| Project/Area Number |
16500324
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Medical systems
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| Research Institution | Meiji University, School of Science and Technology |
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Principal Investigator |
HIRAOKA Wakako Meiji University, School of Science and Technology, Associate Professor, 理工学部, 助教授 (00212168)
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| Co-Investigator(Kenkyū-buntansha) |
CHOI Pak-Kon Meiji University, School of Science and Technology, Professor, 理工学部, 教授 (30143530)
KONDO Takashi University of Toyama, Faculty of Medicine, Professor, 医学部, 教授 (40143937)
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| Project Period (FY) |
2004 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2005: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2004: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | sonotherapy / skin physiology / homeostasis / melanoma / cell culture / tyrosine / melanin / radical scavenger / ラジカルスカベンジャー / 皮膚生理 / 紫外線 / UV-A |
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| Research Abstract |
Homeostasis of skin physiology is maintained by the subcutaneous tissue structure and the normal turnover of hypodermically division cell. Change of skin environment disturb these skin physiology, and give a damage to subcutaneous tissue. This research aimed to develop sonotherapy system for maintenance of skin homeostasis.
1.Ultrasonic Generator : PZT element attached to the bottom of irradiation container was connected by an amplifier and generator (IWATSU, SG-4105 and NF HSA4101). This system produced 1-7 MHz ultrasound.
2.Influence to Cell growth : In order to estimate the safety of the created equipment, the influence of ultrasound to cell growth was investigated. U937 cell, human lymphoma cell, was irradiated with 1-7 MHz ultrasound. Dependencies of sound power and irradiation time were analyzed.
3.Inactivation of mushroom tyrosinase. : When the enzyme solution was irradiated with 1 MHz ultrasound at intensity 0.47 W/cm^2 or less, tyrosinase activity was reduced during irradiation and then recovered immediately after irradiation. On the other hand, the enzyme was irradiated at intensity greater than 0.47 W/cm^2, the reduction of tyrosinase activity was continued after irradiation. Experiment of ESR-spin trapping with dimethyl-1-pyrroline N-oxide showed the production of hydroxyl radical in US-irradiated buffer solution at intensity rather than 0.47 W/cm^2. Although 0.25 M mannitol scavenged hydroxyl radical in US-irradiated buffer solution, the effect of mannitol on the US-induced inactivation of tyrosinase was not observed. While neither catalase nor SOD showed the effect on enzyme activity after US-irradiation, in the presence of Cu^<2+> (10^<-6>M〜10^<-4>M), ultrasound did not induce the inactivation of tyrosinase. These results suggest that the mechanism of US-induced inactivation of tyrosinase is based on Cu^<2+> release from reactive center of the enzyme rather than US-induced ROIs.
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