| Project/Area Number |
16500274
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
MIURA Ryuichi The University of Tokyo, Institute of Medical Science, Research Associate, 医科学研究所, 助手 (00322074)
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| Co-Investigator(Kenkyū-buntansha) |
KAI Chieko The University of Tokyo, Institute of Medical Science, Professor, 医科学研究所, 教授 (10167330)
KOHARA Kyoko The University of Tokyo, Institute of Medical Science, Lecturer, 医科学研究所, 講師 (20225478)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2005: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2004: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | measles virus / transgenic mice / receptor / heparin sulfate / トランスジェニックマウス |
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| Research Abstract |
Since only primate model is available for research of measles virus (MV) infection, transgenic mice expressing human SLAM (SLAM-Tg mice), one of MV receptors, is expected to be a useful tool for analysis of its pathogenicity. It is also important to search other receptors mediating MV infection because SLAM expression is restricted to lymphocytes. In this project, we prepared human SLAM cDNA fragments for generation of SLAM Tg mice. The SLAM expression was controlled by lck gene promoter or IgH gene promoter that is specifically activated in lymphocytes and finally, 3 (in 48) or 14 (in 137) founder mice were obtained respectively. Although 3 lines of Tg mice whose gene was driven by IgH promoter produced next generation, no expression of human SLAM mRNA was detected in splenocyte which contained abundant lymphocytes and no remarkable pathogenicity was observed in the MV-infected Tg mice as well as in their control mice. In analyses of SLAM-negative cell lines, MV infectivity was decreased by pre-treatment of soluble heparin or heparitinase. Moreover, the infectivity of heparin sulfate-defective cell line was also lower than that of control cell line and MV particle was absorbed on surfaces of heparin binding beads. These results indicate that heparin sulfate strongly mediated MV infection of SLAM-negative cells.
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