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Molecular mechanisms underlying exercise training-induced reduction of aortic stiffness

Research Project

Project/Area Number 16500391
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Sports science
Research InstitutionUniversity of Tsukuba

Principal Investigator

MAEDA Seiji  University of Tsukuba, Graduate School of Comprehensive Human Sciences, Assistant Professor, 大学院・人間総合科学研究科, 講師 (30282346)

Co-Investigator(Kenkyū-buntansha) IEMITSU Motoyuki  University of Tsukuba, Graduate School of Comprehensive Human Sciences, Research Associate, 大学院・人間総合科学研究科, 助手 (90375460)
MIYAUCHI Takashi  University of Tsukuba, Graduate School of Comprehensive Human Sciences, Professor, 大学院・人間総合科学研究科, 教授 (60222329)
Project Period (FY) 2004 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2005: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2004: ¥2,300,000 (Direct Cost: ¥2,300,000)
Keywordsaorta / exercise training / mRNA / vasoactive factor / arterial stiffness / heart / プロスタグランディン / CNP / eNOS / マイクロアレイ / 遺伝子
Research Abstract

Regular aerobic exercise reduces aortic stiffness. However, the mechanisms by which chronic exercise lowers arterial stiffness are not known. To determine the molecular mechanisms of these changes, the alteration of gene expression in the aorta by aerobic exercise training was measured with the microarray technique. The differences in expression levels of 3,800 genes in the abdominal aorta of sedentary control (8 weeks old) and exercise-trained rats (8 weeks old, treadmill running for 4 weeks) were compared by the microarray analysis. Aortic pulse wave velocity (PWV) was lower and systemic arterial compliance was higher (both P<0.05) in the exercise-trained group than in the control group. Of the 323 genes that displayed differential expression (upregulation of 206 genes and downregulation of 117 genes), a total of 29 genes (24 upregulated and 5 downregulated genes) were identified as potential candidate genes that may be involved in vasodilation and arterial destiffening. Using real-time quantitative PCR, we confirmed the results of microarray analysis that prostaglandin EP2 receptor (PGE-EP2R), prostaglandin EP4 receptor (PGE-EP4R), C-type natriuretic peptide (CNP), and endothelial nitric oxide synthase (eNOS) genes were differentially expressed. Furthermore, there were modest correlations between arterial stiffness and levels of these factors. Differential expression of eNOS gene was further verified at protein level by using Western blot analysis. These results suggest that exercise training induces the altered expression in several genes including prostaglandin, CNP, and nitric oxide in the aorta and that these molecular changes (particularly eNOS as its protein expression was altered) may contribute, at least in part, to the beneficial effect of exercise training on aortic stiffness.

Report

(3 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • Research Products

    (20 results)

All 2005 2004 Other

All Journal Article (20 results)

  • [Journal Article] Aortic stiffness and aerobic exercise : mechanistic insight from microarray analyses2005

    • Author(s)
      Maeda S, et al.
    • Journal Title

      Medicine & Science in Sports & Exercise 37

      Pages: 1710-1716

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Gene expression profiling of exercise-induced cardiac hypertrophy in rats2005

    • Author(s)
      Iemitsu M, et al.
    • Journal Title

      Acta Physiologica Scandinavica 185

      Pages: 259-270

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] トレーニングに対する血管応答2005

    • Author(s)
      前田清司
    • Journal Title

      体育の科学 55

      Pages: 907-912

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Aortic stiffness and aerobic exercise : mechanistic insight from microarray analyses.2005

    • Author(s)
      Seiji Maeda, et al.
    • Journal Title

      Medicine & Science in Sports & Exercise 37

      Pages: 1710-1716

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Gene expression profiling of exercise-induced cardiac hypertrophy in rats.2005

    • Author(s)
      Motoyuki Iemitsu, et al.
    • Journal Title

      Acta Physiologica Scandinavica 185

      Pages: 259-270

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Exercise training and vascular response.2005

    • Author(s)
      Seiji Maeda
    • Journal Title

      Journal of Health, Physical Education and Recreation 55

      Pages: 907-912

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Aortic stiffiness and aerobic exercise : mechanistic insight from microarray analyses2005

    • Author(s)
      Maeda S, et al.
    • Journal Title

      Medicine & Science in Sports & Exercise 37

      Pages: 1710-1716

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Acute exercise causes an enhancement of tissue renin-angiotensin system in the kidney in rats2005

    • Author(s)
      Maeda S, et al.
    • Journal Title

      Acta Physiologica Scandinavica 185

      Pages: 79-86

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Moderate regular exercise increases basal production of nitric oxide in elderly women.2004

    • Author(s)
      Maeda S, et al.
    • Journal Title

      Hypertension Research 27

      Pages: 947-953

    • NAID

      10014466824

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Endothelin receptor antagonist reverses decreased NO system in the kidney in vivo during exercise.2004

    • Author(s)
      Maeda S, et al.
    • Journal Title

      American Journal of Physiology : Endocrinology and Metabolism 286

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Exercise-induced tissue-specific change in gene expression of endothelin-1.2004

    • Author(s)
      Maeda S, et al.
    • Journal Title

      Journal of Cardiovascular Pharmacology 44

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Exercise training improves cardiac function-related gene levels through thyroid hormone receptor signaling in aged rats.2004

    • Author(s)
      Iemitsu M, Maeda S, et al.
    • Journal Title

      American Journal of Physiology : Heart and Circulatory Physiology 286

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Effects of nitric oxide synthase inhibitor on decrease in peripheral arterial stiffness with acute low intensity aerobic exercise.2004

    • Author(s)
      Sugawara J, Maeda S, et al.
    • Journal Title

      American Journal of Physiology : Heart and Circulatory Physiology 287

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Time course alteration of endothelin-1 gene expression in the heart during exercise and recovery from post-exercise periods in rats.2004

    • Author(s)
      Iemitsu M, Maeda S, et al.
    • Journal Title

      Journal of Cardiovascular Pharmacology 44

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Individual variations of exercise training-induced physiological effects and genetic factor

    • Author(s)
      Maeda S, et al.
    • Journal Title

      International Journal of Sport and Health Sciences (in press)

    • NAID

      130000069793

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Gene expression profiling of exercise training-induced cardiovascular adaptations : molecular insight from microarray analyses

    • Author(s)
      Iemitsu M, et al.
    • Journal Title

      International Journal of Sport and Health Sciences (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Time course alterations of myocardial endothelin-1 production during the formation of exercise training-induced cardiac hypertrophy

    • Author(s)
      Iemitsu M, et al.
    • Journal Title

      Experimental Biology and Medicine (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Individual variations of exercise training-induced physiological effects and genetic factor.

    • Author(s)
      Seiji Maeda, et al.
    • Journal Title

      International Journal of Sport and Health Science (in press)

    • NAID

      130000069793

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Gene expression profiling of exercise training-induced cardiovascular adaptations : molecular insight from microarray analyses.

    • Author(s)
      Motoyuki Iemitsu, et al.
    • Journal Title

      International Journal of Sport and Health Science (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Time course alterations of myocardial endothelin-1 production during the formation of exercise training-induced cardiac hypertrophy.

    • Author(s)
      Motoyuki Iemitsu, et al.
    • Journal Title

      Experimental Biology and Medicine (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary

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Published: 2004-04-01   Modified: 2016-04-21  

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