| Project/Area Number |
16510027
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental impact assessment/Environmental policy
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| Research Institution | KURUME UNIVERSITY (2005-2006)
Tokyo Women's Medical University (2004) |
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Principal Investigator |
ISHIHARA Yoko KURUME UNIVERSITY, SCHOOL OF MEDICINE, PROFESSOR, 医学部, 教授 (50203021)
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| Co-Investigator(Kenkyū-buntansha) |
HAGIWARA Hiromi YOKOHAMATOIN UNIVERSITY, PROFESSOR, 工学部, 教授 (90189465)
TOMITA Sachiko TOKYO WOMEN'S MEDICAL UNIVERSITY, SCHOOL OF MEDICINE, ASSISTANT, 医学部, 助手 (40231451)
KOKUBO Hiroki NATIONAL GENETIC INSTITUTION, ASSISTANT, 助手 (10270480)
坂田 律 久留米大学, 医学部, 助手 (60258423)
藤田 裕規 久留米大学, 医学部, 助手 (10330797)
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| Project Period (FY) |
2004 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2005: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2004: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | Nanoparticles / Risk assessment / health effects / Molecular biology / Ultrafine particles / particle dosimetry / Diesel particulate matters / CAPs / 粒子毒性 / DNAプロファイル / サイトカイン / ナノテクノロジー / 微小粒子 / DNAファイル / DNA分析 / マクロファージ / 健康影響評価 |
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| Research Abstract |
We evaluated the usefulness of molecular biological methods in the health risk assessment of environmental nanoparticles. (1) Lung and heart tissues were collected from aged rats (aged 22-24 months) that had been exposed to diesel particles for 7 months or concentrated air particles (CAPs) for 3 days. The results of DNA profile analysis showed increases in DNA gene expression in complements, macrophage activated cytokine, chemokine, CINC-2, hemoxidase, and angiotensin II receptor in the lung exposed to diesel emission, in complements, inflammation-related components and blood coagulation-related items in the lung exposed to CAPs, and in complements in the heart exposed to CAPs. The increases in DNA expression level did not necessarily correspond with mRNA expression or protein concentration. Physiological and pathological findings and changes in DNA and mRNA expression showed only slight correlations in aged rats exposed to diesel particles or CAPs. On the other hand, the expression of inflammation-related cytokine mRNA was higher in the lungs of rats treated with diesel particles washed with organic solvent (E-DEP) than in those of rats treated with unwashed particles (DEP). E-DEP had a larger particle area per weight than DEP. These findings support the hypothesis that nanoparticles have greater toxicity in the lung than fine particles. The results of our study suggested that DNA profile analysis are useful for the selection of disease-related biomarkers in the first step of the health risk assessment of nanoparticles. Following this, the levels of mRNA expression and protein concentration are confirmed for the selected biomarkers. The final health risk assessment of nanoparticle toxicity should be based on an overall evaluation of molecular biological, biochemical, hematological, pathological and physiological data.
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