| Project/Area Number |
16500275
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Nagoya University |
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Principal Investigator |
YAMAGATA Takahiro Nagoya University, Graduate School of Bioagricultural Sciences, Assistant Professor, 大学院・生命農学研究科, 助手 (50242847)
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| Co-Investigator(Kenkyū-buntansha) |
ISHIKAWA Akira Nagoya University, Graduate School of Bioagricultural Sciences, Associate Professor, 大学院・生命農学研究科, 助教授 (20211724)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2005: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2004: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | wild mice / genetic resources / mutant / spontaneous disease model / congenic strain / recombinant inbred strains |
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| Research Abstract |
A purpose of this research is to discover useful mutant genes from genetic resources of wild mice, development of new spontaneous disease models from them and analyze their characteristic and heredity. We have established a new inbred line (CASP) from Philippine wild mice. And we discovered some mutants from the mating of the wild mice with existing inbred strains and bred them as lines. Research results provided during a research period are as follows. About a search of genetic characteristics of the new inbred strain, we performed polymorphism analysis using by microsatellite markers. As a result, it was different from that of existing inbred strains greatly and we confirmed it is useful for genetic analysis of the mutants discovered. About a breeding of mutant lines, an inbred line expressed stable hyperglycemia was established as an non-insulin dependent diabetes mellitus model by a selective inbreeding. We make a mating panel with this line and an existing inbred strains, and then
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we will perform genetic analysis about this hyperglycemia factor. Morphosis abnormality mice have been bred as a congenic line and we can go ahead with a goal of inbreeding. We investigated characteristics of these lines and it was predicted that they were new mutants which were not reported. About body size variant lines, we performed a functional analysis and refinement of candidate genes about mapped quantitative trait loci (QTLs) concerning body weight and growth of mice by making congenic strains. We reported the characteristic and expression patterns of these QTLs as novel genes influenced weight difference. About development and breeding of new recombinant inbred (RI) strains by mating between subspecies, we started the mating between CASP and BALB/cA strains and have been bred each of more than 15 lines from two F1 pairs with reciprocal mating. However, as reproductive performance fell off in many lines between F several and F ten and several generations, it is difficult to establish more than 20 lines in total as inbred strains finally. This cause investigation and measures are future examination problems. Less
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