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Establishment and analysis of measles virus-susceptible transgenic mice.

Research Project

Project/Area Number 16500274
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory animal science
Research InstitutionThe University of Tokyo

Principal Investigator

MIURA Ryuichi  The University of Tokyo, Institute of Medical Science, Research Associate, 医科学研究所, 助手 (00322074)

Co-Investigator(Kenkyū-buntansha) KAI Chieko  The University of Tokyo, Institute of Medical Science, Professor, 医科学研究所, 教授 (10167330)
KOHARA Kyoko  The University of Tokyo, Institute of Medical Science, Lecturer, 医科学研究所, 講師 (20225478)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2005: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2004: ¥1,900,000 (Direct Cost: ¥1,900,000)
Keywordsmeasles virus / transgenic mice / receptor / heparin sulfate / トランスジェニックマウス
Research Abstract

Since only primate model is available for research of measles virus (MV) infection, transgenic mice expressing human SLAM (SLAM-Tg mice), one of MV receptors, is expected to be a useful tool for analysis of its pathogenicity. It is also important to search other receptors mediating MV infection because SLAM expression is restricted to lymphocytes. In this project, we prepared human SLAM cDNA fragments for generation of SLAM Tg mice. The SLAM expression was controlled by lck gene promoter or IgH gene promoter that is specifically activated in lymphocytes and finally, 3 (in 48) or 14 (in 137) founder mice were obtained respectively. Although 3 lines of Tg mice whose gene was driven by IgH promoter produced next generation, no expression of human SLAM mRNA was detected in splenocyte which contained abundant lymphocytes and no remarkable pathogenicity was observed in the MV-infected Tg mice as well as in their control mice. In analyses of SLAM-negative cell lines, MV infectivity was decreased by pre-treatment of soluble heparin or heparitinase. Moreover, the infectivity of heparin sulfate-defective cell line was also lower than that of control cell line and MV particle was absorbed on surfaces of heparin binding beads. These results indicate that heparin sulfate strongly mediated MV infection of SLAM-negative cells.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (16 results)

All 2006 2005 2004

All Journal Article (16 results)

  • [Journal Article] Characterization of monoclonal antibodies directed against the canine distemper virus nucleocapsid protein.2006

    • Author(s)
      Masuda M et al.
    • Journal Title

      Comp Immunol Microbiol Infect Dis 29(2-3)

      Pages: 157-165

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Characterization of monoclonal antibodies directed against the canine distemper virus nucleocapsid protein.2006

    • Author(s)
      Masuda M et al.
    • Journal Title

      Comp.Immunol.Microbiol.Infect.Dis. 30(in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Measles virus infection induces release of interleukin-8 in human pulmonary epithelial cells.2005

    • Author(s)
      Sato H et al.
    • Journal Title

      Comp Immunol Microbiol Infect Dis 28(4)

      Pages: 311-320

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Expression and purification of recombinant swine interleukin-4.2005

    • Author(s)
      Nuntaprasert A et al.
    • Journal Title

      Comp Immunol Microbiol Infect Dis 28(1)

      Pages: 17-35

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Expression and characterization of the recombinant swine interleukin-6.2005

    • Author(s)
      Nuntaprasert A et al.
    • Journal Title

      Comp Immunol Microbiol Infect Dis 28(2)

      Pages: 103-120

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Measles virus infection induces release of interleukin-8 in human pulmonary epithelial cells.2005

    • Author(s)
      Sato H et al.
    • Journal Title

      Comp.Immunol.Microbiol.Infect.Dis. 29

      Pages: 311-320

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Expression and characterization of the recombinant swine interleukin-6.2005

    • Author(s)
      Nuntaprasert A et al.
    • Journal Title

      Comp Immunol Miscobiol Infect Dis 28(2)

      Pages: 103-120

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Expression and purification of recombinant swine interleukin-4.2005

    • Author(s)
      Nuntaprasert A et al.
    • Journal Title

      Comp Immunol Microbiol Infact Dis 28(1)

      Pages: 17-35

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Phylogenetic analysis of the hemagglutinin (H) gene of canine distemper viruses isolated from wild masked palm civets (Paguma larvata).2004

    • Author(s)
      Hirama K et al.
    • Journal Title

      J Vet Med Sci 66(12)

      Pages: 1575-1578

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Prevalence of canine distemper virus, feline immunodeficiency virus and feline leukemia virus in captive African lions (Panthera leo) in Japan.2004

    • Author(s)
      Endo Y et al.
    • Journal Title

      J Vet Med Sci 66(12)

      Pages: 1587-1589

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Rinderpest virus phosphoprotein gene is a major determinant of species-specific pathogenicity.2004

    • Author(s)
      Yoneda M et al.
    • Journal Title

      J Virol 78(12)

      Pages: 6676-6681

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Characterization of specific antibodies and the establishment of sandwich ELISA and ELISPOT systems for swine IL-4.2004

    • Author(s)
      Nuntaprasert A et al.
    • Journal Title

      Comp Immunol Microbiol Infect Dis 27(6)

      Pages: 457-470

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Rinderpest virus phosphoprotein gene is a major determinant of species-specific pathogenicity.2004

    • Author(s)
      Yoneda M et al.
    • Journal Title

      J Viol 78(12)

      Pages: 6676-6681

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Characterization of specific antibodies and the establishment of sandwich ELISA and ELISPOT systems for swine IL-4.2004

    • Author(s)
      Nuntaprasert A et al.
    • Journal Title

      Comp Immmunol Microbiol Infact Dis 27(6)

      Pages: 457-470

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Phylogenetic analysis of the hemagglutinin (H) gene of canine distemper viruses isolated from wild masked palm civets(Paguma larvata).2004

    • Author(s)
      Hirama K et al.
    • Journal Title

      J Vet Med Sci 66(12)

      Pages: 1575-1578

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Prevalence of canine distemper virus, feline immunodeficiency virus and feline leukemia virus in captive African lions (Panthera leo)in Japan.2004

    • Author(s)
      Endo Y et al.
    • Journal Title

      J Vet Med Sci 66(12)

      Pages: 1587-1589

    • Related Report
      2004 Annual Research Report

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Published: 2004-04-01   Modified: 2016-04-21  

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