akagi, T., Yamazaki, Y., Nakanishi, S., Hashikawa T.
  • Journal Title: J.Comp.Neurol. 476 Pages: 103-111
• [Journal Article] 細胞の電気生理学的解析:パッチクランプ法 (2004)
  • Author(s): 山口和彦, 金田 誠
  • Journal Title: 蛋白質・核酸・酵素 49 Pages: 1628-1633
• [Journal Article] Endogenous zinc can be a modulator of glycinergic signaling pathway in the rat retina
  • Author(s): Kaneda, M., Ishii, K., Akagi, T., Tastukawa, T., Hashikawa, T.
  • Journal Title: J.Mol.Histol. (In press)
• [Journal Article] 神経細胞の電気生理
  • Author(s): 金田 誠
  • Journal Title: 脳神経外科学大系(神経科学) 1(印刷中)
• [Book] 神経細胞の電気生理(脳神経外科学体系1巻 神経科学) (2006)
  • Author(s): 金田 誠
  • Total Pages: 386
  • Publisher: 中山書店
  • Description: 「研究成果報告書概要(和文)」より